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Epigenetic Profiles of SCNT Bovine Embryos

Epigenetic profiles of homotype SCNT (A-A and B-B), heterotype SCNT (A-B and B-A), and IVF embryos.

The bovine embryos were double stained for histone H3K9 methylation (green) and DNA (blue, stained with DAPI to identify the nuclear compartment).

The right column shows representative images of the anti-histone H3-dimethylK9 (H3K9) immunofluorescence from IVF embryos (a, n = 35), A-A SCNT embryos (b, n = 11), B-B SCNT embryos (c, n = 17), A-B SCNT embryos (d, n = 12), and B-A SCNT embryos (e, n = 13). Blastocysts from IVF (a) and homotype SCNT (A-A and B-B) treatments (b, c) showed hypomethylated trophectoderm and hypermethylated ICM, whereas heterotype SCNT (A-B and B-A) blastocysts (d, e) had a more homogeneous pattern between trophectoderm and ICM.


Links: epigenetics | bovine | blastocyst | stem cells

Reference

Burgstaller JP, Schinogl P, Dinnyes A, Müller M & Steinborn R. (2007). Mitochondrial DNA heteroplasmy in ovine fetuses and sheep cloned by somatic cell nuclear transfer. BMC Dev. Biol. , 7, 141. PMID: 18154666 DOI.

Copyright

© 2007 Burgstaller et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cite this page: Hill, M.A. (2024, April 24) Embryology Epigenetic profiles of SCNT bovine embryos.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Epigenetic_profiles_of_SCNT_bovine_embryos.jpg

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current14:17, 23 August 2012Thumbnail for version as of 14:17, 23 August 2012497 × 852 (72 KB)Z8600021 (talk | contribs)==Epigenetic Profiles of SCNT Bovine Embryos== Epigenetic profiles of homotype SCNT (A-A and B-B), heterotype SCNT (A-B and B-A), and IVF embryos. The bovine embryos were double stained for histone H3K9 methylation (green) and DNA (blue, stained with DAP

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