Difference between revisions of "Ectoderm"

From Embryology
(Internet Links)
Line 202: Line 202:
 
(Stage text modified from: Neurulation in the normal human embryo. O'Rahilly R, Muller F Ciba Found Symp 1994;181:70-82)
 
(Stage text modified from: Neurulation in the normal human embryo. O'Rahilly R, Muller F Ciba Found Symp 1994;181:70-82)
  
 
== Internet Links ==
 
 
* '''Embryo Images'''  [http://www.med.unc.edu/embryo_images/unit-bdyfm/bdyfm_htms/bdyfmtoc.htm Early Cell Populations and Establishment of Body Form] |  [http://www.med.unc.edu/embryo_images/unit-nervous/nerv_htms/nervtoc.htm Nervous System Development]
 
* '''Society for Neuroscience''' [http://web.sfn.org/content/Publications/BrainFacts/index.html http://web.sfn.org/content/Publications/BrainFacts/index.html Brain Facts]
 
* '''Anatomy of the Human Body''' [http://www.bartleby.com/107/7.html The Neural Groove and Tube]
 
* '''Environmental Health Perspectives''' [http://www.ehponline.org/members/2000/suppl-3/511-533rice/rice-full.html Critical Periods of Vulnerability for the Developing Nervous System: Evidence from Humans and Animal Models] | [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1637807 PMC: 1637807] | [http://www.ncbi.nlm.nih.gov/pubmed/10852851 PMID: 10852851]
 
* '''Journal''' [http://www.neuraldevelopment.com/Neural Development]
 
  
 
== References ==
 
== References ==

Revision as of 10:50, 10 August 2010

Notice - Mark Hill
This page will contain the content required when attending the lecture. Currently this page is only a template placeholder and will be updated (this notice removed when completed).

Introduction

The trilaminar embryo

The top layer of the early trilaminar embryo germ layers (ectoderm, mesoderm and endoderm) formed by gastrulation.

Ectoderm Links: Ectoderm, Early Neural, Neural Crest | Lecture 2009 | Category:Ectoderm

Some Recent Findings

File:Neuraltube_001_icon.jpg</wikiflv> This animation shows early neural development from week 3 onward. The whole early embryo development (dorsolateral view) is shown, yolk sac to left.

Epidermis (integumentary, skin contribution) development will be briefly mentioned due to its ectoderm origin, but will also be covered later in the current course.



Stage10 neural sm.jpg Stage10 SEM1.jpg

Ectoderm Development

Stage10 neural sm.jpg
Stage10 SEM1.jpg

Introduction

Note that there are other pages describing neural (central nervous system; brain and spinal cord) and neural crest (peripheral nervous system; sensory and sympathetic ganglia). Epidermis (integumentary, skin contribution) development will be briefly mentioned due to its ectoderm origin.

Objectives

  • Understanding of events during the third and fourth week of development
  • Understanding the process of notochord formation
  • Understanding the process of early neural development
  • Brief understanding of neural crest formation
  • Brief understanding of epidermis formation
  • Understanding of the adult components derived from ectoderm
  • Brief understanding of early neural abnormalities

Textbook References

  • Human Embryology (3rd ed.) Chapter 5 p107-125
  • The Developing Human: Clinically Oriented Embryology (6th ed.)

Other textbooks

  • Moore and Persaud Chapter 18 p451-489
  • Essentials of Human Embryology Larson Chapter 5 p69-79
  • Before We Are Born (5th ed.) Moore and Persaud Chapter 19 p423-458

Development Overview

Notochord

  • forms initially as the Axial Process, a hollow tube which extends from the primitive pit , cranially to the oral membrane
  • the axial process then allow transient communication between the amnion and the yolk sac through the neuroenteric canal.
  • the axial process then merges with the Endodermal layer to form the Notochordal Plate.
  • the notochordal plate then rises back into the Mesodermal layer as a solid column of cells which is the Notochord.

