Talk:2018 Group Project 5
|Projects 2018: 1 Adrenal Medulla | 3 Melanocytes | 4 Cardiac | 5 Dorsal Root Ganglion|
- 1 About this Discussion Page
- 2 Project Assessment
- 3 Peer Reviews (Lab 10)
- 4 Introduction
- 5 History
- 6 Embryonic Origins
- 7 Developmental Process
- 8 Adult Function
- 9 Tissue / Organ structure
- 10 Molecular Mechanisms / Factors / Genes
- 11 Abnormalities / Abnormal development
- 12 Animal Models
- 13 Current research (Labs)
- 14 Glossary
- 15 Reference
About this Discussion Page
The project discussion page is where your group members can post discussion on the project topic. This will be demonstrated in the practical tutorial in week 3.
Please follow these 3 simple rules:
- Never identify yourself or any other students by name, use only your student number.
- Only edit your own student page or your own group project page.
- Only add content that is both correctly cited and you have permission to reuse.
|Group Assessment Criteria|
| Science Student Projects
|More Information on Assessment Criteria | Science Student Projects|
- History section covers early discoverers, but not a complete more recent timeline of DRG discoveries. For example, cell death and Nerve Growth Factor (NGF) during development. Hamburger V. (1992). History of the discovery of neuronal death in embryos. J. Neurobiol. , 23, 1116-23. PMID: 1469378 DOI.
- Molecular - useful linking molecular factors to OMIM database demonstrates extension of the project content beyond the project page.
- Timeline of Neurogenesis - section provides a good summary of the mouse model of neurogenesis. Note that while there has been a general matching of mouse days to human days of development, this does not necessarily describe the specific mouse events in the project page timeline, and should not have been included here.
- Videos - relevant to project topic.
- Glossary - appears to focus on anatomical directions and only a few listed terms (5). This section should have included the acronyms (for example - CRPS, FZD, SCS, Nrg) and terminology.
- Disorders - included some relevant disorders, such as Sjögren Syndrome and Sensory Ganglionitis. These are both "adult" disorders, should have included something more relevant to development abnormalities (congenital). Though for Sjögren Syndrome no molecular aspects such as aquaporins (AQPs) and anti AQPs autoantibodies in this section. Soyfoo MS, Chivasso C, Perret J & Delporte C. (2018). Involvement of Aquaporins in the Pathogenesis, Diagnosis and Treatment of Sjögren's Syndrome. Int J Mol Sci , 19, . PMID: 30380700 DOI.
- References - not clear why a (unnecessary) numbered list of references have been included at the start of the project page reference list? Total project 93 references. Recent project page references (5 years): 2018 (2), 2017 (3), 2016 (4), 2015 (4) and 2014 (2). Note PubMed results for "dorsal root ganglia development" the there are currently more than 4,500 articles, with last 5 years 764 articles. No specific identification (separation) of research articles and review papers within the project page?
- Z5229597 - 567
- Z5229438 - 208
- Z5229431 - 158
- Z5229399 - 46 total edits; only 3 significant additions; (0) no change edit = 5;
- Assessment feedback provided on figure page. 16 images.
- Six images sourced from Wikipedia rather than scientific research literature. This was described in class tutorials as not an acceptable source for your project work and only a minimal number of images (1-2) should have been sourced from here.
- Z5229438 - 6 images (3 Wikipedia)
- Z5229431 - 5 images (2 Wikipedia)
- Z5229597 - 4 images (1 Wikipedia)
- Z5229399 - 1 image
Z5229399 (talk) 11:33, 14 August 2018 (AEST) Z5229597 (talk) 11:34, 14 August 2018 (AEST) Z5229431 (talk) 11:35, 14 August 2018 (AEST) Z5229549 (talk) 11:36, 14 August 2018 (AEST) Z5229438 (talk) 11:36, 21 August 2018 (AEST)
Our group communicated over Facebook Messenger for the majority of the project. That is why there is not too much activity on our discussion page. Every member of our group was consistently active on our online group chat, and we were able to very quickly discuss any problems that came up from there.
Peer Reviews (Lab 10)
In embryonic origins, Dorsal root ganglion was mentioned as DRG in the 2nd paragraph. You might want to introduce this abbreviation beside the term at the first paragraph: Dorsal Root Ganglion (DRG) so that the reader can easily understand what you are referring to.
Some typos can be seen through out the wiki page like migratio and the format of the referencing is not consistent "lateral to the neural tube. ." and "lowed quickly by the precursors that shape the development of TrkA.." as compared to other parts of the wiki: "during later stages following migration. "
Content wise, the project seems to be doing fine with tons of references and content (with exception of the empty sections like History).
