Talk:In Vitro Oogenesis
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Cite this page: Hill, M.A. (2024, May 8) Embryology In Vitro Oogenesis. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:In_Vitro_Oogenesis |
2018
"Positive Regulation of RNA Metabolic Process" Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach
Biomed Res Int. 2018 Jan 10;2018:2863068. doi: 10.1155/2018/2863068. eCollection 2018.
Celichowski P1, Nawrocki MJ2, Dyszkiewicz-Konwińska M2,3, Jankowski M2, Budna J1, Bryja A2, Kranc W2, Borys S2, Knap S1,2, Ciesiółka S1, Jeseta M4, Piasecka-Stryczyńska K1, Khozmi R2, Bukowska D5, Antosik P5, Brüssow KP5, Bruska M2, Nowicki M1, Zabel M1,6, Kempisty B1,2,4.
Abstract
The cumulus-oocyte complexes (COCs) growth and development during folliculogenesis and oogenesis are accompanied by changes involving synthesis and accumulation of large amount of RNA and proteins. In this study, the transcriptomic profile of genes involved in "oocytes RNA synthesis" in relation to in vitro maturation in pigs was investigated for the first time. The RNA was isolated from oocytes before and after in vitro maturation (IVM). Interactions between differentially expressed genes/proteins belonging to "positive regulation of RNA metabolic process" ontology group were investigated by STRING10 software. Using microarray assays, we found expression of 12258 porcine transcripts. Genes with fold change higher than |2| and with corrected p value lower than 0.05 were considered as differentially expressed. The ontology group "positive regulation of RNA metabolic process" involved differential expression of AR, INHBA, WWTR1, FOS, MEF2C, VEGFA, IKZF2, IHH, RORA, MAP3K1, NFAT5, SMARCA1, EGR1, EGR2, MITF, SMAD4, APP, and NR5A1 transcripts. Since all of the presented genes were downregulated after IVM, we suggested that they might be significantly involved in regulation of RNA synthesis before reaching oocyte MII stage. Higher expression of "RNA metabolic process" related genes before IVM indicated that they might be recognized as important markers and specific "transcriptomic fingerprint" of RNA template accumulation and storage for further porcine embryos growth and development. PMID: 29546053 PMCID: PMC5818922 DOI: 10.1155/2018/2863068