Movie - Early Division Chromosomes: Difference between revisions
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| < | | <wikiflv width="380" height="400" autostart="false" position="left">Mouse_zygote_division_02.flv|File:Mouse_zygote_division_02_icon.jpg</wikiflv> | ||
[[Media:Mouse_zygote_division_02.mov|Quicktime movie]] | [[Media:Mouse_zygote_division_02.mov|Quicktime movie]] | ||
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Movie shows mitotic division of the early mouse embryo starting at the zygote stage. | |||
The elapsed time from the start of acquisition (days:hours:minutes) is indicated. | The elapsed time from the start of acquisition (days:hours:minutes) is indicated. | ||
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Maximum projections of labeled histone protein (H2B-mRFP; top left) and phosphorylated H3 (H3S10ph, Fab313-488; top right) are shown with brightfield (BF; bottom left) and in merged images (bottom right; H2B and Fab313 are shown in cyan and red, respectively). | Maximum projections of labeled histone protein (H2B-mRFP; top left) and phosphorylated H3 (H3S10ph, Fab313-488; top right) are shown with brightfield (BF; bottom left) and in merged images (bottom right; H2B and Fab313 are shown in cyan and red, respectively). | ||
Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19) | |||
DNA in eukaryotes is wrapped around a histone octamer containing H2A, H2B, H3, and H4, forming a nucleosome, which is the fundamental unit of chromatin. | DNA in eukaryotes is wrapped around a histone octamer containing H2A, H2B, H3, and H4, forming a nucleosome, which is the fundamental unit of chromatin. | ||
'''Links:''' [[ | '''Links:''' [[Quicktime Movie - Early Division Chromosomes|Quicktime version]] | [[Movie - Early Division Chromosomes|Flash version]] | ||
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===Reference=== | |||
==Reference== | |||
<pubmed>19995936</pubmed>| [http://jcb.rupress.org/content/187/6/781.full JCB] | <pubmed>19995936</pubmed>| [http://jcb.rupress.org/content/187/6/781.full JCB] | ||
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{{ | {{JCB}} | ||
{{Glossary}} | |||
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[[Category:Mouse]] [[Category:Zygote]] [[Category:Mitosis]] | [[Category:Mouse]] [[Category:Zygote]] [[Category:Mitosis]] | ||
Latest revision as of 08:45, 7 March 2013
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| File:Mouse_zygote_division_02_icon.jpg</wikiflv> |
Movie shows mitotic division of the early mouse embryo starting at the zygote stage.
Maximum projections of labeled histone protein (H2B-mRFP; top left) and phosphorylated H3 (H3S10ph, Fab313-488; top right) are shown with brightfield (BF; bottom left) and in merged images (bottom right; H2B and Fab313 are shown in cyan and red, respectively). Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19) DNA in eukaryotes is wrapped around a histone octamer containing H2A, H2B, H3, and H4, forming a nucleosome, which is the fundamental unit of chromatin. Links: Quicktime version | Flash version |
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Mouse embryo was injected with H2B-mRFP mRNA and Fab313-488. Images of 51 focal planes (2-µm intervals) were captured at 10-min intervals using an inverted microscope (IX-71) with an UPlan-Apochromat 40× NA 1.0 oil immersion objective lens. Maximum projections of H2B-mRFP (top left) and Fab313-488 (top right) are shown with brightfield (BF; bottom left) and merged images (bottom right; H2B and Fab311 are shown in cyan and red, respectively).
The elapsed time from the start of acquisition (days:hours:minutes) is indicated. Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19), as it recognizes histone H3S10ph next to di- or trimethylated H3K9. |
Reference
<pubmed>19995936</pubmed>| JCB
Published December 14, 2009 // JCB vol. 187 no. 6 781-790 The Rockefeller University Press, doi: 10.1083/jcb.200904137 Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase
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Cite this page: Hill, M.A. (2024, June 27) Embryology Movie - Early Division Chromosomes. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Movie_-_Early_Division_Chromosomes
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
