User:Z3460352: Difference between revisions

Revision as of 15:52, 13 August 2015

Lab Attendance

--Z3460352 (talk) 13:45, 7 August 2015 (AEST)

Week 1 Lab Assessment

Chromosomal Development in relation to Blastocyst Morphology

Figueira, Setti, Braga, Laconelli and Borges aimed to decipher the link between embryo morphology and chromosomal development in the early days of fertilisation. Specifically, the study focused on the structure of chromosomes within the embryo at day three of development.

For the purpose of the experiment, Intra-Cytoplasmic Sperm Injection (ICSI) cycles were run 106 times, before a genetic screening test was completed (PGS, Pre-Implantation Genetic aneuploidy Screening). This presented 596 embryos for use.

Embryonic growth was monitored closely and the genetic composition of each cell was analysed. Fluorescent In Situ Hybridisation (FISH) was used to analyse the complementary sequences of DNA within the embryos; 200 of the 564 tagged had developed into blastocysts.

Approximately 59% of the blastocysts were euploid (having an even set of chromosomes), whereas a lesser proportion of embryos that had not become blastocysts were euploid (41.2%). Furthermore, abnormalities in blastocyst development were observed, finding that if an embryo was an euploid, it would most likely have a normal inner cell mass (ICM). In contrast, aneuploidy embryos were found to be those with abnormal ICMs. A similar conclusion was found in the observation of trophectoderm morphology in that euploid embryos had a ‘normal’ cell distribution, whereas aneuploids did not.

Based on these findings, the study concluded that embryo development is not hindered by genetic abnormalities in the early stages of development. ICM morphology presents a stronger link to chromosomal abnormalities as the majority of aneuploidy embryos had abnormal ICMs.

Although this study presented clear links between embryonic development and genetic abnormalities, further studies could be conducted to strengthen these connections.

PMID 26246880

In Vitro Fertilisation in women with, and without Polycystic Ovarian Syndrome

PMID 26241855

Look at this ^[1]

↑ <pubmed>26246880</pubmed>

Please do not use your real name on this website, use only your student number.

Glossary Links

Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link

[1] <pubmed>26246880</pubmed>

[1]

@@ Line 8: / Line 8: @@
 '''''Chromosomal Development in relation to Blastocyst Morphology'''''
+Figueira, Setti, Braga, Laconelli and Borges aimed to decipher the link between embryo morphology and chromosomal development in the early days of fertilisation. Specifically, the study focused on the structure of chromosomes within the embryo at day three of development.
+For the purpose of the experiment, Intra-Cytoplasmic Sperm Injection (ICSI) cycles were run 106 times, before a genetic screening test was completed (PGS, Pre-Implantation Genetic aneuploidy Screening). This presented 596 embryos for use.
+Embryonic growth was monitored closely and the genetic composition of each cell was analysed. Fluorescent In Situ Hybridisation (FISH) was used to analyse the complementary sequences of DNA within the embryos; 200 of the 564 tagged had developed into blastocysts.
+Approximately 59% of the blastocysts were euploid (having an even set of chromosomes), whereas a lesser proportion of embryos that had not become blastocysts were euploid (41.2%).
+Furthermore, abnormalities in blastocyst development were observed, finding that if an embryo was an euploid, it would most likely have a normal inner cell mass (ICM). In contrast, aneuploidy embryos were found to be those with abnormal ICMs. A similar conclusion was found in the observation of trophectoderm morphology in that euploid embryos had a ‘normal’ cell distribution, whereas aneuploids did not.
+Based on these findings, the study concluded that embryo development is not hindered by genetic abnormalities in the early stages of development. ICM morphology presents a stronger link to chromosomal abnormalities as the majority of aneuploidy embryos had abnormal ICMs.
+Although this study presented clear links between embryonic development and genetic abnormalities, further studies could be conducted to strengthen these connections.
 PMID 26246880