Talk:Somatic Cell Nuclear Transfer
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Cite this page: Hill, M.A. (2026, September 11) Embryology Somatic Cell Nuclear Transfer. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Somatic_Cell_Nuclear_Transfer |
2012
Lessons learned from cloning dogs
Reprod Domest Anim. 2012 Aug;47 Suppl 4:115-9. doi: 10.1111/j.1439-0531.2012.02064.x.
Kim M, Oh H, Kim G, Park J, Park E, Jang G, Ra J, Kang S, Lee B. Source Department of Theriogenology and Biotechnology, Seoul National University, Seoul, Korea Stem Cell Research Center, RNL BIO, Seoul, Korea.
Abstract
The aim of this article is to review dog cloning research and to suggest its applications based on a discussion about the normality of cloned dogs. Somatic cell nuclear transfer was successfully used for production of viable cloned puppies despite limited understanding of in vitro dog embryo production. Cloned dogs have similar growth characteristics to those born from natural fertilization, with no evidence of serious adverse effects. The offspring of cloned dogs also have similar growth performance and health to those of naturally bred puppies. Therefore, cloning in domestic dogs can be applied as an assisted reproductive technique to conserve endangered species, to treat sterile canids or aged dogs, to improve reproductive performance of valuable individuals and to generate disease model animals. © 2012 Blackwell Verlag GmbH.
PMID 22827359
Segregation of donor cell mitochondrial DNA in gaur-bovine interspecies somatic cell nuclear transfer embryos, fetuses and an offspring
Mitochondrion. 2012 Jul 21;12(5):506-513. [Epub ahead of print]
Imsoonthornruksa S, Srirattana K, Phewsoi W, Tunwattana W, Parnpai R, Ketudat-Cairns M. Source Embryo Technology and Stem Cell Research Center, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Abstract
The fate of foreign mitochondrial DNA (mtDNA) following somatic cell nuclear transfer (SCNT) is still controversial. In this study, we examined the transmission of the heteroplasmic mtDNA of gaur donor cells and recipient bovine oocytes to an offspring and aborted and mummified fetuses at various levels during the development of gaur-bovine interspecies SCNT (iSCNT) embryos. High levels of the donor cell mtDNA were found in various tissue samples but they did not have any beneficial effect to the survival of iSCNT offspring. However, the factors on mtDNA inheritance are unique for each iSCNT experiment and depend on the recipient oocyte and donor cell used, which might play an important role in the efficiency of iSCNT. Copyright © 2012 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
PMID 22824460
Improved cloning efficiency and developmental potential in bovine somatic cell nuclear transfer with the oosight imaging system
Cell Reprogram. 2012 Aug;14(4):305-11. Epub 2012 Jul 20.
Kim EY, Park MJ, Park HY, Noh EJ, Noh EH, Park KS, Lee JB, Jeong CJ, Riu KZ, Park SP. Source 1 Mirae Biotech, Seoul 143-854, Korea. Abstract Abstract In somatic cell nuclear transfer (SCNT) procedures, exquisite enucleation of the recipient oocyte is critical to cloning efficiency. The purpose of this study was to compare the effects of two enucleation systems, Hoechst staining and UV irradiation (hereafter, irradiation group) and Oosight imaging (hereafter, Oosight group), on the in vitro production of bovine SCNT embryos. In the Oosight group, the apoptotic index (2.8±0.5 vs. 7.3±1.2) was lower, and the fusion rate (75.6% vs. 62.9%), cleavage rate (78.0% vs. 63.7%), blastocyst rate (40.2% vs. 29.2%), and total cell number (128.3±4.8 vs. 112.2±7.6) were higher than those in the irradiation group (all p<0.05). The overall efficiency after SCNT was twice as high in the Oosight group as that in the irradiation group (p<0.05). The relative mRNA expression levels of Oct4, Nanog, Interferon-tau, and Dnmt3A were higher and those of Caspase-3 and Hsp70 were lower in the Oosight group compared with the irradiation group (p<0.05). This is the first report to show the positive effect of the Oosight imaging system on molecular gene expression in the SCNT embryo. The Oosight imaging system may become the preferred choice for enucleation because it is less detrimental to the developmental potential of bovine SCNT embryos.
PMID 22816525
Inheritance of mitochondrial DNA in serially recloned pigs by somatic cell nuclear transfer (SCNT)
Biochem Biophys Res Commun. 2012 Aug 10;424(4):765-70. Epub 2012 Jul 15.
Do M, Jang WG, Hwang JH, Jang H, Kim EJ, Jeong EJ, Shim H, Hwang SS, Oh KB, Byun SJ, Kim JH, Lee JW. Source Regenerative Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305 806, Republic of Korea.
Abstract
Somatic cell nuclear transfer (SCNT) has been established for the transmission of specific nuclear DNA. However, the fate of donor mitochondrial DNA (mtDNA) remains unclear. Here, we examined the fate of donor mtDNA in recloned pigs through third generations. Fibroblasts of recloned pigs were obtained from offspring of each generation produced by fusion of cultured fibroblasts from a Minnesota miniature pig (MMP) into enucleated oocytes of a Landrace pig. The D-loop regions from the mtDNA of donor and recipient differ at nucleotide sequence positions 16050 (A→T), 16062 (T→C), and 16135 (G→A). In order to determine the fate of donor mtDNA in recloned pigs, we analyzed the D-loop region of the donor's mtDNA by allele-specific PCR (AS-PCR) and real-time PCR. Donor mtDNA was successfully detected in all recloned offspring (F1, F2, and F3). These results indicate that heteroplasmy that originate from donor and recipient mtDNA is maintained in recloned pigs, resulting from SCNT, unlike natural reproduction. Copyright © 2012 Elsevier Inc. All rights reserved. PMID 22809505
