File:Sheep oocyte 02.jpg

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Sheep Oocyte Distribution of TERT

An in vitro grown early antral follicle with a not surrounded nucleolus (NSN) chromatin configuration which localized TERT in the nucleus.

  • TERT - Red (Cy3-conjugated secondary antibody) (telomerase reverse transcriptase, TERT)
  • DNA - Green (SYBR Green 14/I)


Sheep Oocyte TERT: preantral | early antral | early antral | preovulatory follicle | Oocyte Development | Sheep Development

Reference

Barboni B, Russo V, Cecconi S, Curini V, Colosimo A, Garofalo ML, Capacchietti G, Di Giacinto O & Mattioli M. (2011). In vitro grown sheep preantral follicles yield oocytes with normal nuclear-epigenetic maturation. PLoS ONE , 6, e27550. PMID: 22132111 DOI.


Copyright

© 2011 Barboni et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Figure 7. doi:info:doi/10.1371/journal.pone.0027550.g007

journal.pone.0027550.g007.jpg

Cropped, resized and relabelled from full figure.

Examples of oocyte TERT distribution, visualized with Cy3-conjugated secondary antibody (red), while DNA was counterstained in green with SYBR Green 14/I. TERT sub-cellular localization was influenced by chromatin configuration in all in vitro and in vivo classes of germ cells analyzed. (A,B) Oocytes derived from a preantral (PA) follicle (A) and an in vitro grown early antral (EA) follicle (B) with a not surrounded nucleolus (NSN) chromatin configuration which localized TERT in the nucleus. (C) in vitro grown oocyte derived from an EA follicle with a surrounded nucleolus (SN) chromatin configuration which reveals TERT between the nucleus and ooplasm. (D) Oocyte of a preovulatory follicle with condensed chromatin localized surrounding the nucleolus and the nuclear envelope (SNE configuration) and with a clear subcortical and perinuclear cytoplasmatic TERT distribution. Scale bar = 25 µm.


Cite this page: Hill, M.A. (2024, June 18) Embryology Sheep oocyte 02.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Sheep_oocyte_02.jpg

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current02:20, 7 June 2012Thumbnail for version as of 02:20, 7 June 2012650 × 651 (59 KB)Z8600021 (talk | contribs)==Sheep Oocyte Distribution of TERT== TERT - telomerase catalytic subunit. Sheep oocyte 02.jpg {{Sheep Oocyte TERT}} ===Reference=== <pubmed>22132111</pubmed>| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027550 PLoS One.] Co

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