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Ovulated eggs from human ZP1 (huZP1), huZP2, and huZP3 rescue as well as huZP4 transgenic mice were fixed and stained with mAb to human ZP1 (huZP1), huZP2, huZP3, huZP4, mouse ZP1 (moZP1), moZP2, and moZP3. Antibody binding to human and mouse zona proteins was detected by confocal microscopy and faux colored dark blue (huZP1), red (huZP2), green (huZP3), orange (huZP4), magenta (moZP1), light blue (moZP2), or yellow (moZP3). Fluorescent and DIC images were merged. | Ovulated eggs from human ZP1 (huZP1), huZP2, and huZP3 rescue as well as huZP4 transgenic mice were fixed and stained with mAb to human ZP1 (huZP1), huZP2, huZP3, huZP4, mouse ZP1 (moZP1), moZP2, and moZP3. Antibody binding to human and mouse zona proteins was detected by confocal microscopy and faux colored dark blue (huZP1), red (huZP2), green (huZP3), orange (huZP4), magenta (moZP1), light blue (moZP2), or yellow (moZP3). Fluorescent and DIC images were merged. | ||
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===Reference=== | |||
<pubmed>22734000</pubmed>| [http://jcb.rupress.org/content/197/7/897.long J Cell Biol.] | |||
{{JCB}} | {{JCB}} | ||
[[Category:Oocyte]][[Category:Zona pellucida]] |
Revision as of 16:03, 4 August 2014
Human zona proteins incorporated into transgenic mouse oocyte zona pellucida
Ovulated eggs from human ZP1 (huZP1), huZP2, and huZP3 rescue as well as huZP4 transgenic mice were fixed and stained with mAb to human ZP1 (huZP1), huZP2, huZP3, huZP4, mouse ZP1 (moZP1), moZP2, and moZP3. Antibody binding to human and mouse zona proteins was detected by confocal microscopy and faux colored dark blue (huZP1), red (huZP2), green (huZP3), orange (huZP4), magenta (moZP1), light blue (moZP2), or yellow (moZP3). Fluorescent and DIC images were merged.
Reference
<pubmed>22734000</pubmed>| J Cell Biol.
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current | 16:00, 4 August 2014 | ![]() | 1,280 × 717 (244 KB) | Z8600021 (talk | contribs) | ==Human zona proteins incorporated into transgenic mouse oocyte zona pellucida== Ovulated eggs from human ZP1 (huZP1), huZP2, and huZP3 rescue as well as huZP4 transgenic mice were fixed and stained with mAb to human ZP1 (huZP1), huZP2, huZP3, huZP4,... |
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