The videos were not uploaded on the page properly (under the current research section), so you might want to fix that.
Z5091101 (talk) 20:14, 3 October 2018 (AEST)z5091101Z5091101 (talk) 20:14, 3 October 2018 (AEST) There has been an extensive use of references which is great especially since this topic seems to be really complex. Maybe a few more images for the beginning part of the article will make it look more user-friendly. Definitely have a look over for any grammar/spelling issues.
- Embryonic origins has been well-written. Proof-read for typing errors. Neural crest migration section shows good research and use of terminology. Neuronal and glial development has nice concise information though it might be wise to add some more content. Also if the heading will be Glial dev, then neuron dev should be changed to Neuronal dev- for consistency. Adult function of ? However, this section is well-written! Concise and relevant- great work guys! Tissue Structure is starting to look good however needs more content. Really good student drawn image!! Though it might be good to be the image higher up on the page.
Molecular mechanisms/factors/genes has overall been well written. Perhaps a brief statement about what transcription factors are? Interesting image in abnormalities. I would personally appreciate an explanation of what I am seeing in the image. More discussion of a wider variety of abnormalities might be beneficial. Excellent coverage of animals models so far!! May be one more? Also, great use of images! Current research seems to be coming along well! Some formatting edits so that the video appears on the page would be good!
Overall, great work guys! Keep it up and move along with the project consistently! :) **
group contact information all contributions and group contact has been done through facebook messenger for the duration of this assignment History - empty, try and look for history of spinal nerve embryological discoveries as a potential starting point!
Embryonic Origins - good information, nice to read, but try and clean up the syntax just a little bit.
Developmental Process - Very clean and finished section with a thorough understanding of chemical mediators, overarching anatomy and embryological concepts.
Axonal Targeting - Try cleaning up the second sentence on Receptor Tyrosine Kinases, very good section otherwise.
Neuron Development - Good description of chemical mediators and their involvement in embryological processes
Glial Development - Also very good, as above. Consider adding a diagram or table, to simplify the knowledge into something your classmates can easily comprehend.
Adult Function - Could be mixed in with Tissue structure as one topic, otherwise both are good and set the stage of adult role & neurophysiology well.
Molecular Mechanisms - Fantastic all-round, not very much to fault, just try and preen up sentences here and there and proof-read, consider adding a little more information in a few sections, but only what would be necessary for the specific embryology.
Abnormalities, Animal Models and Current Research - All very well done, try simplifying some of the sentences occasionally.
References - Very good as well.
It would be good if there was a more fleshed out introduction, that outlined the purpose and scope of the project. The referencing is very comprehensive and shows that a lot of research has been put in. The information is presented will and is very in depth. In the Molecular Mechanisms section, I would suggest an introduction sentence or two, to tie the section together and help it to flow, overall it looks on track, but I would recommend having a think about the flow of the project and the layout of the information in terms of subheadings, an introduction would help to make that flow clear.
The references and images are great. I would like more of a description on the image in the neural crest migration to the DRG section as it seems brief and I am a little lost, maybe add information on what the colors are specifically so I know what I am looking at. That section is extremely well written with loads of information which is great. In the section glial development, the descriptions of the proteins and what they do would help me understand such as proteins SOX10 and P2x3 in the section there is information on the proteins but not specifically where they are form and what functions they have. The last thing is just fix up the glossary and history section and the project is complete and nicely done dorsal root ganglia group.
Z5112688 (talk) Overall the project is structured decently but I think the flow of the page could be improved. A clear introduction would help greatly as well as grammatical errors being fixed.History has been left unanswered. The Developmental process section is clear and concise. One improvement I can think of is to add some more images or other forms of media to make it more interesting rather then just text. For signalling pathway the information seems too brief, more explanation is needed. The image in abnormalities should have some text or something to explain its significance otherwise its hard for the readers to understand the purpose of the image. The animal models and current research section is really good. The information is interesting and relevant images have been used to further improve the educative purpose of the page. References is quite detailed showing a good amount of research being done on project.
Z5229549 (talk) 18:17, 6 October 2018 (AEST) Wiki page seems pretty much fleshed out with a decent number of diagrams. Some of the sections under development could be more concise. Introduction appears to be lacking as the history. The flow of the entire page however, needs touching up as it feels very choppy to read. Also, less technical jargon could be used to provide a more concise descript of some of the development sections.
