File:Human Carnegie stage 13 SOX11 MAZ GJA1 expression.jpg

In situ expression of SOX11, MAZ and GJA1 in the human embryo.

(A) Hematoxylin-eosin (HE) stain of caudal trunk-level transverse section at stage Carnegie (C)13.

(B) Adjacent section. Presumptive dermatome, neural tube excluding floorplate, pronephros and splanchnopleural mesoderm and neural crest cells (arrowheads) express SOX11.

(C) The same tissues express GJA1 in a simultaneously hybridized adjacent section. Expression is higher in the dermatome, dorsal neural tube and migratory neural crest cells (arrowheads).

(D) MAZ is expressed in an adjacent section in most of the neural tube aside from the floorplate (indicated as extension of dotted lines), and lightly in neural crest (arrowheads), dermamyotome and pronephros.

(E) Forelimb-level HE transverse section in separate C13 embryo.

(F) GJA1 antisense probe-hybridized adjacent section. Limb bud, dorsal neural tube and dorsal roots (arrowheads) hybridize more strongly than other tissues, nearly all of which have some basal GJA1 expression.

(G and J) GJA1 sense probe-hybridized adjacent section to previous frame, demonstrating specificity of antisense hybridizations.

(H) Rostral trunk-level HE section of same embryo as in (A–D).

(I) GJA1 is highly expressed in the dorsal neural tube, the dorsal roots and ganglia, the dermatome and the splanchnopleural mesoderm, with basal expression in all other tissues of the section as compared with

GJA1 - gap junction protein, alpha 1, 43kDa encoding connexin43, which is one of 21 connexin proteins in humans. http://ghr.nlm.nih.gov/gene/GJA1

Abbreviations:

Bar = 250 µm.

Original File: Figure 4. http://hmg.oxfordjournals.org/cgi/content/full/17/21/3411/DDN235F4

Links: Carnegie stage 13 | Neural Crest Development

Reference

<pubmed>18689800</pubmed>| Hum Mol Genet.

© 2008 The Author(s)

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