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However, the results concluded that IVF culture at 36 degrees does not improve the conditions for blastulation and pregnancy rates in human in IVF. Thus, maintaining the existing temperature or changing it to 26 degrees does not alter the effects or success of IVF. | However, the results concluded that IVF culture at 36 degrees does not improve the conditions for blastulation and pregnancy rates in human in IVF. Thus, maintaining the existing temperature or changing it to 26 degrees does not alter the effects or success of IVF. | ||
==Lab Assessment 2== |
Revision as of 21:30, 19 August 2014
Welcome to the 2014 Embryology Course!
- Links: Timetable | How to work online | One page Wiki Reference Card | Moodle
- Each week the individual assessment questions will be displayed in the practical class pages and also added here.
- Copy the assessment items to your own page and provide your answer.
- Note - Some guest assessments may require completion of a worksheet that will be handed in in class with your student name and ID.
Individual Lab Assessment |
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Lab 12 - Stem Cell Presentation Assessment | More Info | |
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Group | Comment | Mark (10) |
1/8 |
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7 |
2 |
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7.5 |
3 |
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7.5 |
4 |
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8.5 |
5 |
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8.5 |
6 |
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8.5 |
7 |
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7.5 |
Lab Attendance
Lab1 --Z3332339 (talk) 12:45, 6 August 2014 (EST)
Lab2 --Z3332339 (talk) 11:13, 13 August 2014 (EST)
http://www.ncbi.nlm.nih.gov/pubmed
<pubmed>25084016</pubmed>
Lab Assessment 1
A role for carbohydrate recognition in mammalian sperm-egg binding The primary focus of this article is on the first stage of fertilization, the binding of sperm to the specialised extracellular matrix of the egg, known as the zona pelluicda (ZP). The article suggests that the mammalian egg cell has a specialised carbohydrate site on the ZP for which the sperm recognises and binds to, enabling the fusion of genetic information between these two gametes.
The article explains how it was previously thought that data obtained from mouse sperm-egg interactions could explain human sperm-cell binding. However, recent research has suggested that the mouse model cannot be directly applied to the human model. Thus, this research paper investigates sperm-ZP interactions, using humans as the predominant model in finding the specific requirements for human sperm-egg binding which couldn’t previously be explained by the mouse model.
This article also uses a review that focused on the identification of the egg binding proteins associated with the binding of human sperm to the egg. Their findings concluded identifying the role for carbohydrate recognition on the ZP. These carbohydrates have specific sequences that cause restriction of ZP glycosylation in humans that could not otherwise be explained in mouse and pig models or are not the same for humans. This finding suggests that the regulation of glycosylation could be directly correlated with the degree of organismal complexity. Evidence favouring this concept would require the sequencing of ZP glycoproteins from other mammals at different levels of the evolutionary ladder, which could be are areas of future directions for this research.
Examining the temperature of embryo culture in in vitro fertilization: a randomized controlled trial comparing traditional core temperature (37°C) to a more physiologic, cooler temperature (36°C)
The study undertaken in this article was to determine if better clinical outcomes of IVF resulted from embryo cultures in cooler temperatures (36 degrees) as oppose to the traditional core temperature of (37 degrees).
The method of investigation: retrieving eight or more oocytes from a female of 42 years of age, with infertile couples (n=52). These mature oocytes were divided into two groups to be cultured at different temperatures; one group at 36 degrees, the other at 37 degrees. The rate of development and expansion of blastocysts (volume), fertilization, aneuploidy and sustained implantation were the factors measured to in order to determine which of these conditions clinically improved the environment best for embryonic development. This could potentially change the temperatures of which in vitro fertilization takes places in clinics in the future.
However, the results concluded that IVF culture at 36 degrees does not improve the conditions for blastulation and pregnancy rates in human in IVF. Thus, maintaining the existing temperature or changing it to 26 degrees does not alter the effects or success of IVF.