File:Mouse lung development 01a.jpg: Difference between revisions

Latest revision as of 13:01, 28 September 2015

Histological analyses of Mouse Lungs at Various Embryonic Stages

Lung sections stained with H and E, and taken at various gestational stages as indicated.

The first two columns are at lower magnification (10X) and the last two columns at higher magnification (40X).
The first and third columns are representative sections from wild-type (+/+) embryos and the second and fourth columns from knockout (-/-) embryos.
No differences were visible at E14.5 or E16.5 (panel A, a-h). Significant differences were visible from E17.5 onwards (panel A, i-x).
Distal tubules showed dilation and mesenchyme thinning, with progression of septation in wild-type lungs (panel A, i, m and q), whereas the knockout lungs showed less saccular structures and more mesenchyme (panel A, j, n and r).
At higher magnifications of wild-type lungs (panel A, k, o and s), developing pre-alveoli and thinning mesenchyme (arrowheads) were seen but sections from knockout embryos showed a delay in this sequence of development (panel A, l, p and t).
At birth, lungs from knockout pups showed deficient septation and thick-walled mesenchyme (panel A, x, arrowheads).

Labels:

de - distal epithelium
m - mesenchyme
PA - pulmonary artery
a - pre-alveoli
b - bronchi

Original file name: 1471-213X-4-1-3.jpg http://www.biomedcentral.com/1471-213X/4/1/figure/F3 (Panel A cropped from original full image, resized to fit screen 800px)

Mouse lung development 01.jpg

Reference

<pubmed>15005800</pubmed>| BMC Developmental Biology

Yu et al. BMC Developmental Biology 2004 4:1 doi:10.1186/1471-213X-4-1

Copyright

© 2004 Yu et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

Cite this page: Hill, M.A. (2024, June 21) Embryology Mouse lung development 01a.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Mouse_lung_development_01a.jpg

What Links Here?

File history

Yi efo/eka'e gwa ebo wo le nyangagi wuncin ye kamina wunga tinya nan

	Gwalagizhi	Nyangagi	Dimensions	User	Comment
current	14:52, 25 August 2011		800 × 1,003 (495 KB)	S8600021 (talk \| contribs)	==Histological analyses of Mouse Lungs at Various Embryonic Stages== indicates lung sections stained with H and E, and taken at various gestational stages as indicated. The first two columns are at lower magnification (10X) and the last two columns at hi

You cannot overwrite this file.

File usage

There are no pages that use this file.

@@ Line 1: / Line 1: @@
 ==Histological analyses of Mouse Lungs at Various Embryonic Stages==
-indicates lung sections stained with H and E, and taken at various gestational stages as indicated. The first two columns are at lower magnification (10X) and the last two columns at higher magnification (40X). The first and third columns are representative sections from wild-type (+/+) embryos and the second and fourth columns from knockout (-/-) embryos. No differences were visible at E14.5 or E16.5 (panel A, a-h). Significant differences were visible from E17.5 onwards (panel A, i-x). Distal tubules showed dilation and mesenchyme thinning, with progression of septation in wild-type lungs (panel A, i, m and q), whereas the knockout lungs showed less saccular structures and more mesenchyme (panel A, j, n and r). At higher magnifications of wild-type lungs (panel A, k, o and s), developing pre-alveoli and thinning mesenchyme (arrowheads) were seen but sections from knockout embryos showed a delay in this sequence of development (panel A, l, p and t). At birth, lungs from knockout pups showed deficient septation and thick-walled mesenchyme (panel A, x, arrowheads). Labels: de, distal epithelium; m, mesenchyme; PA, pulmonary artery; a, pre-alveoli; b, bronchi. Panel B shows the morphometric analyses of lung terminal sac spaces in lungs at various gestational stages. A significant reduction in the terminal sac space was noted from E17.5 onwards. Results are expressed as mean ± s.d.
+Lung sections stained with H and E, and taken at various gestational stages as indicated.
+* The first two columns are at lower magnification (10X) and the last two columns at higher magnification (40X).
+* The first and third columns are representative sections from wild-type (+/+) embryos and the second and fourth columns from knockout (-/-) embryos.
+* No differences were visible at E14.5 or E16.5 (panel A, a-h). Significant differences were visible from E17.5 onwards (panel A, i-x).
+* Distal tubules showed dilation and mesenchyme thinning, with progression of septation in wild-type lungs (panel A, i, m and q), whereas the knockout lungs showed less saccular structures and more mesenchyme (panel A, j, n and r).
+* At higher magnifications of wild-type lungs (panel A, k, o and s), developing pre-alveoli and thinning mesenchyme (arrowheads) were seen but sections from knockout embryos showed a delay in this sequence of development (panel A, l, p and t).
+* At birth, lungs from knockout pups showed deficient septation and thick-walled mesenchyme (panel A, x, arrowheads).
+'''Labels:'''
+* '''de''' - distal epithelium
+* '''m''' - mesenchyme
+* '''PA''' - pulmonary artery
+* '''a''' - pre-alveoli
+* '''b''' - bronchi
 Original file name: 1471-213X-4-1-3.jpg http://www.biomedcentral.com/1471-213X/4/1/figure/F3 (Panel A  cropped from original full image, resized to fit screen 800px)
@@ Line 13: / Line 27: @@
 Yu et al. BMC Developmental Biology 2004 4:1   doi:10.1186/1471-213X-4-1
+====Copyright====
+© 2004 Yu et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
-© 2004 Yu et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
+{{Footer}}
 [[Category:Mouse]] [[Category:Respiratory]] [[Category:Histology]] [[Category:Abnormal Development]]
 [[Category:Mouse E14.5]] [[Category:Mouse E16.5]] [[Category:Mouse E17.5]] [[Category:Mouse E18.5]] [[Category:Mouse E20.5]]
+[[Category:Knockout Mouse]]