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==Maintenance of Human embryonic stem (ES) cells under the completely chemically defined condition==
==Human Embryonic Stem (ES) Cells Differentiation==


Characterization of teratomas from H9 cells cultured on an hE-cad-Fc-coated surface.  
Maintenance of Human embryonic stem (ES) cells under the completely chemically defined conditions. Characterization of teratomas from H9 cells cultured on an hE-cad-Fc-coated surface.  


Hematoxylin and eosin staining of paraffin sections through teratomas identified the differentiation huES cells into various tissues:
Hematoxylin and eosin staining of paraffin sections through teratomas identified the differentiation huES cells into various tissues:
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* '''g''' - ([[:File:Human embryonic stem cell defined conditions 03.jpg|larger image]]) contains neural tissue (1), cartilage (2), bone parenchyma (3), and epithelial tissue (4).  
* '''g''' - ([[:File:Human embryonic stem cell defined conditions 03.jpg|larger image]]) contains neural tissue (1), cartilage (2), bone parenchyma (3), and epithelial tissue (4).  


Bar indicates 100 μm.
 
Bar indicates 100 μm. {{HE}}




Original file name: Figure 2.  1471-213X-10-60-2-l.jpg http://www.biomedcentral.com/1471-213X/10/60/figure/F2 (Image cropped and resized from panel H of [[:File:Human embryonic stem cell defined conditions 01.jpg|full figure]])
Original file name: Figure 2.  1471-213X-10-60-2-l.jpg http://www.biomedcentral.com/1471-213X/10/60/figure/F2 (Image cropped and resized from panel H of [[:File:Human embryonic stem cell defined conditions 01.jpg|full figure]])


==Reference==
===Reference===
<pubmed>20525219</pubmed>| [http://www.biomedcentral.com/1471-213X/10/60 BMC Dev Biol.]
<pubmed>20525219</pubmed>| [http://www.biomedcentral.com/1471-213X/10/60 BMC Dev Biol.]



Latest revision as of 13:58, 23 February 2013

Human Embryonic Stem (ES) Cells Differentiation

Maintenance of Human embryonic stem (ES) cells under the completely chemically defined conditions. Characterization of teratomas from H9 cells cultured on an hE-cad-Fc-coated surface.

Hematoxylin and eosin staining of paraffin sections through teratomas identified the differentiation huES cells into various tissues:

  • a - immature neuroblastic tissue with neuronal rosettes
  • b - neuroepithelium with pigment
  • c - immature sebaceous tissue
  • d - cartilage
  • e - columnar epithelium
  • f - gut-like epithelial structures
  • g - (larger image) contains neural tissue (1), cartilage (2), bone parenchyma (3), and epithelial tissue (4).


Bar indicates 100 μm. (Stain - Haematoxylin Eosin)


Original file name: Figure 2. 1471-213X-10-60-2-l.jpg http://www.biomedcentral.com/1471-213X/10/60/figure/F2 (Image cropped and resized from panel H of full figure)

Reference

<pubmed>20525219</pubmed>| BMC Dev Biol.


© 2010 Nagaoka et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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