User:Z3377257: Difference between revisions

Revision as of 11:03, 31 July 2014

Introduction

HLA-G

OMIM 142871 - HLAG HLA-6.0; HLA60, T-CELL A LOCUS; TCA
- Human leukocyte antigen (HLA)-G
- The HLA-G gene is monomorphic and the only MHC antigen expressed on cytotrophoblast cells of placenta.
- Extravillous trophoblasts from normal human placenta and the BeWo adherent human choriocarcinoma cell line express an unusual form of class I HLA molecule.
- nonclassic HLA-G class I molecules in inhibiting natural killer cell function.
- modulate the immunologic relationship between mother and fetus.
- alternatively spliced HLA-G isoforms in first trimester trophoblast cell populations. Several alternatively spliced HLA-G mRNA isoforms, including a 14-bp polymorphism in the 3-prime UTR end (exon 8) of the HLA-G gene, are expressed at a significantly lower level than the corresponding HLA-G mRNA isoforms with the 14-bp sequence deleted. Hviid et al. (2003) suggested that this finding may have functional implications in connection with reports of aberrant HLA-G expression and reproductive success.
- alternative splicing that generates seven HLA-G proteins.

UniProt P17693 338 AA
- Involved in the presentation of foreign antigens to the immune system.
- Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells.

Methods

Protein Preparation

Protein Gel

Western Blotting

References

Search

5 Recent Publications: HLAG

Cite this page: Hill, M.A. (2024, April 27) Embryology Z3377257. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/User:Z3377257

What Links Here?

@@ Line 6: / Line 6: @@
 :'''Links:''' [[Abnormal_Development_-_Ectopic_Implantation|Ectopic Implantation]] | [[Trophoblast]] | [[Trophoblast - Protein Expression]] | [[Implantation]] | [[Week 2]] | [[Week 3]] | [[Placenta  Development]]
+==HLA-G==
+* [http://omim.org/entry/142871 OMIM 142871 - HLAG] HLA-6.0; HLA60, T-CELL A LOCUS; TCA
+** Human leukocyte antigen (HLA)-G
+** The HLA-G gene is monomorphic and the only MHC antigen expressed on cytotrophoblast cells of placenta.
+** Extravillous trophoblasts from normal human placenta and the BeWo adherent human choriocarcinoma cell line express an unusual form of class I HLA molecule.
+** nonclassic HLA-G class I molecules in inhibiting natural killer cell function.
+** modulate the immunologic relationship between mother and fetus.
+** alternatively spliced HLA-G isoforms in first trimester trophoblast cell populations. Several alternatively spliced HLA-G mRNA isoforms, including a 14-bp polymorphism in the 3-prime UTR end (exon 8) of the HLA-G gene, are expressed at a significantly lower level than the corresponding HLA-G mRNA isoforms with the 14-bp sequence deleted. Hviid et al. (2003) suggested that this finding may have functional implications in connection with reports of aberrant HLA-G expression and reproductive success.
+** alternative splicing that generates seven HLA-G proteins.
+*  [http://www.uniprot.org/uniprot/P17693  UniProt P17693] 338 AA
+** Involved in the presentation of foreign antigens to the immune system.
+**Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells.
 ==Methods==
-===RNA Preparation===
+===Protein Preparation===
-* [https://www.lifetechnologies.com/au/en/home/brands/product-brand/trizol.html Trizol]
+*
-===RNA Gel===
+===Protein Gel===
-* [http://biotium.com/product/gelredtm-nucleic-acid-gel-stain-10000x-in-water Gel Red]
+*
+===Western Blotting===
 ==References==
@@ Line 19: / Line 39: @@
 ===Search===
-Recent Publications: [http://www.ncbi.nlm.nih.gov/pubmed/?term=Ectopic+Pregnancy ''Ectopic Pregnancy'']
+Recent Publications: [http://www.ncbi.nlm.nih.gov/pubmed/?term=HLAG ''HLAG'']
-<pubmed limit=5>Ectopic Pregnancy</pubmed>
+<pubmed limit=5>HLAG</pubmed>
 {{Footer}}