Talk:Hamster Development
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Cite this page: Hill, M.A. (2024, May 4) Embryology Hamster Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Hamster_Development |
2011
Hyperthermia induces upregulation of Connexin43 in the golden hamster neural tube
Birth Defects Res A Clin Mol Teratol. 2011 Sep 22. doi: 10.1002/bdra.22852. [Epub ahead of print]
Zhang J, Chen FZ, Gao Q, Sun JH, Tian GP, Gao YM. Source Department of Human Anatomy, Shandong University School of Medicine, Jinan, Shandong, People's Republic of China.
Abstract
BACKGROUND: During early embryonic development, maternal exposure to hyperthermia induces neural tube defects (NTDs). Connexins are essential for the formation of gap junctions and Connexin43 (Cx43) is crucially involved in neural tube development. This study was designed to explore the potential role of Cx43 in NTDs induced by hyperthermia.
METHODS: Using PCR, the Cx43 cDNA was screened from the cDNA library of the neural tube from golden hamsters treated with hyperthermia. By Northern blot, the expression of Cx43 in heat-treated and control groups of the golden hamsters at day 8.5 after mating was detected. Finally, by in situ hybridization and RT-PCR, the expression of Cx43 was examined in the neural tube at different time points after heat treatment.
RESULTS: Cx43 was stably expressed in heat-treated and control groups of the golden hamsters, whereas the expression was evidently higher in the heat-treated group. Cx43 expression in the neural tube at different time points after heat treatment was significantly higher than in control groups (p < 0.01). Hyperthermia did not induce any mutations in Cx43 cDNA.
CONCLUSIONS: Our data provide the first evidence that hyperthermia induces upregulation of Cx43 in the golden hamster neural tube. NTDs caused by hyperthermia may be intimately related with the overexpression of Cx43. Birth Defects Research (Part A) 2011. © 2011 Wiley-Liss, Inc.
Copyright © 2011 Wiley-Liss, Inc.
PMID 21954174
Biosynthesis of hamster zona pellucida is restricted to the oocyte
Theriogenology. 2011 Feb;75(3):463-72. Epub 2010 Nov 12.
Izquierdo-Rico MJ, Gimeno L, Jiménez-Cervantes C, Ballesta J, Avilés M. Source Department of Cell Biology and Histology, Faculty of Medicine, University of Murcia, Murcia, Spain.
Abstract The zona pellucida (ZP) is an extracellular coat that surrounds the mammalian oocyte and the early embryo until implantation. This coat mediates several critical aspects of fertilization, including species-selective sperm recognition, the blocking of polyspermy and protection of the oocyte and the preimplantation embryo. Depending on the species, the ZP is composed of three to four different glycoproteins encoded by three or four genes. These genes have been cloned and sequenced for different species. However, controversy exists about the cell type specificity of the ZP glycoproteins, for which several models have been proposed. Different groups have reported that ZP is produced only by the oocytes, by the granulosa cells or by both cell types, depending on the species under study. We recently described the expression of four ZP proteins in the hamster ovary. By means of the complete set of the hamster ZP cDNAs, we undertook the study of the origin and expression pattern of the four ZP genes. In the present work, the expression of ZP1, ZP2, ZP3 and ZP4 is carefully analyzed by in situ hybridization (ISH) in hamster ovaries. Our data suggest that the four hamster ZP genes are expressed in a coordinate and oocyte-specific manner during folliculogenesis. Furthermore, this expression is maximal during the first stages of the oocyte development and declines in oocytes from later development stages, particularly within large antral follicles.
Copyright © 2011 Elsevier Inc. All rights reserved.
PMID: 21074836 http://www.ncbi.nlm.nih.gov/pubmed/21074836
2009
Rpl30 and Hmgb1 are required for neurulation in golden hamster
Int J Neurosci. 2009;119(8):1076-90.
