File:Human fetal ovary FOXL2.jpg: Difference between revisions

Latest revision as of 10:51, 27 February 2018

Human Fetal Ovary FOXL2

Immunolocalization of FOXL2 in the human fetal ovary.

A and B - 14 weeks (GA).
C and D - 19 weeks (GA).

Positive immunofluorescence for FOXL2 is green, counterstain is propidium iodide (red), hence nuclear FOXL2 is yellow. Inset in (A) is negative control.

E and F - Double immunofluorescence for FOXL2 (green) and OCT4 (red) in human fetal ovary showing absence of colocalization, with FOXL2 expressed by somatic cells and OCT4 by germ cells.
- E - 9 weeks (GA).
- F - 19 weeks (GA).

Legend

Blue - DAPI nuclear stain
G - germ cell
S - somatic cell.

Scale bars are 50 µm (A, applies to E and F) or 10 µm (B, C, D).

Links: Ovary Development

Reference

Duffin K, Bayne RA, Childs AJ, Collins C & Anderson RA. (2009). The forkhead transcription factor FOXL2 is expressed in somatic cells of the human ovary prior to follicle formation. Mol. Hum. Reprod. , 15, 771-7. PMID: 19706741 DOI.

Citation: The forkhead transcription factor FOXL2 is expressed in somatic cells of the human ovary prior to follicle formation. Duffin K, Bayne RA, Childs AJ, Collins C, Anderson RA. Mol Hum Reprod. 2009 Dec;15(12):771-7. Epub 2009 Aug 25.

"In summary, the expression of FOXL2 in somatic cells of the developing human ovary before and during follicle formation supports a conserved and continuing role for this factor in somatic/germ cell interactions from the earliest stages of human ovarian development."

FOXL2 gene are associated with syndromic and non-syndromic ovarian failure.
Mutations in FOXL2 result in blepharophimosis/ptosis/epicanthus inversus syndrome (BPES).
FOXL2 is a member of the winged helix/forkhead transcription factor family (39 known members) variety of functions acting as transcriptional activators and repressors.

Copyright

Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Original File name: Figure 2 - gap06502.jpeg

Cite this page: Hill, M.A. (2024, May 7) Embryology Human fetal ovary FOXL2.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Human_fetal_ovary_FOXL2.jpg

What Links Here?

File history

Click on a date/time to view the file as it appeared at that time.

	Date/Time	Thumbnail	Dimensions	User	Comment
current	10:06, 3 May 2010		739 × 1,087 (300 KB)	S8600021 (talk \| contribs)	Immunolocalization of FOXL2 in the human fetal ovary. A and B, 14 weeks; C and D, 19 weeks. Positive immunofluorescence for FOXL2 is green, counterstain is propidium iodide (red), hence nuclear FOXL2 is yellow. Inset in (A) is negative control. (E, F)

You cannot overwrite this file.

File usage

The following 3 pages use this file:

@@ Line 1: / Line 1: @@
+==Human Fetal Ovary FOXL2==
 Immunolocalization of FOXL2 in the human fetal ovary.
-A and B, 14 weeks; C and D, 19 weeks. Positive immunofluorescence for FOXL2 is green, counterstain is propidium iodide (red), hence nuclear FOXL2 is yellow. Inset in (A) is negative control.
+* '''A and B''' - 14 weeks (GA).
+* '''C and D''' - 19 weeks (GA).
+Positive immunofluorescence for FOXL2 is green, counterstain is propidium iodide (red), hence nuclear FOXL2 is yellow. Inset in (A) is negative control.
-(E, F) Double immunofluorescence for FOXL2 (green) and OCT4 (red) in human fetal ovary showing absence of colocalization, with FOXL2 expressed by somatic cells and OCT4 by germ cells. E, 9 weeks gestation; F, 19 weeks.
+* '''E and F''' - Double immunofluorescence for FOXL2 (green) and OCT4 (red) in human fetal ovary showing absence of colocalization, with FOXL2 expressed by somatic cells and OCT4 by germ cells.
+** E - 9 weeks (GA).
+** F - 19 weeks (GA).
-Blue nuclear stain is DAPI. G: germ cell, S: somatic cell.
+===Legend===
+* Blue - DAPI nuclear stain
+* G - germ cell
+* S -  somatic cell.
 Scale bars are 50 µm (A, applies to E and F) or 10 µm (B, C, D).
-Original File name: Figure 2  - gap06502.jpeg
+:'''Links:''' [[Ovary Development]]
+===Reference===
+{{#pmid:19706741}}
-Citation: The forkhead transcription factor FOXL2 is expressed in somatic cells of the human ovary prior to follicle formation. Duffin K, Bayne RA, Childs AJ, Collins C, Anderson RA. Mol Hum Reprod. 2009 Dec;15(12):771-7. Epub 2009 Aug 25. [http://www.ncbi.nlm.nih.gov/pubmed/19706741 PMID: 19706741] | [http://molehr.oxfordjournals.org/cgi/content/full/15/12/771?view=long&pmid=19706741 Mol Hum Reprod.]
+Citation: The forkhead transcription factor FOXL2 is expressed in somatic cells of the human ovary prior to follicle formation. Duffin K, Bayne RA, Childs AJ, Collins C, Anderson RA. Mol Hum Reprod. 2009 Dec;15(12):771-7. Epub 2009 Aug 25.
 :"In summary, the expression of FOXL2 in somatic cells of the developing human ovary before and during follicle formation supports a conserved and continuing role for this factor in somatic/germ cell interactions from the earliest stages of human ovarian development."
@@ Line 20: / Line 34: @@
 * FOXL2 is a member of the winged helix/forkhead transcription factor family (39 known members) variety of functions acting as transcriptional activators and repressors.
+====Copyright====
 © The Author 2009.
@@ Line 25: / Line 40: @@
 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
+Original File name: Figure 2  - gap06502.jpeg
+{{Footer}}
 [[Category:Ovary]]