File:WNT3A transfer via filopodia.png

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Revision as of 11:31, 12 April 2019 by Z8600021 (talk | contribs) (==WNT3A transfer via filopodia== (a-c) Fluorescence photomicrographs of adjacent HeLa cells expressing RUSH-eGFP-WNT3A at indicated time points after biotin addition. From S7 video. (a) WNT3A is in the Golgi after biotin treatment. Inset showing WNT3A...)
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Summary

WNT3A transfer via filopodia

(a-c) Fluorescence photomicrographs of adjacent HeLa cells expressing RUSH-eGFP-WNT3A at indicated time points after biotin addition. From S7 video. (a) WNT3A is in the Golgi after biotin treatment. Inset showing WNT3A movement along filopodia from one WNT3A-high cell to another WNT3A-low cell (based on GFP intensities). Arrow shows filopodic bridge transporting WNT3A. (b) At t = 33:40, multiple vesicles are seen on the filopodia. (c) At t = 40:00. (d) Fluorescence photomicrographs of HeLa cells (expressing RUSH-mCherry-WNT3A and stained with CellMask Deep Blue membrane dye co-plated with RNF43-mutant HPAF-II cells stained with CellMask Deep Green membrane dye. From S9 Video (i) Inset showing filopodia extending from HeLa cell to HPAF-II cell at 14:16. (ii-iii) Inset showing WNT3A (mCherry) on the filopodia from HeLa cells moving to HPAF-II cells at indicated time points.

https://doi.org/10.1371/journal.pone.0212711.g005

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current11:31, 12 April 2019Thumbnail for version as of 11:31, 12 April 20191,280 × 1,300 (1.73 MB)Z8600021 (talk | contribs)==WNT3A transfer via filopodia== (a-c) Fluorescence photomicrographs of adjacent HeLa cells expressing RUSH-eGFP-WNT3A at indicated time points after biotin addition. From S7 video. (a) WNT3A is in the Golgi after biotin treatment. Inset showing WNT3A...

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