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Cite this page: Hill, M.A. (2019, September 19) Embryology Hamster Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Hamster_Development
10 Most Recent Papers
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<pubmed limit=5>Hamster Development</pubmed>
<pubmed limit=5>Hamster Embryology</pubmed>
Cryopreservation and In Vitro culture of Preimplantation Embryos in Djungarian Hamster (Phodopus sungorus)
Reprod Domest Anim. 2015 Aug;50(4):677-83. doi: 10.1111/rda.12564. Epub 2015 Jun 18.
Brusentsev EY1, Abramova TO1, Rozhkova IN1, Igonina TN1, Naprimerov VA1, Feoktistova NY2, Amstislavsky SY1.
Although embryo cryobanking was applied to Syrian golden and to Campbell's hamsters, no attempt has been made at freezing embryos in Djungarian hamsters. Four-cell stage embryos were flushed from the reproductive ducts of pregnant females before noon of the third-day post coitum and frozen in 0.25-ml straws according to standard procedures of slow cooling. A mixture of permeating (ethylene glycol) and non-permeating (sucrose) cryoprotectants was used. The thawing was performed by incubating at RT for 40 s followed by 40 s in a water bath at 30.0°C. Most (66.7%) of the non-frozen four-cell embryos developed up to the morula stage in rat one-cell embryo culture medium (R1ECM). The use of hamster embryo culture medium (HECM) yielded fewer morulas (18.2%) during the same 24-h period of culture. The rate of embryo's surviving the freezing-thawing procedures, as estimated by light microscopy, was 60.7-68.8%. After 24-h culturing in R1ECM, 64.7% of frozen-thawed four-cell embryos developed and all of them reached the morula stage. Supplementation of R1ECM with GM-CSF (2 ng/ml) improved the rate of Djungarian hamster frozen-thawed embryo development: 100% of the four-cell stage embryos developed, 50% of them achieved the morula stage, and 50% developed even further and reached the blastocyst stage within 24 h of culturing. This study reports the world's first successful transfer of frozen-thawed Djungarian hamster embryos yielding term pups. Taken together, the results of this study demonstrate the possibility of applying some key reproductive technologies, that is, embryo freezing/cryopreservation and in vitro culture, to Djungarian hamsters. PMID: 26095791 DOI: 10.1111/rda.12564
Post-natal growth of the gastrointestinal tract of the Siberian hamster: morphometric analysis
Anat Histol Embryol. 2014 Dec;43(6):453-67. doi: 10.1111/ahe.12096. Epub 2013 Nov 22.
Wołczuk K1, Kobak J.
Post-natal growth of the gastrointestinal tract of the Siberian hamster was studied in newborn and 3-, 7-, 14-, 21-, 42- and 90-day-old animals. Morphometric measurements and calculations were carried out: length and internal surface of gastrointestinal tract segments, size (height, width, surface) and density of villi as well as allometric growth rate of the length and internal surface of the segments with respect to the body mass. The fastest growth rate of the gastrointestinal tract segments was noticed during the first 3 days of the post-natal life. Nevertheless, significant regional differences in their growth rate were found. The increase in the length and internal surface of the large intestine was fastest, while the smallest increase was observed in the oesophagus. All segments of the gastrointestinal tract except oesophagus exhibited a positive allometric relationship to the body mass from birth till final weaning, whereas during the post-weaning period, the increase was isometric. Thus, at birth, the gastrointestinal tract segments were relatively smaller compared with those observed in adults, but then, the gastrointestinal tract grew faster than the rest of the body and reached its adult proportions just before the transition to solid food. Most probably, reaching the adult structure of the gastrointestinal tract before the final weaning is an essential condition for the proper growth of an organism after the weaning. PMID: 24261618 DOI: 10.1111/ahe.12096
Perfluorooctanoic acid (PFOA) acts as a tumor promoter on Syrian hamster embryo (SHE) cells
Environ Sci Pollut Res Int. 2011 Aug;19(7):2537-49. doi: 10.1007/s11356-012-0968-z. Epub 2012 Aug 31.
