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(Human spermatozoa Image from: panel B Figure 1. TRPM8 channels are present in human sperm. A) RT-PCR showing a TRPM8 fragment amplified from human semen. Total RNA was extracted from human semen and cDNA was prepared. Specific oligonucleotides were use)
 
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Human spermatozoa
==Human Spermatozoa==


Image from: panel B
Human spermatozoa viewed under brightfield phase contrast optics microscopy. Note the general morphology and size of the spermatozoa head and tail.


Figure 1. TRPM8 channels are present in human sperm.


A) RT-PCR showing a TRPM8 fragment amplified from human semen. Total RNA was extracted from human semen and cDNA was prepared. Specific oligonucleotides were used for TRPM8. Identity of the expected size (in white numbers) fragment was confirmed by DNA sequencing. B) Immunocytochemistry showing the presence of TRPM8 in heads and flagella of human sperm. Left panels show the confocal fluorescent images with pseudocolor and right panels show their corresponding phase contrast images. C) Western blot experiments showing TRPM8 reactivity (~130 kDa) in sperm lysates (anti TRPM8 from Gene Tex). n≥3.
{{Spermatozoa images}}


===Reference===


Citation: De Blas GA, Darszon A, Ocampo AY, Serrano CJ, Castellano LE, et al. (2009) TRPM8, a Versatile Channel in Human Sperm. PLoS ONE 4(6): e6095. doi:10.1371/journal.pone.0006095
<pubmed>19582168</pubmed>| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0006095]


http://www.ncbi.nlm.nih.gov/pubmed/19582168


http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0006095
====Copyright====


Editor: Wen-Liang Zhou, Sun Yat-Sen University, China
© 2009 De Blas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Received: March 31, 2009; Accepted: May 30, 2009; Published: June 30, 2009
Original file name: Figure 1. TRPM8 channels are present in human sperm. (Image from: panel B)


Copyright: © 2009 De Blas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
[[Category:Human]] [[Category:Spermatozoa]]
 
 
[[Category:Spermatozoa]]

Latest revision as of 15:34, 5 May 2013

Human Spermatozoa

Human spermatozoa viewed under brightfield phase contrast optics microscopy. Note the general morphology and size of the spermatozoa head and tail.


Spermatozoa Images: Spermatozoa BF | Spermatozoon BF | Spermatozoon EM | Spermatozoon EM | Historic drawing | Category:Spermatozoa | Spermatozoa Development | Testis Development

Reference

<pubmed>19582168</pubmed>| [1]


Copyright

© 2009 De Blas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Original file name: Figure 1. TRPM8 channels are present in human sperm. (Image from: panel B)

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