Yu L, Guan YJ, Gao Y, Wang X. Source Department of Histology and Embryology, Weifang Medical College, Weifang, China. Abstract Neural tube defects (NTDs) are a group of severe congenital malformations resulting from the failure of neurulation. Genes influencing neurulation have been investigated for their contribution to NTDs. Ribosomal protein (Rp) is an abundant and belongs to a high conservative gene family, which has the complex task of coordinating protein biosynthesis in order to maintain cell homeostasis and survival. However, the mechanisms of Rp in the NTDs are unknown. Understanding the mechanisms will lead to new insights into NTDs. In this report, we constructed a cDNA library from neural tube of golden hamster and screened the cDNA library by a subsection screening method (SSS). Our results demonstrate a possible essential role of the RPL30 cDNA gene during neurulation and in the risk of NTDs. Our study also suggests that another gene, HMGB1, may be significantly associated with neurulation and the risk of NTDs.
PMID 19922340
Collection and cryopreservation of hamster oocytes and mouse embryos
J Vis Exp. 2009 Mar 27;(25). pii: 1120. doi: 10.3791/1120. Costa-Borges N, González S, Ibáñez E, Santaló J. Source Unitat Biologia Cellular (Facultat de Biociències), Universitat Autònoma de Barcelona.
Abstract Embryos and oocytes were first successfully cryopreserved more than 30 years ago, when Whittingham et al. and Wilmut separately described that mouse embryos could be frozen and stored at -196 degrees C and, a few years later, Parkening et al. reported the birth of live offspring resulting from in vitro fertilization (IVF) of cryopreserved oocytes. Since then, the use of cryopreservation techniques has rapidly spread to become an essential component in the practice of human and animal assisted reproduction and in the conservation of animal genetic resources. Currently, there are two main methods used to cryopreserve oocytes and embryos: slow freezing and vitrification. A wide variety of approaches have been used to try to improve both techniques and millions of animals and thousands of children have been born from cryopreserved embryos. However, important shortcomings associated to cryopreservation still have to be overcome, since ice-crystal formation, solution effects and osmotic shock seem to cause several cryoinjuries in post-thawed oocytes and embryos. Slow freezing with programmable freezers has the advantage of using low concentrations of cryoprotectants, which are usually associated with chemical toxicity and osmotic shock, but their ability to avoid ice-crystal formation at low concentrations is limited. Slow freezing also induces supercooling effects that must be avoided using manual or automatic seeding. In the vitrification process, high concentrations of cryoprotectants inhibit the formation of ice-crystals and lead to the formation of a glasslike vitrified state in which water is solidified, but not expanded. However, due to the toxicity of cyroprotectants at the concentrations used, oocytes/embryos can only be exposed to the cryoprotectant solution for a very short period of time and in a minimum volume solution, before submerging the samples directly in liquid nitrogen. In the last decade, vitrification has become more popular because it is a very quick method in which no expensive equipment (programmable freezer) is required. However, slow freezing continues to be the most widely used method for oocyte/embryo cryopreservation. In this video-article we show, step-by-step, how to collect and slowly freeze hamster oocytes with high post-thaw survival rates. The same procedure can also be applied to successfully freeze and thaw mouse embryos at different stages of preimplantation development.
PMID: 19329926 http://www.ncbi.nlm.nih.gov/pubmed/19329926
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2789760/
http://www.jove.com/Details.stp?ID=1120
2008
The hamster as a model for embryo implantation: insights into a multifaceted process
Semin Cell Dev Biol. 2008 Apr;19(2):194-203. Epub 2007 Dec 4.
Reese J, Wang H, Ding T, Paria BC. Source Division of Neonatology, Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232-0656, USA. Abstract Defects in preimplantation embryonic development, uterine receptivity, and implantation are the leading cause of infertility, pregnancy problems and birth defects. Significant progress has been made in our basic understanding of these processes using the mouse model, where implantation is ovarian estrogen-dependent in the presence of progesterone. However, an animal model where implantation is progesterone-dependent must also be studied to gain a full understanding of the embryo and uterine events that are required for implantation. In this regard, the hamster is a useful model and this review summarizes the information currently available regarding mechanisms involved in synchronous preimplantation embryo and uterine development for implantation in this species.
PMID: 18178492
