File:Human CS13-15 otic vesicle 01.jpg: Difference between revisions

Revision as of 12:13, 6 April 2018

Laser-microdissection of FFPE-human inner ear slides. (A) FFPE slides containing embryonic tissues. (B) Coronal section of a fetus with highlighted regions of interest (dashed black circles, CVG, NC, and OV). Further images are provided showing tissue before (C, E) and after (D, F) laser-captured microdissection of CVG (dashed white lines in D) and NC tissues (dashed white line in F). Other developmental regions also are highlighted. CVG: cochlear-vestibular ganglions, OV: otic vesicle, NC: neural crest; GG: geniculate ganglion, G-CVG: geniculate- cochleovestibular ganglions, E: epithelium; and NT: neural tube. Scale bar = 100 μm (B) and 150 μm (C-F). https://doi.org/10.1371/journal.pone.0191452.g001

Reference

Chadly DM, Best J, Ran C, Bruska M, Woźniak W, Kempisty B, Schwartz M, LaFleur B, Kerns BJ, Kessler JA & Matsuoka AJ. (2018). Developmental profiling of microRNAs in the human embryonic inner ear. PLoS ONE , 13, e0191452. PMID: 29373586 DOI. Fig 1.

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	12:12, 6 April 2018		1,574 × 2,053 (457 KB)	Z8600021 (talk \| contribs)	* '''Developmental profiling of microRNAs in the human embryonic inner ear'''{{#pmid:29373586\|PMID29373586}} "Due to the extreme inaccessibility of fetal human inner ear tissue, defining of the microRNAs (miRNAs) that regulate development of the inner...

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Molecular Development - microRNA

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-* '''Developmental profiling of microRNAs in the human embryonic inner ear'''{{#pmid:29373586|PMID29373586}} "Due to the extreme inaccessibility of fetal human inner ear tissue, defining of the microRNAs (miRNAs) that regulate development of the inner ear has relied on animal tissue. In the present study, we performed the first miRNA sequencing of otic precursors in human specimens. Using HTG miRNA Whole Transcriptome assays, we examined miRNA expression in the cochleovestibular ganglion (CVG), neural crest (NC), and otic vesicle (OV) from paraffin embedded (FFPE) human specimens in the Carnegie developmental stages {{CS13}}, {{CS14}} and {{CS15}}. We found that in human embryonic tissues, there are different patterns of miRNA expression in the CVG, NC and OV. In particular, members of the miR-183 family (miR-96, miR-182, and miR-183) are differentially expressed in the CVG compared to NC and OV at Carnegie developmental stage {{CS13}}. We further identified transcription factors that are differentially targeted in the CVG compared to the other tissues from stages 13-15, and we performed gene set enrichment analyses to determine differentially regulated pathways that are relevant to CVG development in humans. These findings not only provide insight into the mechanisms governing the development of the human inner ear, but also identify potential signaling pathways for promoting regeneration of the spiral ganglion and other components of the inner ear." (More? [[Hearing - Inner Ear Development]])
+Laser-microdissection of FFPE-human inner ear slides. (A) FFPE slides containing embryonic tissues. (B) Coronal section of a fetus with highlighted regions of interest (dashed black circles, CVG, NC, and OV). Further images are provided showing tissue before (C, E) and after (D, F) laser-captured microdissection of CVG (dashed white lines in D) and NC tissues (dashed white line in F). Other developmental regions also are highlighted. CVG: cochlear-vestibular ganglions, OV: otic vesicle, NC: neural crest; GG: geniculate ganglion, G-CVG: geniculate- cochleovestibular ganglions, E: epithelium; and NT: neural tube. Scale bar = 100 μm (B) and 150 μm (C-F).
+https://doi.org/10.1371/journal.pone.0191452.g001
+===Reference===
+{{#pmid:29373586}}
+Fig 1.