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==Liver Polyploidy== | ==Liver Polyploidy== | ||
Polyploid hepatocyte mitoses with multipolar spindles and chromosome segregation defects | Polyploid hepatocyte mitoses ''in vitro'' with multipolar spindles and chromosome segregation defects. | ||
* '''A''' - bipolar spindles were established and maintained by centrosome clustering (in about half of tetraploid mitoses) | |||
* '''B C''' - centrosomes oriented on 3-4 distinct poles (reflecting either true multipolar spindles or alignment of prometaphase chromosomes from binucleated hepatocytes) | |||
* '''D''' - octaploid hepatocytes established multipolar spindles with as many as 8 poles | |||
* '''E''' - about 1% of cells in anaphase or telophase were oriented with tripolar spindles | |||
* '''F''' - hepatocytes with 2 discrete mitotic spindles synchronized in metaphase | |||
* '''G''' - hepatocytes with 2 discrete mitotic spindles synchronized in anaphase | |||
* '''I - L''' - mitotic figures similar to those seen in cultured hepatocytes were also seen during hepatocyte proliferation ''in vivo'' | |||
See [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2967727/?tool=pubmed#S21 supplementary movies] for recordings of mitotic divisions. | |||
===Methods=== | |||
* cells were fixed with methanol | |||
* Primary antibodies for alpha-tubulin (clone DM1A, Sigma) | |||
* Primary antibodies were detected with species-specific secondaries conjugated to Alexafluor 488 or Alexafluor 555 (Invitrogen). | |||
* Nuclei were stained with 40 μg/ml Hoechst 33342 (Invitrogen). | |||
Original File Name: Fig. 3 Nature09414-f3.2.jpg | Original File Name: Fig. 3 Nature09414-f3.2.jpg | ||
===Reference=== | |||
{{#pmid:20861837}} | |||
====Copyright==== | |||
Reprinted by permission from Macmillan Publishers Ltd. | |||
http://www.nature.com/authors/policies/license.html | http://www.nature.com/authors/policies/license.html | ||
{{Footer}} | |||
[[Category:Mouse]] [[Category:Mitosis]] [[Category:Liver]] [[Category:Histology]] |
Latest revision as of 12:41, 25 February 2019
Liver Polyploidy
Polyploid hepatocyte mitoses in vitro with multipolar spindles and chromosome segregation defects.
- A - bipolar spindles were established and maintained by centrosome clustering (in about half of tetraploid mitoses)
- B C - centrosomes oriented on 3-4 distinct poles (reflecting either true multipolar spindles or alignment of prometaphase chromosomes from binucleated hepatocytes)
- D - octaploid hepatocytes established multipolar spindles with as many as 8 poles
- E - about 1% of cells in anaphase or telophase were oriented with tripolar spindles
- F - hepatocytes with 2 discrete mitotic spindles synchronized in metaphase
- G - hepatocytes with 2 discrete mitotic spindles synchronized in anaphase
- I - L - mitotic figures similar to those seen in cultured hepatocytes were also seen during hepatocyte proliferation in vivo
See supplementary movies for recordings of mitotic divisions.
Methods
- cells were fixed with methanol
- Primary antibodies for alpha-tubulin (clone DM1A, Sigma)
- Primary antibodies were detected with species-specific secondaries conjugated to Alexafluor 488 or Alexafluor 555 (Invitrogen).
- Nuclei were stained with 40 μg/ml Hoechst 33342 (Invitrogen).
Original File Name: Fig. 3 Nature09414-f3.2.jpg
Reference
Duncan AW, Taylor MH, Hickey RD, Hanlon Newell AE, Lenzi ML, Olson SB, Finegold MJ & Grompe M. (2010). The ploidy conveyor of mature hepatocytes as a source of genetic variation. Nature , 467, 707-10. PMID: 20861837 DOI.
Copyright
Reprinted by permission from Macmillan Publishers Ltd.
http://www.nature.com/authors/policies/license.html
Cite this page: Hill, M.A. (2024, May 19) Embryology Liver polyploidy 01.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Liver_polyploidy_01.jpg
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
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