File:Detached-cell method of transplanting nuclei.jpg
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Detached-cell method of transplanting nuclei
- A - A single donor cell is sucked into a micropipette.
- B - The air bubble marker moves sufficiently far up the pipette shaft to serve as a guide when the pipette tip and donor cell have been immersed in the recipient egg.
Attached-cell method of transplanting nuclei.
C, D - The edges of a well-spread cell are cut with a fine glass needle. E, F - The cytoplasm spreads evenly round the nucleus, and sticks to the glass needle. G, H - The cell (with an incomplete cell membrane) is sucked from the needle into a wide-bore micropipette, and is then injected into a recipient egg in the usual way.
Reference
<pubmed>4923666</pubmed>| Development | PDF
http://dev.biologists.org/site/misc/rights_permissions.xhtml
Fig. 1 for educational use only
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 12:17, 11 October 2012 | | 566 × 800 (93 KB) | Z8600021 (talk | contribs) | ==Detached-cell method of transplanting nuclei== * A - A single donor cell is sucked into a micropipette. * B - The air bubble marker moves sufficiently far up the pipette shaft to serve as a guide when the pipette tip and donor cell have been immersed |
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