Implantation microRNA: Difference between revisions
mNo edit summary |
|||
Line 3: | Line 3: | ||
[[File:Mark_Hill.jpg|thumb|150px|Dr Mark Hill]] | [[File:Mark_Hill.jpg|thumb|150px|Dr Mark Hill]] | ||
Ectopic pregnancy is a high-risk maternal medical condition with an approximate incidence of 1.5 to 2 % in reported pregnancies. In the USA, data from commercial health plans identified 11,989 ectopic pregnancies during the period from 2002 to 2007.<ref name=PMID20177279><pubmed>20177279</pubmed></ref> In the Australian state of New South Wales, in 2008 the annual hospitalisation rate for women with an ectopic pregnancy was 12.6 per 1000 births.<ref name=PMID22877595><pubmed>22877595</pubmed></ref> | Ectopic pregnancy is a high-risk maternal medical condition with an approximate incidence of 1.5 to 2 % in reported pregnancies. In the USA, data from commercial health plans identified 11,989 ectopic pregnancies during the period from 2002 to 2007.<ref name=PMID20177279><pubmed>20177279</pubmed></ref> In the Australian state of New South Wales, in 2008 the annual hospitalisation rate for women with an ectopic pregnancy was 12.6 per 1000 births.<ref name=PMID22877595><pubmed>22877595</pubmed></ref> | ||
microRNAs - 22 nucleotide noncoding ribonucleic acid (RNA) molecules. | |||
Revision as of 15:13, 2 March 2016
Embryology - 26 Apr 2024 Expand to Translate |
---|
Google Translate - select your language from the list shown below (this will open a new external page) |
العربية | català | 中文 | 中國傳統的 | français | Deutsche | עִברִית | हिंदी | bahasa Indonesia | italiano | 日本語 | 한국어 | မြန်မာ | Pilipino | Polskie | português | ਪੰਜਾਬੀ ਦੇ | Română | русский | Español | Swahili | Svensk | ไทย | Türkçe | اردو | ייִדיש | Tiếng Việt These external translations are automated and may not be accurate. (More? About Translations) |
Introduction
Ectopic pregnancy is a high-risk maternal medical condition with an approximate incidence of 1.5 to 2 % in reported pregnancies. In the USA, data from commercial health plans identified 11,989 ectopic pregnancies during the period from 2002 to 2007.[1] In the Australian state of New South Wales, in 2008 the annual hospitalisation rate for women with an ectopic pregnancy was 12.6 per 1000 births.[2]
microRNAs - 22 nucleotide noncoding ribonucleic acid (RNA) molecules.
This is new collaborative research project between clinical researchers from the Women and Childrens Hospital (RHW), basic researchers from the School of Medical Sciences (SoMS) and research support from the HSA Biobank and Bioanalytical Mass Spectrometry Facility ( BMSF).
Our research group has established a uterine tube Biobank and cell culture techniques in order to investigate factors and markers associated with tubal ectopic pregnancies.
This page introduces the project and provides some introductory information for students who may wish to carry out an Honours, Independent Research Project or higher degree in the research laboratory. Student Projects | 2014 Biomed Expo
- Research Links: 2014 Seminar | Dr Mark Hill | Professor William Ledger | Royal Hospital for Women | SOMS | HSA Biobank | BMSF
- Ectopic Page Links: Ectopic Implantation | Implantation | Week 2 | Placenta - Abnormalities | Trophoblast | Trophoblast - Protein Expression | Implantation microRNA
Some Recent Findings
More recent papers |
---|
This table allows an automated computer search of the external PubMed database using the listed "Search term" text link.
More? References | Discussion Page | Journal Searches | 2019 References | 2020 References Search term: Ectopic Pregnancy <pubmed limit=5>Ectopic Pregnancy</pubmed> Search term: Implantation microRNA <pubmed limit=5>Implantation microRNA</pubmed> |
miRNA signature and Dicer requirement during human endometrial stromal decidualization in vitro
PLoS One. 2012;7(7):e41080. doi: 10.1371/journal.pone.0041080. Epub 2012 Jul 20.
Estella C1, Herrer I, Moreno-Moya JM, Quiñonero A, Martínez S, Pellicer A, Simón C.
