File:Zygote pronuclei stages 01.jpg: Difference between revisions

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Mouse Zygote Pronuclei Stages

Mouse zygotes at pronuclear stages, male pronucleus initially contains 5-methylcytosine (5mC, red) oxidises to form 5-hydroxymethylcytosine (5hmC, green).

PN1/PN2 (Early)
PN3 (Mid)
PN4/PN5 (Late)

5hmC - 5-hydroxymethylcytosine (formed by enzymatic oxidation of 5mC).
5mC - 5-methylcytosine.

Links: Epigenetics | Zygote | Mouse Development

Reference

<pubmed>21321204</pubmed>| PMC2132672 | PNAS

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Proceedings National Academy of Sciences (PNAS) Liberalization of PNAS copyright policy: Noncommercial use freely allowed Note original Author should be contacted for permission to reuse for Educational purposes. See also PNAS Author Rights and Permission FAQs

Cozzarelli NR, Fulton KR, Sullenberger DM. Liberalization of PNAS copyright policy: noncommercial use freely allowed. Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12399. PMID15314225 "Our guiding principle is that, while PNAS retains copyright, anyone can make noncommercial use of work in PNAS without asking our permission, provided that the original source is cited."

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048122/figure/fig02/

Figure 2 Panel A cropped and resized from original figure.

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	Date/Time	Thumbnail	Dimensions	User	Comment
current	09:04, 16 June 2012		1,194 × 460 (49 KB)	Z8600021 (talk \| contribs)	==Mouse Zygote Pronuclei Stages== Mouse zygotes male pronucleus initially contains 5-methylcytosine (5mC, red) oxidises to form 5-hydroxymethylcytosine (5hmC, green). 5hmC and 5mC in early pronuclear stage zygotes. (A) Zygotes at pronuclear stages PN1,

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 ==Mouse Zygote Pronuclei Stages==
-Mouse zygotes male pronucleus initially contains 5-methylcytosine (5mC, red) oxidises to form 5-hydroxymethylcytosine (5hmC, green).
+Mouse zygotes at pronuclear stages, male pronucleus initially contains 5-methylcytosine (5mC, red) oxidises to form 5-hydroxymethylcytosine (5hmC, green).
-hmC and 5mC in early pronuclear stage zygotes. (A) Zygotes at pronuclear stages PN1, PN2, and PN3 were double-stained with anti-5hmC antibody (green) and anti-5mC antibody (red). Merged images are shown. (B) The levels of 5hmC and 5mC in paternal and maternal pronuclei were quantitated. The ratio of staining signal between the paternal and maternal pronucleus is plotted. The number of zgotes analyzed in PN1/PN2 (Early), in PN3 (Mid), and in PN4/PN5 (Late) are indicated with n values. The median value is indicated by a horizontal line and a number. The difference between each two datasets is statistically significant, as seen in the P values of t-tests.
+* PN1/PN2 (Early)
+* PN3 (Mid)
+* PN4/PN5 (Late)
 * '''5hmC''' - 5-hydroxymethylcytosine (formed by enzymatic oxidation of 5mC).
@@ Line 18: / Line 20: @@
-http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048122/figure/fig01/
+http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048122/figure/fig02/
+Figure 2 Panel A cropped and resized from original figure.
 [[Category:Mouse]] [[Category:Zygote]] [[Category:Epigenetics]] [[Category:Fluorescence]]