2010 Lab 5: Difference between revisions
Line 399: | Line 399: | ||
[[:File:Stage_22 image 098.jpg|G7L]] | [[:File:Stage_22 image 098.jpg|G7L]] | ||
| Anal canal with triangular lumen. | | Anal canal with triangular lumen. | ||
|} | |||
== Respiratory - Mid-Embryonic (Stage 13)== | |||
{| | |||
| [[File:Stage14 respiratory tract.jpg]] | |||
| [[File:Stage13-GIT-icon.jpg|150px|link=Movie_-_Gastrointestinal_Tract_3D_stage_13]] | |||
[[Movie_-_Gastrointestinal_Tract_3D_stage_13|Respiratory]] | |||
| The individual serial slices have also been incorporated into a 3D model of this embryo. | |||
The table below shows a thumbnail view of each section and a brief description of related respiratory components. | |||
|} | |} | ||
{| | :{{Template:Respiratory Links}} | [[Carnegie stage 13]] | ||
|+ | |||
{| class="wikitable" border="1" | |||
|+ [[Carnegie stage 13 - serial sections|Stage 13]] '''- Respiratory''' | |||
! Section | |||
! Name | |||
! Description | |||
|- | |- | ||
| [[ | | width= 210px|[[File:Stage 13 image 057.jpg|200px]] | ||
[[File:Stage 13 image 058.jpg|200px]] | |||
| [[:File: | |||
| width= 30px|[[:File:Stage 13 image 057.jpg|B1L]] | |||
| [[ | |||
[[:File:Stage 13 image 058.jpg|B2L]] | |||
| Pharynx. | |||
Crest in ventral floor of [[P#pharynx|pharynx]] formed by fusion of 3rd pharyngeal arches = [[H#hypopharyngeal eminence|hypopharyngeal eminence]] (precursor of root of tongue). | |||
Rathke's pouch forming the rudimentary [[A#adenohypophysis|adenohypophysis]] (anterior pituitary). | |||
|- | |- | ||
| [[File: | | [[File:Stage 13 image 059.jpg|200px]] | ||
| [[ | | [[:File:Stage 13 image 059.jpg|B3L]] | ||
| Rudimentary thyroid ventral to aortic sac (also seen in [[:File:Stage 13 image 058.jpg|B2]], ventral to the hypopharyngeal eminence). | |||
| [[ | |||
|- | |- | ||
| [[ | | [[File:Stage 13 image 060.jpg|200px]] | ||
| [[:File:Stage 13 image 060.jpg|B4L]] | |||
| Caudal pharynx compressed dorsoventrally. | |||
| [[:File: | |||
| | |||
|- | |- | ||
| [[File: | | [[File:Stage 13 image 063.jpg|200px]] | ||
| [[ | | [[:File:Stage 13 image 063.jpg|B7L]] | ||
| Glottis drawn off from pharyngeal foregut. | |||
| | |||
Nasal placodes. Pulmonary arteries. | |||
|- | |- | ||
| [[ | | [[File:Stage 13 image 064.jpg|200px]] | ||
[[File:Stage 13 image 065.jpg|200px]] | |||
| [[:File: | | [[:File:Stage 13 image 064.jpg|C1L]] | ||
| | |||
[[:File:Stage 13 image 065.jpg|C2L]] | |||
| Commencement of trachea and oesophagus with dense mesenchyme. | |||
Right nasal pit. | |||
|- | |- | ||
| [[File: | | [[File:Stage 13 image 066.jpg|200px]] | ||
[[File:Stage 13 image 067.jpg|200px]] | |||
| [[File: | | [[:File:Stage 13 image 066.jpg|C3L]] | ||
| [[ | |||
[[:File:Stage 13 image 067.jpg|C4L]] | |||
| Common cardinal vein in the posterior wall of the [[I#intraembryonic coelom|intraembryonic coelom]]. | |||
The pleuropericardial folds which contribute later to the formation of the pleura and pericardium. | |||
In [[:File:Stage 13 image 067.jpg|C4]], junction of right common cardinal vein with dorsal wall of sinus venosus. | |||
Left nasal pit. | |||
|- | |- | ||
