Mouse Lymph Node Movie 8

Embryology - 10 Dec 2023 Expand to Translate
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<html5media height="300" width="300">File:Mouse_adult_lymph_node_08.mp4</html5media>

Migration of the B cell-T cell conjugate in the stromal cell network in the lymph node.

Within lymph nodes T-helper cells(red) activate B-cells.
B-cells (green) then move to germinal centres and proliferate.
Mature B-cells stop dividing, migrate to regions around germinal centre (mantle zone).
Contact follicular dendritc cells (cyan, not bone marrow derived).

The movie shows the 45-min time lapse images recorded every 30 s.

green - cognate antigen-specific B cells
red - helper T cells are stably conjugated.
cyan - are primarily irradiation-resistant stromal cells.

The mouse lymph node was imaged ex vivo by TPLM 36 h after immunization. Elapsed time is shown as hours/minutes/seconds. The image volume is 100 μm (x)×100 μm (y)×60 μm (z). The scale bar shows 30 μm on the nearest x-y plane from the viewpoint.

Reference

<pubmed>20603709</pubmed>| PMC2937149

Copyright

Takaharu Okada, 2010

http://www.springer.com/open+access?SGWID=0-169302-12-833204-0

Springer Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. SpringerOpen creative commons 2 | Open Choice creative commons 4 | Open Access Factsheet (pdf, 96 kB)

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Cite this page: Hill, M.A. (2023, December 10) Embryology Mouse Lymph Node Movie 8. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Lymph_Node_Movie_8

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