Jacquet N, Maire MA, Rast C, Bonnard M, Vasseur P. Source Laboratory Interactions Ecotoxicology Biodiversity Ecosystems, University Paul Verlaine, CNRS UMR 7146, Rue du General Delestraint, 57070, Metz, France. email@example.com
Perfluorooctane sulfonate (PFOS) (C(8)F(17)SO(3)) and perfluorooctanoic acid (PFOA) (C(8)HF(15)O(2)) are synthetic chemicals widely used in industrial applications for their hydrophobic and oleophobic properties. They are persistent, bioaccumulative, and toxic to mammalian species. Their widespread distribution on earth and contamination of human serum raised concerns about long-term side effects. They are suspected to be carcinogenic through a nongenotoxic mode of action, a mechanism supported by recent findings that PFOS induced cell transformation but no genotoxicity in Syrian hamster embryo (SHE) cells. In the present study, we evaluated carcinogenic potential of PFOA using the cell transformation assay on SHE cells. The chemical was applied alone or in combination with a nontransformant concentration of benzo[a]pyrene (BaP, 0.4 μM) in order to detect PFOA ability to act as tumor initiator or tumor promoter. The results showed that PFOA tested alone in the range 3.7 × 10(-5) to 300 μM did not induce SHE cell transformation frequency in a 7-day treatment. On the other side, the combination BaP/PFOA induced cell transformation at all PFOA concentrations tested, which revealed synergistic effects. No genotoxicity of PFOA on SHE cells was detected using the comet assay after 5 and 24 h of exposure. No significant increase in DNA breakage was found in BaP-initiated cells exposed to PFOA in a 7-day treatment. The whole results showed that PFOA acts as a tumor promoter and a nongenotoxic carcinogen. Cell transformation in initiated cells was observed at concentrations equivalent to the ones found in human serum of nonoccupationally and occupationally exposed populations. An involvement of PFOA in increased incidence of cancer recorded in occupationally exposed population cannot be ruled out.
Hyperthermia induces upregulation of Connexin43 in the golden hamster neural tube
Birth Defects Res A Clin Mol Teratol. 2011 Sep 22. doi: 10.1002/bdra.22852. [Epub ahead of print]
Zhang J, Chen FZ, Gao Q, Sun JH, Tian GP, Gao YM. Source Department of Human Anatomy, Shandong University School of Medicine, Jinan, Shandong, People's Republic of China.
BACKGROUND: During early embryonic development, maternal exposure to hyperthermia induces neural tube defects (NTDs). Connexins are essential for the formation of gap junctions and Connexin43 (Cx43) is crucially involved in neural tube development. This study was designed to explore the potential role of Cx43 in NTDs induced by hyperthermia.
METHODS: Using PCR, the Cx43 cDNA was screened from the cDNA library of the neural tube from golden hamsters treated with hyperthermia. By Northern blot, the expression of Cx43 in heat-treated and control groups of the golden hamsters at day 8.5 after mating was detected. Finally, by in situ hybridization and RT-PCR, the expression of Cx43 was examined in the neural tube at different time points after heat treatment.
RESULTS: Cx43 was stably expressed in heat-treated and control groups of the golden hamsters, whereas the expression was evidently higher in the heat-treated group. Cx43 expression in the neural tube at different time points after heat treatment was significantly higher than in control groups (p < 0.01). Hyperthermia did not induce any mutations in Cx43 cDNA.
CONCLUSIONS: Our data provide the first evidence that hyperthermia induces upregulation of Cx43 in the golden hamster neural tube. NTDs caused by hyperthermia may be intimately related with the overexpression of Cx43. Birth Defects Research (Part A) 2011. © 2011 Wiley-Liss, Inc.
Copyright © 2011 Wiley-Liss, Inc.
Biosynthesis of hamster zona pellucida is restricted to the oocyte
Theriogenology. 2011 Feb;75(3):463-72. Epub 2010 Nov 12.
Izquierdo-Rico MJ, Gimeno L, Jiménez-Cervantes C, Ballesta J, Avilés M. Source Department of Cell Biology and Histology, Faculty of Medicine, University of Murcia, Murcia, Spain.
Abstract The zona pellucida (ZP) is an extracellular coat that surrounds the mammalian oocyte and the early embryo until implantation. This coat mediates several critical aspects of fertilization, including species-selective sperm recognition, the blocking of polyspermy and protection of the oocyte and the preimplantation embryo. Depending on the species, the ZP is composed of three to four different glycoproteins encoded by three or four genes. These genes have been cloned and sequenced for different species. However, controversy exists about the cell type specificity of the ZP glycoproteins, for which several models have been proposed. Different groups have reported that ZP is produced only by the oocytes, by the granulosa cells or by both cell types, depending on the species under study. We recently described the expression of four ZP proteins in the hamster ovary. By means of the complete set of the hamster ZP cDNAs, we undertook the study of the origin and expression pattern of the four ZP genes. In the present work, the expression of ZP1, ZP2, ZP3 and ZP4 is carefully analyzed by in situ hybridization (ISH) in hamster ovaries. Our data suggest that the four hamster ZP genes are expressed in a coordinate and oocyte-specific manner during folliculogenesis. Furthermore, this expression is maximal during the first stages of the oocyte development and declines in oocytes from later development stages, particularly within large antral follicles.
Copyright © 2011 Elsevier Inc. All rights reserved.
PMID: 21074836 http://www.ncbi.nlm.nih.gov/pubmed/21074836
Rpl30 and Hmgb1 are required for neurulation in golden hamster
Int J Neurosci. 2009;119(8):1076-90.
