File:Canonical Wnt signaling in Wnt5a KO limb buds.jpg

Canonical Wnt signaling was increased in the Wnt-5a-/- limb buds.

Gene expression in embryos was examined by whole mount in situ hybridization.

(A) At 12.5 dpc, the expression of Sox9, which marks early chondrogenesis, was not detected in the distal-most limb bud of the Wnt-5a-/- limb (arrow).

(B) Ectopic LacZ staining (arrow) was detected in the Wnt-5a-/-/TOPGAL distal limb at 12.5 dpc. (C) The ß-catenin protein level was increased in the distal Wnt-5a-/- limb at 11.5 dpc. (D) Sfrp-2 expressing cells (red), but not control cells (blue), induced ectopic expression of ColII (arrows), a marker for chondrocyte differentiation in both wild-type and Wnt-5a-/- limbs.

Original File name: 200303158f5.jpeg

Figure 5. http://jcb.rupress.org/content/vol162/issue5/images/large/200303158f5.jpeg

Wnt-5a inhibits the canonical Wnt pathway by promoting GSK-3-independent beta-catenin degradation. Topol L, Jiang X, Choi H, Garrett-Beal L, Carolan PJ, Yang Y. J Cell Biol. 2003 Sep 1;162(5):899-908. PMID: 12952940

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