File:Mouse - rhombomere boundaries.jpg
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Cell clones respect the rhombomeric boundaries upon early labelling
(A) Scheme depicting the embryonic stage upon TM administration (E5.5 dpc), the embryonic stage of Cre-induction (E6.0 dpc), and the stage when the observation was carried out and the hindbrain dissected (E9.5 dpc). Note the dramatic change in embryo morphology between the time of TM administration and the time of embryo observation. Blue areas in 5.5–6.5 dpc depict the embryonic tissue. r1-r7, rhombomere 1–7.
(B) Immunostaining to reveal GFP-positive clones in whole embryos (in toto) and in flat-mounted hindbrains.
Tamoxifen (TM) administration Cre-mediated recombination is induced and cells are genetically marked, therefore labelling does not dilute with cell division and growth.
- Links: rhombencephalon | mouse
Reference
Jimenez-Guri E, Udina F, Colas JF, Sharpe J, Padrón-Barthe L, Torres M & Pujades C. (2010). Clonal analysis in mice underlines the importance of rhombomeric boundaries in cell movement restriction during hindbrain segmentation. PLoS ONE , 5, e10112. PMID: 20404937 DOI.
Copyright
© 2010 Jimenez-Guri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Original file name:Figure 1. Journal.pone.0010112.g001.jpg
Cite this page: Hill, M.A. (2024, April 27) Embryology Mouse - rhombomere boundaries.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Mouse_-_rhombomere_boundaries.jpg
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
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| current | 12:40, 8 November 2010 | | 669 × 1,000 (147 KB) | S8600021 (talk | contribs) | ==Cell clones respect the rhombomeric boundaries upon early labelling== (A) Scheme depicting the embryonic stage upon TM administration (5.5 dpc), the embryonic stage of Cre-induction (6.0 dpc), and the stage when the observation was carried out and the |
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