Talk:Episcopic Fluorescence Image Capture

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On this website the Discussion Tab or "talk pages" for a topic has been used for several purposes: References - recent and historic that relates to the topic Additional topic information - currently prepared in draft format Links - to related webpages Topic page - an edit history as used on other Wiki sites Lecture/Practical - student feedback Student Projects - online project discussions. Links: Pubmed Most Recent | Reference Tutorial | Journal Searches Glossary Links Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link Cite this page: Hill, M.A. (2024, June 26) Embryology Episcopic Fluorescence Image Capture. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Talk:Episcopic_Fluorescence_Image_Capture

2002

Phenotyping transgenic embryos: a rapid 3-D screening method based on episcopic fluorescence image capturing

Nat Genet. 2002 Jan;30(1):59-65. Epub 2001 Dec 17.

Weninger WJ1, Mohun T.

Abstract

We describe a technique suitable for routine three-dimensional (3-D) analysis of mouse embryos that is based on episcopic fluorescence images captured during serial sectioning of wax-embedded specimens. We have used this procedure to describe the cardiac phenotype and associated blood vessels of trisomic 16 (Ts16) and Cited2-null mutant mice, as well as the expression pattern of an Myf5 enhancer/beta-galactosidase transgene. The consistency of the images and their precise alignment are ideally suited for 3-D analysis using video animations, virtual resectioning or commercial 3-D reconstruction software packages. Episcopic fluorescence image capturing (EFIC) provides a simple and powerful tool for analyzing embryo and organ morphology in normal and transgenic embryos.

PMID 11743576 DOI: 10.1038/ng785