File:Human-spermatozoa.jpg: Difference between revisions

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Human Spermatozoa

Human spermatozoa viewed under brightfield phase contrast optics microscopy. Note the general morphology and size of the spermatozoa head and tail.

Reference

<pubmed>19582168</pubmed>| [1]

Copyright

© 2009 De Blas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Original file name: Figure 1. TRPM8 channels are present in human sperm. (Image from: panel B)

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	Date/Time	Thumbnail	Dimensions	User	Comment
current	22:27, 2 May 2010		600 × 581 (19 KB)	S8600021 (talk \| contribs)	Human spermatozoa Image from: panel B Figure 1. TRPM8 channels are present in human sperm. A) RT-PCR showing a TRPM8 fragment amplified from human semen. Total RNA was extracted from human semen and cDNA was prepared. Specific oligonucleotides were use

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 ==Human Spermatozoa==
-A brightfield image using phase optics of human spermatozoa.
+Human spermatozoa viewed under brightfield phase contrast optics microscopy. Note the general morphology and size of the spermatozoa head and tail.
+{{Spermatozoa images}}
-Original file name: Figure 1. TRPM8 channels are present in human sperm. (Image from: panel B)
+===Reference===
-A) RT-PCR showing a TRPM8 fragment amplified from human semen. Total RNA was extracted from human semen and cDNA was prepared. Specific oligonucleotides were used for TRPM8. Identity of the expected size (in white numbers) fragment was confirmed by DNA sequencing. B) Immunocytochemistry showing the presence of TRPM8 in heads and flagella of human sperm. Left panels show the confocal fluorescent images with pseudocolor and right panels show their corresponding phase contrast images. C) Western blot experiments showing TRPM8 reactivity (~130 kDa) in sperm lysates (anti TRPM8 from Gene Tex). n≥3.
-==Reference==
 <pubmed>19582168</pubmed>| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0006095]
+====Copyright====
-Citation: De Blas GA, Darszon A, Ocampo AY, Serrano CJ, Castellano LE, et al. (2009) TRPM8, a Versatile Channel in Human Sperm. PLoS ONE 4(6): e6095. doi:10.1371/journal.pone.0006095
+© 2009 De Blas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
-http://www.ncbi.nlm.nih.gov/pubmed/19582168
-http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0006095
-Editor: Wen-Liang Zhou, Sun Yat-Sen University, China
-Received: March 31, 2009; Accepted: May 30, 2009; Published: June 30, 2009
-Copyright: © 2009 De Blas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
+Original file name: Figure 1. TRPM8 channels are present in human sperm. (Image from: panel B)
 [[Category:Human]] [[Category:Spermatozoa]]