User:Z3414648
Welcome to the 2014 Embryology Course!
- Links: Timetable | How to work online | One page Wiki Reference Card | Moodle
- Each week the individual assessment questions will be displayed in the practical class pages and also added here.
- Copy the assessment items to your own page and provide your answer.
- Note - Some guest assessments may require completion of a worksheet that will be handed in in class with your student name and ID.
Individual Lab Assessment |
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Lab 12 - Stem Cell Presentation Assessment | More Info | |
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Group | Comment | Mark (10) |
1/8 |
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7 |
2 |
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7.5 |
3 |
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7.5 |
4 |
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8.5 |
5 |
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8.5 |
6 |
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8.5 |
7 |
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7.5 |
<pubmed>24934154</pubmed>
This article from PubMed explores the role of the ZP2 receptor and protein in the prevention of polyspermy and ensuring only species-specific fusion of spermatozoa and an oocyte occurs. ZP2 is a glycoprotein found in a region of the extracellular oocyte-surrounding zona pellucida . Polyspermy is an abnormal and detrimental process where membrane fusion occurs between one oocyte and more than one spermatozoa. It is inefficient for more than one spermatozoa to bind to the oocyte because only one male and female gamete are required for successful fertilisation. The authors of this article used gamete samples from both mice and human origin to illustrate their findings.
The authors used various materials and scientific methods in order to achieve their results. There were extensive, complex and repetitive biochemical and transgenic variations made in order to manipulate the gene expression and protein synthesis occurring in the test subjects, in this case mice. In order to control which cells could be genetically modified to express human or mice ZP2 protein, they scientists needed to first produce transgenic mouse lines from embryonic stem cells that had not yet differentiated into any of the ZP1, ZP2, ZP3 or ZP4. They created Bacterial Artifical Chromosomes carrying either the mouse or human form of the ZP2 gene and these were transformed into bacterial cells containing the gamma prophage. The recombinants could be identified by growing the transformed cells on minimal media with galactose. Once the transgenes were inserted into surrogate mothers, the mice could be
<pubmed>23909991</pubmed>