Mouse Zygote 2 Movie

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Mouse zygote division 02 icon.jpg

Movie shows mitotic division of the early mouse embryo starting at the zygote stage.


The elapsed time from the start of acquisition (days:hours:minutes) is indicated.

Maximum projections of labeled histone protein (H2B-mRFP; top left) and phosphorylated H3 (H3S10ph, Fab313-488; top right) are shown with brightfield (BF; bottom left) and in merged images (bottom right; H2B and Fab313 are shown in cyan and red, respectively).


Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19)

DNA in eukaryotes is wrapped around a histone octamer containing H2A, H2B, H3, and H4, forming a nucleosome, which is the fundamental unit of chromatin.


Links: MP4 version | Mouse Zygote 1 Movie | Cell Division - Mitosis | Zygote | Morula | Week 1 | Movies
Mouse zygote division 02 icon.jpg Mouse embryo was injected with H2B-mRFP mRNA and Fab313-488. Images of 51 focal planes (2-µm intervals) were captured at 10-min intervals using an inverted microscope (IX-71) with an UPlan-Apochromat 40× NA 1.0 oil immersion objective lens.
  • top left- Maximum projections of H2B-mRFP
  • top right - Fab313-488
  • bottom left - brightfield (BF)
  • bottom right - merged images, H2B (cyan) and Fab311 (red).

The elapsed time from the start of acquisition (days:hours:minutes) is indicated.

Fab313-488 is preferentially concentrated on maternal condensed chromosomes at the first division (03:19–05:19), as it recognizes histone H3S10ph next to di- or trimethylated H3K9.

Reference

Yoko Hayashi-Takanaka, Kazuo Yamagata, Naohito Nozaki, Hiroshi Kimura Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase. J. Cell Biol.: 2009, 187(6);781-90 PubMed 19995936

| JCB

Published December 14, 2009 // JCB vol. 187 no. 6 781-790 The Rockefeller University Press, doi: 10.1083/jcb.200904137 Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase


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Cite this page: Hill, M.A. 2017 Embryology Mouse Zygote 2 Movie. Retrieved December 18, 2017, from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Zygote_2_Movie

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© Dr Mark Hill 2017, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G