| [[ | | [[File:Stage 13 image 068.jpg|200px]] | ||
| [[:File: | | [[:File:Stage 13 image 068.jpg|C5L]] | ||
| | | Smaller oesophagus, expanding trachea. Note ventral anchoring of attachment site is at the most cranial extension of the septum transversum. | ||
Note also that this attachment now divides the intraembryonic coelom around the trachea into two canals, the L and R pleuro (pericardio-peritoneal) canals. (Canals are lined by coelomic mesothelium and are continuous with whole I-E coelom - they will be referred to hereafter simply as coelomic canals). | |||
Note the pleuroperitoneal fold on the medial side of the R common cardinal vein - this fold will form part of the diaphragm. | |||
|- | |- | ||
| [[File: | | [[File:Stage 13 image 068.jpg|200px]] | ||
[[File:Stage 13 image 069.jpg|200px]] | |||
| [[File: | | [[:File:Stage 13 image 068.jpg|C5L]] | ||
| | |||
[[:File:Stage 13 image 069.jpg|C6L]] | |||
| Lateral extension of pulmonary mesenchyme is moulded to shape of coelomic canals. Oesophagus lumen obliterated (common site of oesophageal atresia and/or tracheo-oesophageal fistula). Prominent R pleuroperitoneal fold. | |||
|- | |- | ||
| [[ | | [[File:Stage 13 image 070.jpg|200px]] | ||
| [[:File: | | [[:File:Stage 13 image 070.jpg|C7L]] | ||
| | | Note dorsal extent of coelomic canals. | ||
Oesophagus lumen reappears caudal to bifurcation. | |||
Distinct R (smaller on L) pleuroperitoneal fold below the common cardinal vein. | |||
|- | |- | ||
| [[File: | | [[File:Stage 13 image 071.jpg|200px]] | ||
| [[ | | [[:File:Stage 13 image 071.jpg|D1L]] | ||
| Oesophagus/stomach junction. Coelomic canals. | |||
| | |||
|- | |- | ||
| [[ | | [[File:Stage 13 image 072.jpg|200px]] | ||
| [[:File: | | [[:File:Stage 13 image 072.jpg|D2L]] | ||
| | | Ovoid stomach with developing space of the lesser sac on R. | ||
Dorsal and ventral attachments of the mesenchyme are now known as dorsal and ventral mesogastria. Coelomic canals. | |||
|- | |- | ||
| [[File: | | [[File:Stage 13 image 098.jpg|200px]] | ||
| [[ | | [[:File:Stage 13 image 098.jpg|G7L]] | ||
| Caudal pharynx (extending laterally, ventral to dorsal aorta - cf B4). Stomach, mesentery | |||
| | |||
|- | |- | ||
| [[ | | [[File:Stage 13 image 097.jpg|200px]] | ||
| [[:File: | | [[:File:Stage 13 image 097.jpg|G6L]] | ||
| Narrow oesophagus. Tracheal bifurcation dorsal to sinus venosus. | |||
| | |||
|} | |} |
Revision as of 14:29, 25 August 2010
Introduction
This laboratory will allow time to study both gastrointestinal tract and respiratory development. The class will study features and events of development occurring: early-embryonic, mid-embryonic, late-embryonic and fetal.
Gastrointestinal Tract Movies
Endoderm | Yolk Sac | Tract Growth | Stomach Rotation | Lesser Sac | Greater Omentum |
Quicktime version | Quicktime version | Quicktime version | Quicktime version | Quicktime version | Quicktime version |
Gastrointestinal Tract - Mid-Embryonic (Stage 13)
The individual serial slices have also been incorporated into a 3D model of this embryo.
Section | Name | Description |
---|---|---|
B1L | Pharynx.
