File:Chicken- limb bud chondrogenesis.jpg

From Embryology

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Chicken Limb Bud Chondrogenesis

In situ hybridizations of chicken wing buds with probes against the chondrogenic markers Sox9 (A, B) and Coll2a (C, D), after removal of the dorsal ectoderm at HH-stage 20-21 and 24 or 48 h reincubation.

On the left side is the control limb, the right limb has been manipulated. Arrowheads indicate the site of ectoderm removal. A Expression of Sox9 after 24 h. Expression is stronger on the operated side.

A Transverse section of the control limb showing low Sox9 expression.

  • a Transverse section of the control limb showing normal low Sox9 expression.
  • a' Transverse section of the operated limb showing strong Sox9 expression, which is extending ectopically towards the dorsal surface of the limb.

B Expression of Sox9 after 48 h. Expression is stronger on the operated side.

  • b Transverse section of the control limb showing normal Sox9 expression.
  • b' Transverse section of the operated limb showing enhanced Sox9 expression, which is extending ectopically towards the dorsal surface of the limb.

C Expression of ColIIA after 24 h. Expression is stronger on the operated side. Dislocated fragments of gold foil are still visible.

  • c Transverse section of the control limb showing low ColIIA expression.
  • c' Transverse section of the operated limb showing strong ColIIA expression, which is extending ectopically towards the dorsal surface of the limb.

D Expression of ColIIA after 48 h. Expression is stronger on the operated side.

  • d Transverse section of the control limb showing normal ColIIA expression.
  • d' Transverse section of the operated limb showing enhanced ColIIA expression, which is extending ectopically towards the dorsal surface of the limb.


Scale bar 500 μm.


Links: Chicken Development | Limb Development | Cartilage Development


Original File name: 1471-213X-10-32-1.jpg

Reference

Geetha-Loganathan P, Nimmagadda S, Christ B, Huang R & Scaal M. (2010). Ectodermal Wnt6 is an early negative regulator of limb chondrogenesis in the chicken embryo. BMC Dev. Biol. , 10, 32. PMID: 20334703 DOI.

Copyright

© 2010 Geetha-Loganathan et al; licensee BioMed Central Ltd.


This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Cite this page: Hill, M.A. (2024, May 23) Embryology Chicken- limb bud chondrogenesis.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Chicken-_limb_bud_chondrogenesis.jpg

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G

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current08:17, 28 April 2010Thumbnail for version as of 08:17, 28 April 2010600 × 1,032 (163 KB)S8600021 (talk | contribs)Chicken- limb bud chondrogenesis In situ hybridizations of chicken wing buds with probes against the chondrogenic markers Sox9 (A, B) and Coll2a (C, D), after removal of the dorsal ectoderm at HH-stage 20-21 and 24 or 48 h reincubation. On the left sid