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===Article 2=== | ===Article 2=== | ||
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879877/ PMID3879877] | [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3879877/ PMID3879877] | ||
Seminal plasma concentrations of pH, total Calcium, ionized calcium, and inorganic phosphate were recorded in 80 male patients to find a correlation of the substances to mobility and spermatazoa count. The 31 patients who were recorded as having hypomotility (<60%) exhibited lower calcium concentrations (0.19+0.01mmol/L) compared with the normal motility group ( 0.24+0.01mmol/L). The same was observed with phosphate levels (hypo= 5.64+1.62mmol/L normal= 7.83+1.27 ). No noticable differences were observed in the pH levels betweeen the two groups. Of those in the hypomotile group, there was a greater occurance of abnormal form in the spermatazoa, 36% compared to the normal groups 5%. The mobility and count of the spermatazoa was performed using a binocular microscope and improved neubauer counting chamber. | |||
The article shows that there was a relationship between calcium and phosphate levels that indicate that lower levels of the two in seminal fluid would result in lower count and motility as well as presence of abnormal forms in the spermatazoa. There did seem to be a paradoxical effect from calcium levels on sperm depending the maturation level of the sperm. In the epididymis, calcium ion stimulate immature sperm whereas in ejaculated semen, it inhibits sperm motility. | |||
<<pubmed>PMC3879877</pubmed>> | <<pubmed>PMC3879877</pubmed>> | ||
====method & findings==== | ====method & findings==== |
Revision as of 09:44, 13 August 2014
Welcome to the 2014 Embryology Course!
- Links: Timetable | How to work online | One page Wiki Reference Card | Moodle
- Each week the individual assessment questions will be displayed in the practical class pages and also added here.
- Copy the assessment items to your own page and provide your answer.
- Note - Some guest assessments may require completion of a worksheet that will be handed in in class with your student name and ID.
Individual Lab Assessment |
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Lab 12 - Stem Cell Presentation Assessment | More Info | |
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Group | Comment | Mark (10) |
1/8 |
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7 |
2 |
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7.5 |
3 |
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7.5 |
4 |
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8.5 |
5 |
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8.5 |
6 |
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8.5 |
7 |
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7.5 |
Online Assessment
Lab 1
Article 1
<<pubmed>24520460</pubmed>>
method & findings
women aged 20 to 29 with homogenous characteristics of basal hormone levels, duration of infertility, Body mass index, antral follicle count and age were split into 4 groups of differing endometrial wall thickness (1 <7mm, 2 7-10mm, 3 10-14, 4 >14mm). thickness was measure using TV- USG in the midsagittal plane on the day of hCG administration when 1 or 2 follicles reached 17mm in size. 35-36 hours following hCG for final maturation, TV- USGguided needle aspiration of the follicular fluid was carried out, as was ICSI in all cases. Luteal phase was supported on the oocyte "pick-up" day until serum pregnancy test 12 days later. Clinical pregnancy could be confirmed by presence of fetal sac or fetal cardiac activity 2 weeks later via ultrasound.
The article found that of the women in group 1 (endometrial wall = <7mm) there was a dramatic reduction in Implantation rate, CPR, and ongoing pregnancy rate (OPR)compared to group 2, 3 and 4. There didnt seem to be a significant difference between 2, 3, and 4 in this catagory of result. Retrieved oocyte number, transferred embryo number, and the fertilization, cleavage, and implantation rates (IR) was also found to be similar in all four groups. Results then showed that women of an endometrial wall thickness of less than 7mm would experience a significantly lower clinical pregnancy rate (although no threshold was observed)
Article 2
PMID3879877 Seminal plasma concentrations of pH, total Calcium, ionized calcium, and inorganic phosphate were recorded in 80 male patients to find a correlation of the substances to mobility and spermatazoa count. The 31 patients who were recorded as having hypomotility (<60%) exhibited lower calcium concentrations (0.19+0.01mmol/L) compared with the normal motility group ( 0.24+0.01mmol/L). The same was observed with phosphate levels (hypo= 5.64+1.62mmol/L normal= 7.83+1.27 ). No noticable differences were observed in the pH levels betweeen the two groups. Of those in the hypomotile group, there was a greater occurance of abnormal form in the spermatazoa, 36% compared to the normal groups 5%. The mobility and count of the spermatazoa was performed using a binocular microscope and improved neubauer counting chamber.
The article shows that there was a relationship between calcium and phosphate levels that indicate that lower levels of the two in seminal fluid would result in lower count and motility as well as presence of abnormal forms in the spermatazoa. There did seem to be a paradoxical effect from calcium levels on sperm depending the maturation level of the sperm. In the epididymis, calcium ion stimulate immature sperm whereas in ejaculated semen, it inhibits sperm motility. <<pubmed>PMC3879877</pubmed>>
method & findings
Lab Attendance