Talk:Developmental Signals - Slit2/Robo1

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Cite this page: Hill, M.A. (2020, January 20) Embryology Developmental Signals - Slit2/Robo1. Retrieved from


Alterted SLIT2/ROBO1 signalling is linked to impaired placentation of missed and threatened miscarriage in early pregnancy

Histopathology. 2017 Oct;71(4):543-552. doi: 10.1111/his.13250. Epub 2017 Jul 11.

Li P1,2, Shi Y1, Shuai H3, Cai Y3, Lu W1, Wang G1, Gao L3, Wang L4, Fan X5, Yang X1.

Abstract AIMS: Two-thirds of early pregnancy failures present with reduced trophoblast invasion, and SLIT2/ROBO1 signalling is considered to play an important role in trophoblast function during pregnancy. We investigated SLIT2/ROBO1 signalling associated with missed and threatened miscarriage during early gestation. METHODS AND RESULTS: Human placenta samples were collected from women with missed miscarriage (n = 25), threatened miscarriage (n = 22) and termination of pregnancy controls (n = 32). Corresponding decreases in beta human chorionic gonadotrophin (β-hCG) levels and shallow trophoblast invasion were observed in patients with missed and threatened miscarriage, immunohistological staining revealed abnormal Slit2 and Robo1, as well as E-cadherin and activating protein-2 alpha (AP-2α) expression in villi and extravillous trophoblasts, and the expression of these proteins were confirmed in villi and decidua of miscarriage material by Western blotting. Using HTR8/SVneo cells, blocking SLIT2/ROBO1 signalling promoted cell migration, proliferation and suppressed differentiation. Moreover, blocking SLIT2/ROBO1 signalling in HTR8/SVneo cells altered trophoblast differentiation-related and angiogenesis-related gene mRNA expression, which also occurred in the tissues of missed and threatened miscarriage. CONCLUSIONS: SLIT2/ROBO1 signalling may regulate trophoblast differentiation and invasion causing restricting β-hCG production, shallow trophoblast invasion and inhibiting placental angiogenesis in missed and threatened miscarriage during the first trimester. © 2017 John Wiley & Sons Ltd. KEYWORDS: differentiation; invasion; miscarriage; trophoblast PMID: 28485101 DOI: 10.1111/his.13250

Progesterone down-regulates SLIT/ROBO expression in mouse corpus luteum

Acta Histochem. 2017 Sep;119(7):740-746. doi: 10.1016/j.acthis.2017.09.006. Epub 2017 Sep 23.

Zhang X1, Mi M2, Hao W1, Fan Q1, Gao B3.

Abstract BACKGROUND: Progesterone produced by the corpus luteum (CL) is essential for preparation, implantation and maintenance of gestation. Furthermore, progesterone plays a protective role against luteolysis in rodents. It has been reported that Slit/Robo family members expressed in the CL and involved in prostaglandin F2α (PGF2α) induced luteolysis. However, the interactions between progesterone and Slits/Robos in CL are not clear. This study was designed to examine whether or not luteolysis is regulated by the interaction of progesterone and Slits/Robos in mouse CL. METHODS: In the current study, we used Real-time PCR to identify the effect of progesterone on Slit2/Robo1 expression in cultured luteal cells in vitro, and the exogenous progesterone injection on mouse luteolysis and Slit/Robo expression in vivo was studied via Real-time PCR and Western bolt. RESULTS: Our in vitro experiment revealed that 1μM progesterone significantly decreased Slit2/Robo1 mRNA levels at 6h, 12h and 24h. Our in vivo experiment showed that the mRNA and protein levels of Slit2 and Robo1 decreased significantly 7days after progesterone supplement. CONCLUSION: These findings indicate that progesterone maintains CL function and resists luteolysis possibly through down-regulating Slit/Robo signaling pathway in the CL. Copyright © 2017 Elsevier GmbH. All rights reserved. KEYWORDS: Animal experiment; Luteolysis; Mouse; Progesterone; Slit/Robo PMID: 28947260 DOI: 10.1016/j.acthis.2017.09.006