Crest in ventral floor of pharynx formed by fusion of 3rd pharyngeal arches = hypopharyngeal eminence (precursor of root of tongue). Rathke's pouch forming the rudimentary adenohypophysis (anterior pituitary). | |
B3L | Rudimentary thyroid ventral to aortic sac (also seen in B2, ventral to the hypopharyngeal eminence). | |
B4L | Caudal pharynx compressed dorsoventrally. | |
B7L | Glottis drawn off from pharyngeal foregut.
Nasal placodes. Pulmonary arteries. | |
C1L | Commencement of trachea and oesophagus with dense mesenchyme.
Right nasal pit. | |
C3L | Common cardinal vein in the posterior wall of the intraembryonic coelom.
The pleuropericardial folds which contribute later to the formation of the pleura and pericardium. In C4, junction of right common cardinal vein with dorsal wall of sinus venosus. Left nasal pit. | |
C5L | Smaller oesophagus, expanding trachea. Note ventral anchoring of attachment site is at the most cranial extension of the septum transversum.
Note also that this attachment now divides the intraembryonic coelom around the trachea into two canals, the L and R pleuro (pericardio-peritoneal) canals. (Canals are lined by coelomic mesothelium and are continuous with whole I-E coelom - they will be referred to hereafter simply as coelomic canals). Note the pleuroperitoneal fold on the medial side of the R common cardinal vein - this fold will form part of the diaphragm. | |
C5L | Lateral extension of pulmonary mesenchyme is moulded to shape of coelomic canals. Oesophagus lumen obliterated (common site of oesophageal atresia and/or tracheo-oesophageal fistula). Prominent R pleuroperitoneal fold. | |
C7L | Note dorsal extent of coelomic canals.
Oesophagus lumen reappears caudal to bifurcation. Distinct R (smaller on L) pleuroperitoneal fold below the common cardinal vein. | |
D1L | Oesophagus/stomach junction. Coelomic canals. | |
D2L | Ovoid stomach with developing space of the lesser sac on R.
Dorsal and ventral attachments of the mesenchyme are now known as dorsal and ventral mesogastria. Coelomic canals. | |
D3L | Rotation of stomach (seen from above) to right side.
Note change in outline of coelomic canals due to presence of liver. Lesser sac. Note thick mesothelium lining the coelom along left edge of stomach, the primordium of the spleen and greater omentum along greater curvature. Liver embedded in septum transversum (ventral border of septum transversum contributes to diaphragm). | |
G7L | Caudal pharynx (extending laterally, ventral to dorsal aorta - cf B4). Stomach, mesentery | |
G6L | Narrow oesophagus. Tracheal bifurcation dorsal to sinus venosus. |
All Stage 13 Sections
A1L | A2L | A3L | A4L | A5L | A6L | A7L |
B1L | B2L | B3L | B4L | B5L | B6L | B7L |
C1L | C2L | C3L | C4L | C5L | C6L | C7L |
D1L | D2L | D3L | D4L | D5L | D6L | D7L |
E1L | E2L | E3L | E4L | E5L | E6L | E7L |
F1L | F2L | F3L | F4L | F5L | F6L | F7L |
G1L | G2L | G3L | G4L | G5L | G6L | G7L |
Gastrointestinal Tract - Late-Embryonic (Stage 22)
The individual serial slices have also been incorporated into a 3D model of this embryo.
Section | Name | Description |
---|---|---|
E6L | Liver. Ductus venosus.
Cardio-oesophageal junction (cf. E5). Inferior vena cava. | |
E7L | Stomach body, with mucosa, submucosa and muscularis externa.
Lesser sac. Lesser omentum. Pyloroduodenal junction. Folded duodenal mucosa. Inferior vena cava. Portal vein. Hepatic ducts. Gallbladder. | |
F1L | Stomach body. Spleen. Pyloric canal. Duodenum.
Pancreas. Small intestine loop (jejunum) cut tangentially, ventral to liver. Portal vein. | |
F2L | Stomach, spleen. Superior mesenteric artery.
