Talk:ANAT2341 Lab 2

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Effect of pre-in vitro maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle

J Reprod Dev. 2018 Jun 22;64(3):233-241. doi: 10.1262/jrd.2018-009. Epub 2018 Mar 2.

Sugimura S1, Yamanouchi T2, Palmerini MG3, Hashiyada Y2, Imai K4, Gilchrist RB5.


The administration of follicle-stimulating hormone (FSH) prior to oocyte retrieval improves oocyte developmental competence. During bovine embryo production in vitro, however, oocytes are typically derived from FSH-unprimed animals. In the current study, we examined the effect of pre-in vitro maturation (IVM) with cAMP modulators, also known as the second messengers of FSH, on the developmental competence of oocytes derived from small antral follicles (2-4 mm) of FSH-unprimed animals. Pre-IVM with N6,2'-O-dibutyryladenosine 3',5'-cyclicmonophosphate (dbcAMP) and 3-isobutyl-1-methylxanthine (IBMX) for 2 h improved the blastocyst formation in oocytes stimulated by FSH or amphiregulin (AREG). Furthermore, pre-IVM enhanced the expression of the FSH- or AREG-stimulated extracellular matrix-related genes HAS2, TNFAIP6, and PTGS2, and epidermal growth factor (EGF)-like peptide-related genes AREG and EREG. Additionally, pre-IVM with dbcAMP and IBMX enhanced the expression of EGFR, and also increased and prolonged cumulus cell-oocyte gap junctional communication. The improved oocyte development observed using the pre-IVM protocol was ablated by an EGF receptor phosphorylation inhibitor. These results indicate that pre-IVM with cAMP modulators could contribute to the acquisition of developmental competence by bovine oocytes from small antral follicles through the modulation of EGF receptor signaling and oocyte-cumulus/cumulus-cumulus gap junctional communication. KEYWORDS: Bovine; EGFR signaling; Embryo development; Gap junction; Pre-IVM PMID: 29503399 PMCID: PMC6021610 DOI: 10.1262/jrd.2018-009

Associate Professor Robert Gilchrist Dr Hayden Homer
Talk - The Reproductive Technology Revolution Talk - Meiosis in Mammalian Oocytes and Age-related Vulnerability
Dr Gilchrist’s primary research interests are in the regulation of mammalian oocyte development and maturation, and the development of novel oocyte maturation techniques for infertility treatment. Dr Homer leads a research programme aimed at understanding the molecular basis of oocyte quality and its striking decline with aging, especially as women go beyond their mid-thirties.
UNSW Research Gateway - PubMed UNSW Research Gateway - PubMed
Oocyte BMP15 and GDF9 effects.jpg

Oocyte BMP15 and GDF9 effects PMID 25058588

Meiosis sister kinetochore geometry.jpg

Meiosis sister kinetochore geometry PMID 24642833

How separated sisters get bad connections

Cell Cycle. 2014 Apr 15;13(8):1222-3. doi: 10.4161/cc.28567. Epub 2014 Mar 18.

Homer HA.

KEYWORDS: aneuploidy; cohesin; kinetochore; meiosis I; meiosis II; microtubule attachment; mouse oocyte

Comment on Kinetochore microtubule establishment is defective in oocytes from aged mice. [Cell Cycle. 2014]

PMID 24642833

2012 Lab 2

ANAT2341 Lab 2: Introduction | Fertilization | Week 1 | Week 2 | Online Assessment | Group Project