Mouse Thymus Movie 1: Difference between revisions

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Embryology - 6 May 2024 Expand to Translate
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Adult Mouse Thymus - T cell Elimination

<html5media height="380" width="400">File:Mouse_adult_lymph_node_01.mp4</html5media>

Click Here to play on mobile device

Dying thymocytes are efficiently cleared by phagocytes.

Examples of cell death in situ during negative selection. Purified CD4+CD8+ F5 thymocytes labeled with SNARF and Hoechst were introduced into LysM-GFP thymic slices then treated with 1 nM specific peptide for 30 min.

The incubation was continued for the indicated times and the slices were imaged by time-lapse two-photon microscopy.

The arrowheads point to dying thymocytes.

Scale bars are 5 µm.

Reference

<pubmed>23700386</pubmed>| PLoS Biol. | PLoS Biol. Immunobiology Collection

Copyright

© 2013 Dzhagalov et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

doi:10.1371/journal.pbio.1001566.s006 (MOV)

http://journals.plos.org/plosbiology/article/asset?unique&id=info:doi/10.1371/journal.pbio.1001566.s006

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Cite this page: Hill, M.A. (2024, May 6) Embryology Mouse Thymus Movie 1. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Thymus_Movie_1

What Links Here?

@@ Line 1: / Line 1: @@
 {{Movie header}}
-==Adult Mouse Lymph Node - T cell motility and transendothelial migration==
+==Adult Mouse Thymus - T cell Elimination==
 {|
 |-
@@ Line 7: / Line 7: @@
 [[Media:Mouse_adult_lymph_node_01.mp4|'''Click Here''' to play on mobile device]]
-| Examples of cell death in situ during negative selection. Purified CD4+CD8+ F5 thymocytes labeled with SNARF and Hoechst were introduced into LysM-GFP thymic slices then treated with 1 nM specific peptide for 30 min. The incubation was continued for the indicated times and the slices were imaged by time-lapse two-photon microscopy. The arrowheads point to dying thymocytes.
+| Dying thymocytes are efficiently cleared by phagocytes.
+Examples of cell death in situ during negative selection. Purified CD4+CD8+ F5 thymocytes labeled with SNARF and Hoechst were introduced into LysM-GFP thymic slices then treated with 1 nM specific peptide for 30 min.
+The incubation was continued for the indicated times and the slices were imaged by time-lapse two-photon microscopy.
+The arrowheads point to dying thymocytes.
 Scale bars are 5 µm.