User:Z3417796
Welcome to the 2014 Embryology Course!
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- Each week the individual assessment questions will be displayed in the practical class pages and also added here.
- Copy the assessment items to your own page and provide your answer.
- Note - Some guest assessments may require completion of a worksheet that will be handed in in class with your student name and ID.
Individual Lab Assessment |
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Lab 12 - Stem Cell Presentation Assessment | More Info | |
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Group | Comment | Mark (10) |
1/8 |
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7 |
2 |
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7.5 |
3 |
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7.5 |
4 |
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8.5 |
5 |
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8.5 |
6 |
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8.5 |
7 |
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7.5 |
Lab Attendance
Lab 1--Z3417796 (talk) 12:52, 6 August 2014 (EST)
Practice
Links
http://www.ncbi.nlm.nih.gov/pubmed [1]
Reference
http://www.ncbi.nlm.nih.gov/pubmed/25084016 [2] <pubmed>25084016</pubmed>
Individual Assessments
Lab 1
Reference 1
http://www.ncbi.nlm.nih.gov/pubmed/23835722 [3] <pubmed>23835722</pubmed>
Purpose
The usefulness of low O2 concentrations in human IVF technology is an ongoing question with numerous laboratories still uncertain as to the actual influence and significance on clinical outcome. The purpose of this randomised clinical trial was to investigate the impact of atmospheric vs. low concentrations of oxygen (O2) during the complete process of human zygote and embryonic development. The study was performed utilising sibling oocytes with the differentiations between the two O2 culture levels measured from fertilisation, from embryo until blastocyst formation, through pregnancy and live birth.
Method
The participants of the study comprised of 258 women who underwent intracytoplasmic sperm injection (ICSI) treatment with a minimum of eight oocytes extracted. The recovered oocytes were cultured and randomly allocated into one of two-treatment groups- incubation in either 5% or 20% O2 conditions. The temperature in both incubators was 37 °C. Evaluation of embryonic development was made in terms of fertilisation, cleavage and the quality of both the embryo and blastocyst. Secondary factors assessed included implantation, maintenance of pregnancy and live births.
Results
A sum of 3,638 matured (metaphase II phase) oocytes were extracted through the study, of which 1833 were incubated under 5% O2 conditions and 1805 sibling oocytes under the alternate 20% O2 treatment condition. Levels of fertilisation and rates of cleavage between the two treatment groups showed no significant differences. However, significant distinctions were seen with the 5% O2 group, which presented significantly more blastomeres (p<0.05), a greater quantity of high quality day 3 embryos (p<0.02) in addition to a significantly increased number of available embryos, per cycle, for transfer and freezing (31.6% vs. 23.1% for the 20% O2 group; P<0.0001). The lower oxygen concentration also seemed to suggest a better influence on clinical outcomes, with significantly higher rates of implantation, pregnancy and live births (22.1% vs. 10.3%, P<0.03; 38.2% vs.18.4%, P<0.05, 34.2% vs. 15.8%, P<0.05 respectively).