Talk:Somatic Cell Nuclear Transfer
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Cite this page: Hill, M.A. (2026, October 20) Embryology Somatic Cell Nuclear Transfer. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Somatic_Cell_Nuclear_Transfer |
2012
Lessons learned from cloning dogs
Reprod Domest Anim. 2012 Aug;47 Suppl 4:115-9. doi: 10.1111/j.1439-0531.2012.02064.x.
Kim M, Oh H, Kim G, Park J, Park E, Jang G, Ra J, Kang S, Lee B. Source Department of Theriogenology and Biotechnology, Seoul National University, Seoul, Korea Stem Cell Research Center, RNL BIO, Seoul, Korea.
Abstract
The aim of this article is to review dog cloning research and to suggest its applications based on a discussion about the normality of cloned dogs. Somatic cell nuclear transfer was successfully used for production of viable cloned puppies despite limited understanding of in vitro dog embryo production. Cloned dogs have similar growth characteristics to those born from natural fertilization, with no evidence of serious adverse effects. The offspring of cloned dogs also have similar growth performance and health to those of naturally bred puppies. Therefore, cloning in domestic dogs can be applied as an assisted reproductive technique to conserve endangered species, to treat sterile canids or aged dogs, to improve reproductive performance of valuable individuals and to generate disease model animals. © 2012 Blackwell Verlag GmbH.
PMID 22827359
Segregation of donor cell mitochondrial DNA in gaur-bovine interspecies somatic cell nuclear transfer embryos, fetuses and an offspring
Mitochondrion. 2012 Jul 21;12(5):506-513. [Epub ahead of print]
Imsoonthornruksa S, Srirattana K, Phewsoi W, Tunwattana W, Parnpai R, Ketudat-Cairns M. Source Embryo Technology and Stem Cell Research Center, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Abstract
The fate of foreign mitochondrial DNA (mtDNA) following somatic cell nuclear transfer (SCNT) is still controversial. In this study, we examined the transmission of the heteroplasmic mtDNA of gaur donor cells and recipient bovine oocytes to an offspring and aborted and mummified fetuses at various levels during the development of gaur-bovine interspecies SCNT (iSCNT) embryos. High levels of the donor cell mtDNA were found in various tissue samples but they did not have any beneficial effect to the survival of iSCNT offspring. However, the factors on mtDNA inheritance are unique for each iSCNT experiment and depend on the recipient oocyte and donor cell used, which might play an important role in the efficiency of iSCNT. Copyright © 2012 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
PMID 22824460
