Thymus Development

Introduction

Adult thymus location

The thymus has a key role in the development of an effective immune system as well as an endocrine function.

The mature thymus epithelium has two main cell types: cortical thymic epithelial (cTECs) and medullary thymic epithelial cells (mTECs) or stromal cells. These thymic stromal cells provide signals for T cell differentiation.

Links: original Endocrine Development - Thymus page

Some Recent Findings

Rodewald HR. Thymus organogenesis. Annu Rev Immunol. 2008;26:355-88. Boehm T, Bleul CC. The evolutionary history of lymphoid organs. Nat Immunol. 2007 Feb;8(2):131-5. "During vertebrate evolution, primary lymphoid organs appeared earlier than secondary lymphoid organs. Among the sites of primary lymphopoiesis during evolution and ontogeny, those for B cell differentiation have differed considerably, although they often have had myelolymphatic characteristics. In contrast, only a single site for T cell differentiation has occurred, exclusively the thymus." Assarsson E, Chambers BJ, Hogstrand K, Berntman E, Lundmark C, Fedorova L, Imreh S, Grandien A, Cardell S, Rozell B, Ljunggren HG. Severe defect in thymic development in an insertional mutant mouse model. J Immunol. 2007 Apr 15;178(8):5018-27.

Development Overview

The thymus and parathyroid are derived from 3rd pharyngeal pouches.

Development is a series of epithelial/mesenchymal inductive interactions between neural crest-derived arch mesenchyme and pouch endoderm. There is also the possibility that the surface ectoderm of 3rd pharyngeal clefts participates in thymus development.

Hassall's bodies form between 6 and 10 lunar months in humans. They appear after lymphopoiesis has been established and the cortex, medulla and the cortico-medullary junction are able to select of T lymphocytes undergoing progressive maturation. (Text modified from Bodey and Kaiser, 1997)

Experimental studies have shown that a neural crest contribution is also required during early thymic organogenesis.

MBoC Figure 24-6. The development and activation of T and B cells

Figure 24-7. Electron micrographs of nonactivated and activated lymphocytes

Development Changes

Fetal thymus anatomy

Fetal thymus

Changes with age Overall Size

• birth 10-15 g
• puberty 30-40 g
• after puberty - involution
  • Replaced by adipose tissue
  • middle-aged 10 g

Thymus Anatomy

• Superior mediastinum, anterior to heart
• Bilobed lymphoepithelial organ
  • Contains reticular cells but no fibers
• Stem lymphocytes
  • proliferate and differentiate
  • forms long-lived T- lymphocytes

Thymus Cells

• Reticular cells
  • Abundant, eosinophilic, large, ovoid and light nucleus 1-2 nucleoli
  • sheathe cortical capillaries
  • form an epitheloid layer
  • maintain microenvironment for development of T-lymphocytes in cortex (thymic epitheliocytes)
• Macrophages
  • cortex and medulla
  • difficult to distinguish from reticular cells in H&E
• Lymphocytes
  • cortex and medulla - more numerous (denser) in cortex
  • majority of them developing T-lymphocytes (= thymic lymphocytes or thymocytes)

Fetal/Young Thymus

Young medulla	Young cortex

Thymic corpuscle

Hassall’s corpuscle - Mass of concentric epithelioreticular cells

Adult Thymus

• Cortical lymphoid tissue is replaced by adipose tissue
• Increase in size of thymic corpuscles

Links: Blue Histology - Thymus

Hassall's Bodies

Fetal thymus showing Hassall's body

Hassall's bodies, also called Hassall's corpuscles, form between 6 and 10 lunar months in humans. They appear after lymphopoiesis has been established and the cortex, medulla and the cortico-medullary junction are able to select of T lymphocytes undergoing progressive maturation.Within the thymus their number increases until puberty, then decreases.

Named after Arthur Hill Hassall (1817-1894) a British physician and chemist.

Molecular Development

Cited2

Cited2 deletion in the mouse is embryonic lethal with cardiovascular malformations, adrenal agenesis, cranial ganglia fusion, exencephaly, and left-right patterning defects.

"Examination of Lmo4-deficient embryos revealed partially penetrant cardiovascular malformations and hypoplastic thymus. Examination of Lmo4;Cited2 compound mutants indicated that there is a genetic interaction between Cited2 and Lmo4 in control of thymus development. Our data suggest that this may occur, in part, through control of expression of a common target gene, Tbx1, which is necessary for normal thymus development."

Eva and Six

Both Eva and Six have been implicated in thymus development.

• Eya - human homolog of the Drosophila 'eyes absent' (Eya) gene.
• Six - vertebrate genes which are homologs of the Drosophila 'sine oculis' (so) gene.

References

Reviews

<pubmed>18403191</pubmed> <pubmed>16448532</pubmed> <pubmed>12969307</pubmed> <pubmed>11292256</pubmed>

Articles

<pubmed>11857615</pubmed>

Search PubMed: Thymus Development

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Cite this page: Hill, M.A. (2024, May 3) Embryology Thymus Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Thymus_Development

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G