Talk:In Vitro Oogenesis

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Cite this page: Hill, M.A. (2021, May 12) Embryology In Vitro Oogenesis. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:In_Vitro_Oogenesis

2018

Characterization of female germline stem cells from adult mouse ovaries and the role of rapamycin on them

Cytotechnology. 2018 Apr;70(2):843-854. doi: 10.1007/s10616-018-0196-6. Epub 2018 Jan 25.


Yang H1, Yao X1, Tang F1, Wei Y1, Hua J1, Peng S2.

Abstract

Germline stem cells (GSCs) play an indispensable role in establishing the fertility of an organism. The isolation and culture of adult female GSCs (FGSCs) have provided a robust foundation to study the development of female germ cells in rodents. However, many problems still need to be identified, such as the origin and location of FGSCs and the specific markers for screening. In this study, we acquired FGSCs that stably expressed Oct4 from Oct4 promoter-GFP transgenic mouse ovarian surface epithelium and cortical layer, and identified the cells possessing the representative features including the expression of GSCs marker genes and the potentiality of differentiation into all three germ layers in vitro. Moreover, rapamycin was confirmed to promote proliferation of mouse FGSCs and inhibit the differentiation capability in vivo. In addition to the reported disinfection function, rapamycin inhibited the activation of primordial follicles, as the inhibitor of mechanistic target of rapamycin pathway. These results will contribute to the study on folliculogenesis or oogenesis mechanism and have important implications on developing new technology and therapeutic approach in medicine for premature ovarian failure, infertility and even ovary remodelling in future. KEYWORDS: Characterization; Female germline stem cells; Mouse; Rapamycin PMID: 29372468 DOI: 10.1007/s10616-018-0196-6


"Positive Regulation of RNA Metabolic Process" Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach

Biomed Res Int. 2018 Jan 10;2018:2863068. doi: 10.1155/2018/2863068. eCollection 2018.

Celichowski P1, Nawrocki MJ2, Dyszkiewicz-Konwińska M2,3, Jankowski M2, Budna J1, Bryja A2, Kranc W2, Borys S2, Knap S1,2, Ciesiółka S1, Jeseta M4, Piasecka-Stryczyńska K1, Khozmi R2, Bukowska D5, Antosik P5, Brüssow KP5, Bruska M2, Nowicki M1, Zabel M1,6, Kempisty B1,2,4.

Abstract

The cumulus-oocyte complexes (COCs) growth and development during folliculogenesis and oogenesis are accompanied by changes involving synthesis and accumulation of large amount of RNA and proteins. In this study, the transcriptomic profile of genes involved in "oocytes RNA synthesis" in relation to in vitro maturation in pigs was investigated for the first time. The RNA was isolated from oocytes before and after in vitro maturation (IVM). Interactions between differentially expressed genes/proteins belonging to "positive regulation of RNA metabolic process" ontology group were investigated by STRING10 software. Using microarray assays, we found expression of 12258 porcine transcripts. Genes with fold change higher than |2| and with corrected p value lower than 0.05 were considered as differentially expressed. The ontology group "positive regulation of RNA metabolic process" involved differential expression of AR, INHBA, WWTR1, FOS, MEF2C, VEGFA, IKZF2, IHH, RORA, MAP3K1, NFAT5, SMARCA1, EGR1, EGR2, MITF, SMAD4, APP, and NR5A1 transcripts. Since all of the presented genes were downregulated after IVM, we suggested that they might be significantly involved in regulation of RNA synthesis before reaching oocyte MII stage. Higher expression of "RNA metabolic process" related genes before IVM indicated that they might be recognized as important markers and specific "transcriptomic fingerprint" of RNA template accumulation and storage for further porcine embryos growth and development. PMID: 29546053 PMCID: PMC5818922 DOI: 10.1155/2018/2863068