Abstract
Decidualization is a morphological and biochemical transformation of endometrial stromal fibroblast into differentiated decidual cells, which is critical for embryo implantation and pregnancy establishment. The complex regulatory networks have been elucidated at both the transcriptome and the proteome levels, however very little is known about the post-transcriptional regulation of this process. miRNAs regulate multiple physiological pathways and their de-regulation is associated with human disorders including gynaecological conditions such as endometriosis and preeclampsia. In this study we profile the miRNAs expression throughout human endometrial stromal (hESCs) decidualization and analyze the requirement of the miRNA biogenesis enzyme Dicer during this process. A total of 26 miRNAs were upregulated and 17 miRNAs downregulated in decidualized hESCs compared to non-decidualized hESCs. Three miRNAs families, miR-181, miR-183 and miR-200, are down-regulated during the decidualization process. Using miRNAs target prediction algorithms we have identified the potential targets and pathways regulated by these miRNAs. The knockdown of Dicer has a minor effect on hESCs during in vitro decidualization. We have analyzed a battery of decidualization markers such as cell morphology, Prolactin, IGFBP-1, MPIF-1 and TIMP-3 secretion as well as HOXA10, COX2, SP1, C/EBPß and FOXO1 expression in decidualized hESCs with decreased Dicer function. We found decreased levels of HOXA10 and altered intracellular organization of actin filaments in Dicer knockdown decidualized hESCs compared to control. Our results provide the miRNA signature of hESC during the decidualization process in vitro. We also provide the first functional characterization of Dicer during human endometrial decidualization although surprisingly we found that Dicer plays a minor role regulating this process suggesting that alternative biogenesis miRNAs pathways must be involved in human endometrial decidualization.
PMID 22911744
- miR-181, miR-183 and miR-200 miRNAs Families Members are Similarly down-regulated during in vitro Decidualization
- For the mir-181 family, which includes six miRNAs precursors (mir-181a-1, mir-181a-2, mir-181b-1, mir-181b-2, mir-181c and mir-181d) , the corresponding mature miRNA expression in decidual cells decreased
- miR-96 and miR-135b Regulates FOXO and HOXA10 Expression and IGFBP-1 Secretion
- two miRNAs that decrease their expression during stromal differentiation
- other miRNA family includes miR-96, miR-182 and miR-183 with potential molecular pathways implicated in actin cytoskeleton reorganization, such as RAC1 and ITGß1, and both genes are implicated in endometrial decidualization
- total 33 differentially expressed miRNAs during our in vitro decidualization model, only two (miR-181b and miR-181d) have been previously identified in the Qian et al study
miRNA Target Prediction
To identify potential miRNA target genes, we used four publicly available target prediction algorithms: targetscan (http://www.targetscan.org/), miRanda (microRNA.org), microCosm (http://www.ebi.ac.uk/enright-srv/microcosm/htdocs/targets/v5/) and PicTar (http://pictar.mdc-berlin.de/). The Diana miR-Path database is a web-based computational tool developed to identify the molecular pathways potentially altered by the expression of single or multiple microRNAs [21] (http://diana.cslab.ece.ntua.gr/pathways/).
Differential expression of microRNAs with progression of gestation and inflammation in the human chorioamniotic membranes
Am J Obstet Gynecol. 2007 Sep;197(3):289.e1-6.
Montenegro D1, Romero R, Pineles BL, Tarca AL, Kim YM, Draghici S, Kusanovic JP, Kim JS, Erez O, Mazaki-Tovi S, Hassan S, Espinoza J, Kim CJ.
Abstract
OBJECTIVE: The aim of this study was to identify differential expression of microRNAs (miRNAs) in chorioamniotic membranes with advancing gestation, labor, and inflammation. STUDY DESIGN: Expression profiles of 157 miRNAs in the chorioamniotic membranes were obtained from patients in the following groups: 1) term not in labor (n = 10); 2) term in labor (n = 10); 3) preterm labor with histologic chorioamionitis (n = 9); and 4) without histologic chorioamnionitis (n = 10). RESULTS: More than 95% of the miRNAs screened were expressed. Gestational age-dependent changes in expression were observed for 13 miRNAs. No differences in miRNA expression were observed between women without labor and women in labor. Membranes with chorioamnionitis displayed increased expression of miR-223 and miR-338. Gene Ontology analysis of genes targeted by differentially expressed miRNAs revealed enrichment for specific biological process categories. CONCLUSION: Chorioamniotic membranes with advancing gestational age and chorioamnionitis are associated with the differential expression of a subset of miRNAs. PMID 17826424
Cite this page: Hill, M.A. (2024, April 26) Embryology Implantation microRNA. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Implantation_microRNA
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G