File:Neutrophil extracellular trap 01.mp4
Neutrophil_extracellular_trap_01.mp4 (file size: 1.57 MB, MIME type: video/mp4)
Reference
<pubmed>22922410</pubmed> Nat Med. 2012 Sep;18(9):1386-93.
Infection-induced NETosis is a dynamic process involving neutrophil multitasking in vivo.
Yipp BG1, Petri B, Salina D, Jenne CN, Scott BN, Zbytnuik LD, Pittman K, Asaduzzaman M, Wu K, Meijndert HC, Malawista SE, de Boisfleury Chevance A, Zhang K, Conly J, Kubes P.
Abstract
Neutrophil extracellular traps (NETs) are released as neutrophils die in vitro in a process requiring hours, leaving a temporal gap that invasive microbes may exploit. Neutrophils capable of migration and phagocytosis while undergoing NETosis have not been documented. During Gram-positive skin infections, we directly visualized live polymorphonuclear cells (PMNs) in vivo rapidly releasing NETs, which prevented systemic bacterial dissemination. NETosis occurred during crawling, thereby casting large areas of NETs. NET-releasing PMNs developed diffuse decondensed nuclei, ultimately becoming devoid of DNA. Cells with abnormal nuclei showed unusual crawling behavior highlighted by erratic pseudopods and hyperpolarization consistent with the nucleus being a fulcrum for crawling. A requirement for both Toll-like receptor 2 and complement-mediated opsonization tightly regulated NET release. Additionally, live human PMNs injected into mouse skin developed decondensed nuclei and formed NETS in vivo, and intact anuclear neutrophils were abundant in Gram-positive human abscesses. Therefore early in infection NETosis involves neutrophils that do not undergo lysis and retain the ability to multitask.
PMID 22922410
Copyright
Reprinted by permission from Macmillan Publishers Ltd: Nat Med. (2012 Sep;18(9):1386-93.), copyright (2012)
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3560000586020
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Feb 01, 2015
Licensed content publisher
Nature Publishing Group
Licensed content publication
Nature Medicine
Licensed content title
Infection-induced NETosis is a dynamic process involving neutrophil multitasking in vivo
Licensed content author
Bryan G Yipp, Björn Petri, Davide Salina, Craig N Jenne, Brittney N V Scott, Lori D Zbytnuik
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Aug 26, 2012
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18
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9
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Fig 5, Fig 6, Supp video
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https://embryology.med.unsw.edu.au
Name of internet owner
Mark Hill
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current | 15:21, 1 February 2015 | (1.57 MB) | Z8600021 (talk | contribs) | Nat Med. 2012 Sep;18(9):1386-93. Infection-induced NETosis is a dynamic process involving neutrophil multitasking in vivo. Yipp BG1, Petri B, Salina D, Jenne CN, Scott BN, Zbytnuik LD, Pittman K, Asaduzzaman M, Wu K, Meijndert HC, Malawista SE, de Bois... |
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