Mouse Lymph Node Movie 8

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<mediaplayer width='477' height='360' image="http://embryology.med.unsw.edu.au/embryology/images/f/fd/Mouse_adult_lymph_node_08.jpg">File:Mouse_adult_lymph_node_08.mp4</mediaplayer> Mouse adult lymph node 08.jpg

Migration of the B cell-T cell conjugate in the stromal cell network in the lymph node.


Within lymph nodes T-helper cells activate B-cells, that then move to germinal centres and proliferate.


The movie shows the 45-min time lapse images recorded every 30 s.

  • green - cognate antigen-specific B cells
  • red - helper T cells are stably conjugated.
  • cyan - are primarily irradiation-resistant stromal cells.

The mouse lymph node was imaged ex vivo by TPLM 36 h after immunization. Elapsed time is shown as hours/minutes/seconds. The image volume is 100 μm (x)×100 μm (y)×60 μm (z). The scale bar shows 30 μm on the nearest x-y plane from the viewpoint.


Reference

<pubmed>20603709</pubmed>| PMC2937149

Copyright

Takaharu Okada, 2010

http://www.springer.com/open+access?SGWID=0-169302-12-833204-0

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Cite this page: Hill, M.A. (2019, September 17) Embryology Mouse Lymph Node Movie 8. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Lymph_Node_Movie_8

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© Dr Mark Hill 2019, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G