Template talk:Cahalan: Difference between revisions
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* Green - B lymphocytes labelled with antibody to B220 (fixed tissue) | * Green - B lymphocytes labelled with antibody to B220 (fixed tissue) | ||
Original template | |||
:'''Links:''' [[Immune System Development]] | [[Lymph Node Development]] | [[Quicktime_Movies|Quicktime]] | [[Flash_Movies|Flash]] | |||
:'''Movie Source:''' Michael D. Cahalan, Professor and Chair, Department of Physiology and Biophysics, University of California, Irvine. [http://crt.biomol.uci.edu Lab] Two-photon imaging movies reproduced with permission of Prof. Cahalan. Movies show lymphocyte traffic through adult lymph nodes. Cells were isolated from spleens and lymph nodes of BALB/c mice. | |||
:'''T Cell MP4:''' [[Quicktime_Movie_-_Mouse_Lymph_Node_1|Transendothelial migration]] | [[Quicktime_Movie_-_Mouse_Lymph_Node_2|T cell zone]] | [[Quicktime_Movie_-_Mouse_Lymph_Node_3|Medullary sinus]] | [[Quicktime_Movie_-_Mouse_Lymph_Node_4|Sinus endothelial barrier]] | [[Quicktime_Movie_-_Mouse_Lymph_Node_5|Bi-directional traffic]] | [[Quicktime_Movie_-_Mouse_Lymph_Node_6|cross the sinus endothelial barrier]] | |||
:'''T Cell Quicktime:''' [[Quicktime_Movie_-_Mouse_Lymph_Node_1|Transendothelial migration]] | [[Quicktime_Movie_-_Mouse_Lymph_Node_2|T cell zone]] | [[Quicktime_Movie_-_Mouse_Lymph_Node_3|Medullary sinus]] | [[Quicktime_Movie_-_Mouse_Lymph_Node_4|Sinus endothelial barrier]] | [[Quicktime_Movie_-_Mouse_Lymph_Node_5|Bi-directional traffic]] | [[Quicktime_Movie_-_Mouse_Lymph_Node_6|cross the sinus endothelial barrier]] | |||
:'''T Cell Flash:''' [[Movie_-_Mouse_Lymph_Node_1|Transendothelial migration]] | [[Movie_-_Mouse_Lymph_Node_2|T cell zone]] | [[Movie_-_Mouse_Lymph_Node_3|Medullary sinus]] | [[Movie_-_Mouse_Lymph_Node_4|Sinus endothelial barrier]] | [[Movie_-_Mouse_Lymph_Node_5|Bi-directional traffic]] | [[Movie_-_Mouse_Lymph_Node_6|Cross the sinus endothelial barrier]] | |||
'''References:''' | |||
<pubmed>16273098</pubmed>| [http://www.nature.com/ni/journal/v6/n12/full/ni1269.htmlNature Immunology] | |||
<pubmed>18173372</pubmed> | |||
Revision as of 17:33, 3 March 2013
Lymphocyte Migration Speeds
T cells - 10–12 μm/min in the follicle diffuse cortex, peak velocities up to 30 μm/min.
B cells - 6 μm/min in the follicle diffuse cortex, peak velocities up to 20 μm/min.
Both cortical T cells and follicular B cells move in random directions following "guide cells".
Lymphocyte Guide Cells
FDC - Follicular Dendritic Cells, may guide B cells in the follicle.
FRC - Fibroblastic Reticular Cells, may guide T cells in the follicle.
Lymph Node 2-Photon labelling
- Red - Alexa Fluor 633–conjugated WGA image channel
- sinus space both as a weakly stained area in the WGA image channel
- Green - T lymphocytes labeled with 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester.
- Green - B lymphocytes labelled with antibody to B220 (fixed tissue)
Original template
- Links: Immune System Development | Lymph Node Development | Quicktime | Flash
- Movie Source: Michael D. Cahalan, Professor and Chair, Department of Physiology and Biophysics, University of California, Irvine. Lab Two-photon imaging movies reproduced with permission of Prof. Cahalan. Movies show lymphocyte traffic through adult lymph nodes. Cells were isolated from spleens and lymph nodes of BALB/c mice.
- T Cell MP4: Transendothelial migration | T cell zone | Medullary sinus | Sinus endothelial barrier | Bi-directional traffic | cross the sinus endothelial barrier
- T Cell Quicktime: Transendothelial migration | T cell zone | Medullary sinus | Sinus endothelial barrier | Bi-directional traffic | cross the sinus endothelial barrier
- T Cell Flash: Transendothelial migration | T cell zone | Medullary sinus | Sinus endothelial barrier | Bi-directional traffic | Cross the sinus endothelial barrier
References: <pubmed>16273098</pubmed>| Immunology <pubmed>18173372</pubmed>