Overall, a more or less complete page, disregarding the introduction and history. Well referenced with adequate visual aid.
Group 5, Dorsal Root Ganglion:
The introduction is currently very brief, but this is probably one of the final parts of the webpage that you will address.
It would be good if you had a little bit of information on the history of the Dorsal Root Ganglion/neural crest discovery.
I find the second paragraph of Embryonic Origins a little confusing - it is unclear whether you are saying that the DRG cells are already differentiated before migration, or whether this happens after. This process is better explained/repeated a little in the next section on development process. Maybe the Embryonic origins section should be simplified to just describing the location of the original neural crest cells, if migration is mentioned later anyway.
In “Tissue Structure” there are a few errors in writing that require addressing. I like your drawn diagram - it complements the paragraph’s description well. The placement of the image is slightly off, but this can be adjusted in your final edits.
Your Molecular mechanisms/factors/genes section is very thorough and clearly described. A figure showing the flow of events in the signalling pathway might be helpful to go along with this.
You need to reference your abnormality section, and edit its format a little, as currently the image seems out of place.
The animal models section is really interesting and well written, but it needs referencing at the start.
I’m impressed with the “Current Research” section - it is well written and the image is interesting and complements the paragraph.
You have used a broad range of references for this site, which shows you have done some extensive research on your topic.
This project shows a good understanding of the topic, with a good number of subheadings that will cover everything that needs to be discussed. The embryonic origin is a solid section with enough information. The developmental process has a lot of information and subheadings that includes all the necessary facts about the topic. There is a well-drawn student image, which shows enough detail. There is a good balance of image to text in the bottom half of the page. There is also a good number of examples for current research of the topic, as well as for the animal models, that really helps the reader’s understanding. There is a good number of references as well, which shows the depth of the research.
The introduction isn’t complete yet but can be left for the end of the project. The history has no information yet, so needs to be started. Sometimes it is difficult to tell whether a section of text is part of an overall section, or is a completely new section in itself. The top of the website does not have enough images in comparison to the amount of text. The images used in the abnormalities and at the beginning of the animal models do not have a description of what they are. The glossary list has not been formed yet either. The list of numbers at the beginning of the reference list is confusing.
For improvements, I think that more images should be added, and that could distinguish between the sections. The glossary also needs to be started. Maybe add a list of the abbreviations so the reader has a reference to go back to if they are confused. A video could also be added, maybe of the development or the molecular mechanisms.
Overall, this is a really good project with a lot of necessary information that the reader can utilise. There is also good images to support understanding and a good number of references that the reader can read themselves to gain more understanding if needed.
Z5229177 (talk) 16:46, 8 October 2018 (AEDT) For the history part, your group mentioned that there is a timeline of important figures who have made contributions big or small to dorsal root ganglion being discovered. However, in the section, only 1811 Charles Bell was mentioned. Is there supposed to be more content or more names and year being discussed in this section?
For the Embryonic Origins section, the idea of the content is there. I do feel if it would be more understandable and easier to see the overview of the neural crest cells differentiating into the different types of tissues with a suitable figure or image. As neural crest cells to dorsal root ganglion is the main focus of this project, it would be good to make this section clearer especially with a suitable image or video.
For the adult function section, I understand that the content written mainly discusses about how the dorsal root ganglia contributes to the different neurons and receptors in an adult CNS and spinal cord. However, I do not see any link of how it relates to “adult function” which is the title. What type of function is your group referring to here? Are there any examples? And hence can link to how these neurons contribute to that particular function? For example, how function of running relates to the neurons being used and the function of dorsal root ganglia in this case. Or maybe the title name can be changed instead?
Overall, most of the content is well-written and explained. Good use of the self-drawn image as well. Almost all of the sections are filled with content and the glossary and referencing has been done nicely as well. I feel that the project just need to touch up on some parts and it should be good enough!