Ectoderm

  • 2 parts
  • midline neural plate
    • columnar
  • lateral surface ectoderm
    • cuboidal
    • sensory placodes
    • epidermis of skin, hair, glands, anterior pituitary, teeth enamel

Neural Plate

Neuralplate cartoon.png
File:Stage11 SEM1.jpg
Stage 11 neural groove to tube
File:Neuralplate_001 icon.jpg</wikiflv> Development of the neural plate region at the embryonic disc stage.

Dorsal view of the embryonic disc from the amniotic cavity side showing the ectoderm with the central region developing into the neural plate.

The neural plate extends from buccopharyngeal membrane to primitive node and forms above the notochord and paraxial mesoderm.The neuroectodermal cells form a broad brain plate and narrower spinal cord region.

Three specific regions medial to lateral can also be identified: midline region floor plate, neural plate, edge of neural plate neural crest



Quicktime version
  • extends from buccopharyngeal membrane to primitive node
  • forms above notochord and paraxial mesoderm
  • neuroectodermal cells
    • broad brain plate
    • narrower spinal cord
  • 3 components form: floor plate, neural plate, neural crest

Neural Determination- neuronal populations are specified before plate folds

  • signals from notochord and mesoderm - secrete noggin, chordin,follistatin
    • all factors bind BMP-4 an inhibitor of neuralation
    • bone morphogenic protein acts through membrane receptor
  • lateral inhibition generates at spinal cord level 3 strips of cells
  • expression of delta inhibits nearby cells, which express notch receptor, from becoming neurons
  • Delta-Notch inetraction- generates Neural strips

Neural Groove

File:Neuraltube_001 icon.jpg</wikiflv> This animation of early neural development from week 3 onward shows the neural groove fusing to form the neural tube.

View - Dorsolateral of the whole early embryo and yolk sac. Cranial (head) to top and caudal (tail) to bottom. Yolk sac is shown to the left.

Beginning with the neural groove initially fusing at the level of the 4th somite to form the neural tube and closing in both directions to leave 2 openings or neuropores: a cranial neuropore (anterior neuropore) and a caudal neuropore (posterior neuropore).

The animation also shows as the embryo grows and folds it increases in size relative to the initial yolk sac. Note also the increasing number of somites over time.

  • forms in the midline of the neural plate (day 18-19)
  • either side of which are the neural folds which continues to deepen until about week 4
  • neural folds begins to fuse, beginning at 4th somite level

Neural Tube

Stage 12 caudal neuropore
  • the neural tube forms the brain and spinal cord
  • fusion of neural groove extends rostrally and caudally
  • begins at the level of 4th somite
  • closes neural groove "zips up" in some species.
    • humans appear to close at multiple points along the tube.
  • leaves 2 openings at either end - Neuropores
    • cranial neuropore closes before caudal

Failure for the neural tube to close correctly or completely results in a neural tube defect.

Secondary Neuralation

File:Secondary_neurulation_01 icon.jpg</wikiflv> This animation shows the early developmental process often described as secondary neurulation.

Red - site of secondary neurulation | Blue - neural tube

  • caudal end of neural tube formed by secondary neuralation
  • develops from primitive streak region
  • solid cord canalized by extension of neural canal
  • mesodermal caudal eminence
Links: Quicktime version | Neural System Development


Neural Crest

Neural Crest Notes

  • a population of cells at the edge of the neural plate that lie dorsally when the neural tube fuses
    • dorsal to the neural tube, as a pair of streaks
    • pluripotential, forms many different types of cells
    • cells migrate throughout the embryo
    • studied by quail-chick chimeras
    • transplanted quail cells have obvious nucleoli compared with chicken

Neural Crest Derivitives

  • dorsal root ganglia
  • autonomic ganglia
  • adrenal medulla
  • drg sheath cells, glia
  • pia-arachnoid sheath
  • skin melanocytes
  • connective tissue of cardiac outflow
  • thyroid parafollicular cells
  • craniofacial skeleton
  • teeth odontoblasts


Ectodermal Placodes

  • Specialized ectodermal "patches" in the head region
  • Contribute sensory structures - otic placode (otocyst), nasal placode, lens placode
  • Contribute teeth

Human Neuralation - Early Stages

The stages below refer to specific Carneigie stages of development.

