Talk:Somatic Cell Nuclear Transfer

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On this website the Discussion Tab or "talk pages" for a topic has been used for several purposes: References - recent and historic that relates to the topic Additional topic information - currently prepared in draft format Links - to related webpages Topic page - an edit history as used on other Wiki sites Lecture/Practical - student feedback Student Projects - online project discussions. Links: Pubmed Most Recent | Reference Tutorial | Journal Searches Glossary Links Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link Cite this page: Hill, M.A. (2026, October 20) Embryology Somatic Cell Nuclear Transfer. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Somatic_Cell_Nuclear_Transfer

2012

Lessons learned from cloning dogs

Reprod Domest Anim. 2012 Aug;47 Suppl 4:115-9. doi: 10.1111/j.1439-0531.2012.02064.x.

Kim M, Oh H, Kim G, Park J, Park E, Jang G, Ra J, Kang S, Lee B. Source Department of Theriogenology and Biotechnology, Seoul National University, Seoul, Korea Stem Cell Research Center, RNL BIO, Seoul, Korea.

Abstract

The aim of this article is to review dog cloning research and to suggest its applications based on a discussion about the normality of cloned dogs. Somatic cell nuclear transfer was successfully used for production of viable cloned puppies despite limited understanding of in vitro dog embryo production. Cloned dogs have similar growth characteristics to those born from natural fertilization, with no evidence of serious adverse effects. The offspring of cloned dogs also have similar growth performance and health to those of naturally bred puppies. Therefore, cloning in domestic dogs can be applied as an assisted reproductive technique to conserve endangered species, to treat sterile canids or aged dogs, to improve reproductive performance of valuable individuals and to generate disease model animals. © 2012 Blackwell Verlag GmbH.

PMID 22827359

Segregation of donor cell mitochondrial DNA in gaur-bovine interspecies somatic cell nuclear transfer embryos, fetuses and an offspring

Mitochondrion. 2012 Jul 21;12(5):506-513. [Epub ahead of print]

Imsoonthornruksa S, Srirattana K, Phewsoi W, Tunwattana W, Parnpai R, Ketudat-Cairns M. Source Embryo Technology and Stem Cell Research Center, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.

Abstract

The fate of foreign mitochondrial DNA (mtDNA) following somatic cell nuclear transfer (SCNT) is still controversial. In this study, we examined the transmission of the heteroplasmic mtDNA of gaur donor cells and recipient bovine oocytes to an offspring and aborted and mummified fetuses at various levels during the development of gaur-bovine interspecies SCNT (iSCNT) embryos. High levels of the donor cell mtDNA were found in various tissue samples but they did not have any beneficial effect to the survival of iSCNT offspring. However, the factors on mtDNA inheritance are unique for each iSCNT experiment and depend on the recipient oocyte and donor cell used, which might play an important role in the efficiency of iSCNT. Copyright © 2012 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

PMID 22824460