Yu L, Guan YJ, Gao Y, Wang X. Source Department of Histology and Embryology, Weifang Medical College, Weifang, China. Abstract Neural tube defects (NTDs) are a group of severe congenital malformations resulting from the failure of neurulation. Genes influencing neurulation have been investigated for their contribution to NTDs. Ribosomal protein (Rp) is an abundant and belongs to a high conservative gene family, which has the complex task of coordinating protein biosynthesis in order to maintain cell homeostasis and survival. However, the mechanisms of Rp in the NTDs are unknown. Understanding the mechanisms will lead to new insights into NTDs. In this report, we constructed a cDNA library from neural tube of golden hamster and screened the cDNA library by a subsection screening method (SSS). Our results demonstrate a possible essential role of the RPL30 cDNA gene during neurulation and in the risk of NTDs. Our study also suggests that another gene, HMGB1, may be significantly associated with neurulation and the risk of NTDs.
Collection and cryopreservation of hamster oocytes and mouse embryos
J Vis Exp. 2009 Mar 27;(25). pii: 1120. doi: 10.3791/1120. Costa-Borges N, González S, Ibáñez E, Santaló J. Source Unitat Biologia Cellular (Facultat de Biociències), Universitat Autònoma de Barcelona.
Abstract Embryos and oocytes were first successfully cryopreserved more than 30 years ago, when Whittingham et al. and Wilmut separately described that mouse embryos could be frozen and stored at -196 degrees C and, a few years later, Parkening et al. reported the birth of live offspring resulting from in vitro fertilization (IVF) of cryopreserved oocytes. Since then, the use of cryopreservation techniques has rapidly spread to become an essential component in the practice of human and animal assisted reproduction and in the conservation of animal genetic resources. Currently, there are two main methods used to cryopreserve oocytes and embryos: slow freezing and vitrification. A wide variety of approaches have been used to try to improve both techniques and millions of animals and thousands of children have been born from cryopreserved embryos. However, important shortcomings associated to cryopreservation still have to be overcome, since ice-crystal formation, solution effects and osmotic shock seem to cause several cryoinjuries in post-thawed oocytes and embryos. Slow freezing with programmable freezers has the advantage of using low concentrations of cryoprotectants, which are usually associated with chemical toxicity and osmotic shock, but their ability to avoid ice-crystal formation at low concentrations is limited. Slow freezing also induces supercooling effects that must be avoided using manual or automatic seeding. In the vitrification process, high concentrations of cryoprotectants inhibit the formation of ice-crystals and lead to the formation of a glasslike vitrified state in which water is solidified, but not expanded. However, due to the toxicity of cyroprotectants at the concentrations used, oocytes/embryos can only be exposed to the cryoprotectant solution for a very short period of time and in a minimum volume solution, before submerging the samples directly in liquid nitrogen. In the last decade, vitrification has become more popular because it is a very quick method in which no expensive equipment (programmable freezer) is required. However, slow freezing continues to be the most widely used method for oocyte/embryo cryopreservation. In this video-article we show, step-by-step, how to collect and slowly freeze hamster oocytes with high post-thaw survival rates. The same procedure can also be applied to successfully freeze and thaw mouse embryos at different stages of preimplantation development.
PMID: 19329926 http://www.ncbi.nlm.nih.gov/pubmed/19329926
The hamster as a model for embryo implantation: insights into a multifaceted process
Semin Cell Dev Biol. 2008 Apr;19(2):194-203. Epub 2007 Dec 4.
Reese J, Wang H, Ding T, Paria BC. Source Division of Neonatology, Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232-0656, USA. Abstract Defects in preimplantation embryonic development, uterine receptivity, and implantation are the leading cause of infertility, pregnancy problems and birth defects. Significant progress has been made in our basic understanding of these processes using the mouse model, where implantation is ovarian estrogen-dependent in the presence of progesterone. However, an animal model where implantation is progesterone-dependent must also be studied to gain a full understanding of the embryo and uterine events that are required for implantation. In this regard, the hamster is a useful model and this review summarizes the information currently available regarding mechanisms involved in synchronous preimplantation embryo and uterine development for implantation in this species.
Prostaglandins Leukot Med. 1982 Apr;8(4):399-402. Pregnancy and fetal development in hamsters treated with prostaglandin F2 alpha. Hilbelink DR, Chen LT, Lanning JC, Persaud TV. Abstract The influence of PGF2 alpha on pregnancy and fetal outcome was investigated in the hamster. Following subcutaneous treatment with 50, 100, 200, 400, and 800 micrograms PGF2 alpha prenatal loss was significantly increased only at the highest dose level. The offspring of the treated animals were all alive and normal. Fetal weight was not affected. However, following intravenous injection of 100 and 200 micrograms PGF2 alpha there was a significant reduction in fetal weight, and at the 400 micrograms dose level an increase in fetal indicate that PGF2 alpha is not teratogenic in hamsters despite the apparent greater sensitivity of the hamster embryo to prostaglandin. PMID: 6955808