File:Sheep follicle gene expression 01.jpg: Difference between revisions
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* '''black''' - genes expressed both in oocytes and granulosa cells. | * '''black''' - genes expressed both in oocytes and granulosa cells. | ||
| Distribution of the expression of the 24 biomarkers during early follicular development. These biomarkers correspond to the most strongly expressed genes in a specific cell type and stage (PDO, PMO, SCO, SAO, PDG, PMG, SCG, SAG). They were selected using pairwise comparisons (nbinom Test) comparing each stage to the others including multi-tissue samples (FDR<5% and a fold change >3 for granulosa samples and a fold change >-10 for oocyte samples). Their differential expression was confirmed by qRT-PCR. | Follicle type and diameter | ||
* primordial (PD) < 35 μM | |||
* primary (PM) 35 – 50 μM | |||
* secondary follicles (SC) 60 – 120 μM | |||
* small antral follicles (SA) 250 – 500 μM | |||
| Distribution of the expression of the 24 biomarkers during early follicular development. These biomarkers correspond to the most strongly expressed genes in a specific cell type and stage (PDO, PMO, SCO, SAO, PDG, PMG, SCG, SAG). | |||
They were selected using pairwise comparisons (nbinom Test) comparing each stage to the others including multi-tissue samples (FDR<5% and a fold change >3 for granulosa samples and a fold change >-10 for oocyte samples). Their differential expression was confirmed by qRT-PCR. | |||
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Latest revision as of 22:37, 12 November 2015
Sheep Follicle Gene Expression
Follicle type and diameter
|
Distribution of the expression of the 24 biomarkers during early follicular development. These biomarkers correspond to the most strongly expressed genes in a specific cell type and stage (PDO, PMO, SCO, SAO, PDG, PMG, SCG, SAG).
They were selected using pairwise comparisons (nbinom Test) comparing each stage to the others including multi-tissue samples (FDR<5% and a fold change >3 for granulosa samples and a fold change >-10 for oocyte samples). Their differential expression was confirmed by qRT-PCR. |
- Links: Granulosa cell | Oocyte Development | Sheep Development
Reference
<pubmed>26540452</pubmed>| PLoS One.
Copyright
© 2015 Bonnet et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Bonnet A, Servin B, Mulsant P, Mandon-Pepin B (2015) Spatio-Temporal Gene Expression Profiling during In Vivo Early Ovarian Folliculogenesis: Integrated Transcriptomic Study and Molecular Signature of Early Follicular Growth. PLoS ONE 10(11): e0141482. doi:10.1371/journal.pone.0141482
Fig 9. journal.pone.0141482.g009.jpg
Cite this page: Hill, M.A. (2024, June 25) Embryology Sheep follicle gene expression 01.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Sheep_follicle_gene_expression_01.jpg
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
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current | 22:11, 12 November 2015 | ![]() | 1,235 × 1,000 (168 KB) | Z8600021 (talk | contribs) | Fig 9. Distribution of the expression of the 24 biomarkers during early follicular development. These biomarkers correspond to the most strongly expressed genes in a specific cell type and stage (PDO, PMO, SCO, SAO, PDG, PMG, SCG, SAG). They were selec... |
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