- A good article for the overview of trunk neural crest cells
- Image about entire overview of neural crest migration
- find a image for the overview of DRG development
- if cannot find, use animal species to draw out the timeline
- work on chicken to identify origins of different components of DRG, neural crest
- timeline of discovery of DRG (use date of publication to put the timeline, around 1970s, original discovery is around 1930s)
- if cannot find about DRG, find about trunk neural crest migration to drg
- do they differentiate during migration or do they differentiate only when reaching the location
- which particular mechanism influence the differentiation process into DRG
- extension of DRG to different end points (epithelium, joints, muscle fibres)
- good to include a timeline (schwann cells -> differentiation and myelination)
- understanding schwann cell differentiation and myelination
- neuronal cell death (apoptosis if they do not reach the cell type)
- Differentiation process
- When they start to function
Tissue / Organ structure
Molecular Mechanisms / Factors / Genes
"Blocking of CXCR4 by morpholino or shRNA in premigratory chick trunk neural crest cells leads to significantly fewer cells that reach the dorsal aorta and instead populate the dorsal root ganglia"
- summary of signalling pathway and their interactions with each other
- identifying if molecular factors are growth or transcription factors
Abnormalities / Abnormal development
Dorsal Root Ganglionopathy is responsible for sensory impairment in CANVAS
"Sensory ganglionitis, variably called ganglionopathy, is a disease of sensory neurons in dorsal root ganglia. Major forms of these diseases are associated with neoplasm, Sjögren syndrome, and paraproteinemia or polyclonal gammopathy with or without known autoantibodies. Most cases follow subacute courses, but there are forms that develop chronically and acutely as well. Clinical signs seen include sensory ataxia exhibited by gait unsteadiness, a positive Romberg sign, reduced deep tendon reflexes, poor coordination, and pseudo-athetoid movements in the hands. Axonal degeneration warrants the treatment as early as possible. Early cases of immunologic origin that are immune-mediated may respond to plasmapheresis and immunosuppression. Differential diagnoses include environmental and industrial intoxication and adverse effects of antineoplastic and antibiotic drugs. The term “sensory neuronopathy” or “ganglionitis” refers to disorders of small neurons, larger neurons, and/or neurons of both sizes in the sensory ganglia."
"In zebrafish, trunk NCCs start migrating along a medial pathway in-between the somites and the NT. These NCCs align to and are affected by slow muscle cells in the middle part of the somite"
"Hedgehog (Hh) signal transduction is directly required in zebrafish DRG precursors for proper development of DRG neurons. Zebrafish mutations in the Hh signaling pathway result in the absence of DRG neurons and the loss of expression of neurogenin1 (ngn1), a gene required for determination of DRG precursors. Cell transplantation experiments demonstrate that Hh acts directly on DRG neuron precursors. Blocking Hh pathway activation at later stages of embryogenesis with the steroidal alkaloid, cyclopamine, further reveals that the requirement for a Hh signal response in DRG precursors correlates with the onset of ngn1 expression. These results suggest that Hh signaling may normally promote DRG development by regulating expression of ngn1 in DRG precursors."
Hedgehog signaling is directly required for the development of zebrafish dorsal root ganglia neurons. Josette M. Ungos, Rolf O. Karlstrom, David W. Raible. Development 2003 130: 5351-5362; doi: 10.1242/dev.00722
"Dorsal root ganglia (DRGs) arise from trunk neural crest cells that emerge from the dorsal neuroepithelium and coalesce into segmental streams that migrate ventrally along the developing somites. Proper formation of DRGs involves not only normal trunk neural crest migration, but also the ability of DRG progenitors to pause at a particular target location where they can receive DRG-promoting signals. In mammalian embryos, a receptor tyrosine kinase proto-oncogene, ErbB3, is required for proper trunk neural crest migration. Here, we show that in zebrafish mutants lacking ErbB3 function, neural crest cells do not pause at the location where DRGs normally form and DRG neurons are not generated. We also show that these mutants lack trunk neural crest-derived sympathetic neurons, but that cranial neural crest-derived enteric neurons appear normal. We isolated three genes encoding neuregulins, ErbB3 ligands, and show that two neuregulins function together in zebrafish trunk neural crest cell migration and in DRG formation. Together, our results suggest that ErbB3 signaling is required for normal migration of trunk, but not cranial, neural crest cells."
"cdon is expressed in developing premigratory NCCs but is downregulated once the cells become migratory. Knockdown of cdon results in aberrant migration of trunk NCCs: crestin positive cells can emigrate out of the neural tube but stall shortly after the initiation of migration. Live cell imaging analysis demonstrates reduced directedness of migration, increased velocity and mispositioned cell protrusions. In addition, transplantation analysis suggests that cdon is required cell-autonomously for directed NCC migration in the trunk."
Powell DR, Williams JS, Hernandez-Lagunas L, Salcedo E, O'Brien JH & Artinger KB. (2015). Cdon promotes neural crest migration by regulating N-cadherin localization. Dev. Biol. , 407, 289-99. PMID: 26256768 DOI.
Current research (Labs)
- Brief, clear and concise
- HAVE A LIST OF ACRONYMS TO CONDENSE THE INFORMATION