Superior mesenteric vein crossing cranial to body of pancreas. Tail of pancreas. Duodenum. Small intestinal loop herniating from abdominal cavity into the coelom of the umbilical cord (remnant of extra-embryonic coelom). | |
F4L | Greater curvature of stomach (tangential section). Lesser sac. Greater omentum. Duodenal/jejunal junction.
Note colon (small lumen, darkly-staining wall) and its mesocolon. Note the sections of small and large intestine within the umbilical cord coelom and their mesenteries. Note the thickened jelly to one side of the umbilical cord, containing umbilical vein and R umbilical artery. | |
F5L | Lesser sac. Greater omentum. Duodenum. Jejunum (cut twice with mesentery in between). Colon and mesocolon. | |
F6L | Greater omentum and lesser sac.
Jejunum with mesentery. Colon with mesocolon. Three layers of abdominal muscles. Both umbilical arteries now inside abdominal cavity with urachus between them. | |
F7L | In abdominal cavity - colon with mesocolon, jejunum. Greater omentum and lesser sac.
Umbilical cord - containing umbilical arteries and small dark allantois. Umbilical cord coelom containing mainly, small intestinal loops with their mesentery. | |
G1L
|
Umbilical cord and coelom containing small intestine loops.
Colon and mesocolon. Jejunum (G1 only). Bladder with umbilical arteries either side. Knees. | |
G3L | Rectum.
Bladder. Umbilical arteries arising from common iliac arteries. | |
G4L | Rectum. | |
G5L | Recto-anal junction with rectovesical pouch of peritoneal cavity. | |
G6L | Anal canal with triangular lumen. |
Respiratory - Mid-Embryonic (Stage 13)
Section | Name | Description |
---|---|---|
B1L | Pharynx.
Crest in ventral floor of pharynx formed by fusion of 3rd pharyngeal arches = hypopharyngeal eminence (precursor of root of tongue). Rathke's pouch forming the rudimentary adenohypophysis (anterior pituitary). | |
B3L | Rudimentary thyroid ventral to aortic sac (also seen in B2, ventral to the hypopharyngeal eminence). | |
B4L | Caudal pharynx compressed dorsoventrally. | |
B7L | Glottis drawn off from pharyngeal foregut.
Nasal placodes. Pulmonary arteries. | |
C1L | Commencement of trachea and oesophagus with dense mesenchyme.
Right nasal pit. | |
C3L | Common cardinal vein in the posterior wall of the intraembryonic coelom.
The pleuropericardial folds which contribute later to the formation of the pleura and pericardium. In C4, junction of right common cardinal vein with dorsal wall of sinus venosus. Left nasal pit. | |
C5L | Smaller oesophagus, expanding trachea. Note ventral anchoring of attachment site is at the most cranial extension of the septum transversum.
Note also that this attachment now divides the intraembryonic coelom around the trachea into two canals, the L and R pleuro (pericardio-peritoneal) canals. (Canals are lined by coelomic mesothelium and are continuous with whole I-E coelom - they will be referred to hereafter simply as coelomic canals). Note the pleuroperitoneal fold on the medial side of the R common cardinal vein - this fold will form part of the diaphragm. | |
C5L | Lateral extension of pulmonary mesenchyme is moulded to shape of coelomic canals. Oesophagus lumen obliterated (common site of oesophageal atresia and/or tracheo-oesophageal fistula). Prominent R pleuroperitoneal fold. | |
C7L | Note dorsal extent of coelomic canals.
Oesophagus lumen reappears caudal to bifurcation. Distinct R (smaller on L) pleuroperitoneal fold below the common cardinal vein. | |
D1L | Oesophagus/stomach junction. Coelomic canals. | |
D2L | Ovoid stomach with developing space of the lesser sac on R.
Dorsal and ventral attachments of the mesenchyme are now known as dorsal and ventral mesogastria. Coelomic canals. | |
G7L | Caudal pharynx (extending laterally, ventral to dorsal aorta - cf B4). Stomach, mesentery | |
G6L | Narrow oesophagus. Tracheal bifurcation dorsal to sinus venosus. |