File:Chicken- limb bud chondrogenesis.jpg: Difference between revisions
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'''Links:''' [[Chicken Development]] | [[Wnt]] | [[Musculoskeletal_System_-_Limb_Development|Limb Development]] | [[Musculoskeletal_System_-_Cartilage_Development|Cartilage Development]] | |||
Revision as of 15:08, 21 October 2010
Chicken Limb Bud Chondrogenesis
In situ hybridizations of chicken wing buds with probes against the chondrogenic markers Sox9 (A, B) and Coll2a (C, D), after removal of the dorsal ectoderm at HH-stage 20-21 and 24 or 48 h reincubation.
On the left side is the control limb, the right limb has been manipulated. Arrowheads indicate the site of ectoderm removal. A Expression of Sox9 after 24 h. Expression is stronger on the operated side.
A Transverse section of the control limb showing low Sox9 expression.
- a Transverse section of the control limb showing normal low Sox9 expression.
- a' Transverse section of the operated limb showing strong Sox9 expression, which is extending ectopically towards the dorsal surface of the limb.
B Expression of Sox9 after 48 h. Expression is stronger on the operated side.
- b Transverse section of the control limb showing normal Sox9 expression.
- b' Transverse section of the operated limb showing enhanced Sox9 expression, which is extending ectopically towards the dorsal surface of the limb.
C Expression of ColIIA after 24 h. Expression is stronger on the operated side. Dislocated fragments of gold foil are still visible.
- c Transverse section of the control limb showing low ColIIA expression.
- c' Transverse section of the operated limb showing strong ColIIA expression, which is extending ectopically towards the dorsal surface of the limb.
D Expression of ColIIA after 48 h. Expression is stronger on the operated side.
- d Transverse section of the control limb showing normal ColIIA expression.
- d' Transverse section of the operated limb showing enhanced ColIIA expression, which is extending ectopically towards the dorsal surface of the limb.
Scale bar 500 μm.
Links: Chicken Development | Wnt | Limb Development | Cartilage Development
Original File name: 1471-213X-10-32-1.jpg
Reference
<pubmed>20334703</pubmed>| BMC
Geetha-Loganathan et al. BMC Developmental Biology 2010 10:32 doi:10.1186/1471-213X-10-32 Ectodermal Wnt6 is an early negative regulator of limb chondrogenesis in the chicken embryo. Geetha-Loganathan P, Nimmagadda S, Christ B, Huang R, Scaal M. BMC Dev Biol. 2010 Mar 25;10(1):32. PMID: 20334703 | BMC
© 2010 Geetha-Loganathan et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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current | 08:17, 28 April 2010 | 600 × 1,032 (163 KB) | S8600021 (talk | contribs) | Chicken- limb bud chondrogenesis In situ hybridizations of chicken wing buds with probes against the chondrogenic markers Sox9 (A, B) and Coll2a (C, D), after removal of the dorsal ectoderm at HH-stage 20-21 and 24 or 48 h reincubation. On the left sid |
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