  • stage 8 (about 18 postovulatory days) neural groove and folds are first seen
  • stage 9 the three main divisions of the brain, which are not cerebral vesicles, can be distinguished while the neural groove is still completely open. Stage 9 SEM
  • stage 10 (two days later) neural folds begin to fuse near the junction between brain and spinal cord, when neural crest cells are arising mainly from the neural ectoderm Stage 10 SEM
  • stage 11 (about 24 days) the rostral (or cephalic) neuropore closes within a few hours; closure is bidirectional, it takes place from the dorsal and terminal lips and may occur in several areas simultaneously. The two lips, however, behave differently. Stage 11 SEM
  • stage 12 (about 26 days) The caudal neuropore takes a day to close Stage 12 SEM
  • the level of final closure is approximately at future somitic pair 31
  • corresponds to the level of sacral vertebra 2
  • stage 13 (4 weeks) the neural tube is normally completely closed Stage 13 SEM

Secondary neurulation begins at stage 12 - is the differentiation of the caudal part of the neural tube from the caudal eminence (or end-bud) without the intermediate phase of a neural plate.

(Stage text modified from: Neurulation in the normal human embryo. O'Rahilly R, Muller F Ciba Found Symp 1994;181:70-82)


References

Textbooks

  • The Developing Human: Clinically Oriented Embryology (8th Edition) by Keith L. Moore and T.V.N Persaud - Mesoderm Ch15,16: p405-423, 426-430 Body Cavities Ch9: p174-184
  • Larsen’s Human Embryology by GC. Schoenwolf, SB. Bleyl, PR. Brauer and PH. Francis-West - Mesoderm Ch11 p311-339 Body Cavities Ch6 p127-146

Additional Textbooks

  • Before We Are Born (5th ed.) Moore and Persaud Ch16,17: p379-397, 399-405
  • Essentials of Human Embryology Larson Ch11 p207-228
  • Human Embryology Fitzgerald and Fitzgerald Body Cavities Ch5 p29-32, Ch7 p47,48
  • Human Embryology and Developmental Biology ?Carlson Ch9,10: p173-193, 209-222 Body Cavities Ch5 p29-32, Ch7 p47,48

Online Textbooks

Search

Reviews

  • Temporal dynamics of patterning by morphogen gradients. Kutejova E, Briscoe J, Kicheva A. Curr Opin Genet Dev. 2009 Jul 9. PMID: 19596567
  • The Hedgehog, TGF-beta/BMP and Wnt families of morphogens in axon guidance. Charron F, Tessier-Lavigne M. Adv Exp Med Biol. 2007;621:116-33. Review.

PMID: 18269215

  • Novel brain wiring functions for classical morphogens: a role as graded positional cues in axon guidance. Charron F, Tessier-Lavigne M. Development. 2005 May;132(10):2251-62. Review.

PMID: 15857918 | Development Link

Movies

Neuralplate 001 icon.jpg Neuraltube 001 icon.jpg
Neural Plate Neural Tube

References


Reviews

Articles

Historic

Search PubMed

Search NLM Online Textbooks: "Ectoderm" : Developmental Biology | The Cell- A molecular Approach | Molecular Biology of the Cell


Search Pubmed: Ectoderm

Take the Quiz

1

Ectoderm refers only to the neural plate region of the trilaminar embryo

true
false

2

The central nervous system forms in the sequence:

norochord to neural plate to neural tube
neural tube to neural plate to neural groove
neural plate to neural groove to neural tube
neural plate to neural crest to neural zone

3

The neural plate is narrower at the caudal (tail) end and therefore closes earlier than the broad cranial (head) end.

true
false

4

The correct sequence from cranial to caudal of the secondary brain vesicles is:

prosencephalon, mesencephalon, metencephalon, myelencephalon, rhombencephalon
telencephalon, diencephalon, metencephalon, mesencephalon, myelencephalon
telencephalon, diencephalon, mesencephalon, metencephalon, myelencephalon
prosencephalon, diencephalon, mesencephalon, myelencephalon, metencephalon

Glossary Links

Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link

Cite this page: Hill, M.A. (2019, October 18) Embryology Ectoderm. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Ectoderm

What Links Here?
© Dr Mark Hill 2019, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G