From Embryology

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Human Embryo Carnegie stage 3

Human Blastocyst "hatching" from zona pellucida, in early Embryonic Development designated as Carnegie stage 3.

The zona pellucida is shown to the left of the image and the blastocyst is to the right of image.


  • the small opening in the zona pellucida through which the blastocyst is hatching
  • the flattened trophoblast cells forming the outer cell layer of the blastocyst
  • the inner cell mass shown in the centre of the image and on the left-hand wall of the blastocyst
  • the blastocoel forming a large fluid-filled space within the blastocyst

Links: unlabeled image | labeled image | Carnegie stage 3 | Blastocyst Development | Week 1


<pubmed>16929302</pubmed>| Nature


Adapted by permission from Macmillan Publishers Ltd (copyright 2006)

Original image name: Figure 1 part b, Development of a blastomere-biopsied embryo into a hatching blastocyst. (image extracted from full figure and adjusted in size and contrast)

File history

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current13:07, 9 May 2011Thumbnail for version as of 13:07, 9 May 2011500 × 377 (20 KB)S8600021 (talk | contribs)
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12:32, 2 August 2009Thumbnail for version as of 12:32, 2 August 2009285 × 219 (11 KB)S8600021 (talk | contribs)Image source: Klimanskaya I, Chung Y, Becker S, Lu SJ, Lanza R. Human embryonic stem cell lines derived from single blastomeres. Nature. 2006 Aug 23 [

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