Talk:Episcopic Fluorescence Image Capture
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Cite this page: Hill, M.A. (2024, May 24) Embryology Episcopic Fluorescence Image Capture. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Episcopic_Fluorescence_Image_Capture |
2015
Spatial Change of Cruciate Ligaments in Rat Embryo Knee Joint by Three-Dimensional Reconstruction
PLoS One. 2015 Jun 22;10(6):e0131092. doi: 10.1371/journal.pone.0131092. eCollection 2015.
Zhang X1, Aoyama T1, Takaishi R1, Higuchi S1, Yamada S2, Kuroki H1, Takakuwa T1.
Abstract
This study aimed to analyze the spatial developmental changes of rat cruciate ligaments by three-dimensional (3D) reconstruction using episcopic fluorescence image capture (EFIC). Cruciate ligaments of Wister rat embryos between embryonic day (E) 16 and E20 were analyzed. Samples were sectioned and visualized using EFIC. 3D reconstructions were generated using Amira software. The length of the cruciate ligaments, distances between attachment points to femur and tibia, angles of the cruciate ligaments and the cross angle of the cruciate ligaments were measured. The shape of cruciate ligaments was clearly visible at E17. The lengths of the anterior cruciate ligament (ACL) and posterior cruciate ligament (PCL) increased gradually from E17 to E19 and drastically at E20. Distances between attachment points to the femur and tibia gradually increased. The ACL angle and PCL angle gradually decreased. The cross angle of the cruciate ligaments changed in three planes. The primordium of the 3D structure of rat cruciate ligaments was constructed from the early stage, with the completion of the development of the structures occurring just before birth.
PMID 26098761 PMCID: PMC4476736 DOI: 10.1371/journal.pone.0131092
2010
Developmental atlas of the early first trimester human embryo
Dev Dyn. 2010 Jun;239(6):1585-95. doi: 10.1002/dvdy.22316.
Yamada S1, Samtani RR, Lee ES, Lockett E, Uwabe C, Shiota K, Anderson SA, Lo CW.
Abstract
Rapid advances in medical imaging are facilitating the clinical assessment of first-trimester human embryos at increasingly earlier stages. To obtain data on early human development, we used magnetic resonance (MR) imaging and episcopic fluorescence capture (EFIC) to acquire digital images of human embryos spanning the time of dynamic tissue remodeling and organogenesis (Carnegie stages 13 to 23). These imaging data sets are readily resectioned digitally in arbitrary planes, suitable for rapid high-resolution three-dimensional (3D) observation. Using these imaging datasets, a web-accessible digital Human Embryo Atlas (http://apps.devbio.pitt.edu/humanatlas/) was created containing serial 2D images of human embryos in three standard histological planes: sagittal, frontal, and transverse. In addition, annotations and 3D reconstructions were generated for visualizing different anatomical structures. Overall, this Human Embryo Atlas is a unique resource that provides morphologic data of human developmental anatomy that can accelerate basic research investigations into developmental mechanisms that underlie human congenital anomalies.
PMID 20503356 PMCID: PMC3401072 DOI: 10.1002/dvdy.22316
2002
Phenotyping transgenic embryos: a rapid 3-D screening method based on episcopic fluorescence image capturing
Nat Genet. 2002 Jan;30(1):59-65. Epub 2001 Dec 17.
Weninger WJ1, Mohun T.
Abstract
We describe a technique suitable for routine three-dimensional (3-D) analysis of mouse embryos that is based on episcopic fluorescence images captured during serial sectioning of wax-embedded specimens. We have used this procedure to describe the cardiac phenotype and associated blood vessels of trisomic 16 (Ts16) and Cited2-null mutant mice, as well as the expression pattern of an Myf5 enhancer/beta-galactosidase transgene. The consistency of the images and their precise alignment are ideally suited for 3-D analysis using video animations, virtual resectioning or commercial 3-D reconstruction software packages. Episcopic fluorescence image capturing (EFIC) provides a simple and powerful tool for analyzing embryo and organ morphology in normal and transgenic embryos.
PMID 11743576 DOI: 10.1038/ng785
