Neutrophil extracellular trap Movie

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Adult Mouse Neutrophil Extracellular Trap

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In vivo intracellular imaging demonstrates PMNs with normal, diffuse and absent nuclei during a Gram-positive skin infection. Normal nuclei are easily distinguished by their distinct multilobar morphology.

  • Neutrophil - yellow.
  • Bacteria (S. aureus) - green.
  • Extracellular DNA Nets - red.
  • Intracellular DNA - blue.

Anuclear PMNs are alive and continue to migrate. Examples are demonstrated of anuclear PMNs engorged with GFP-staphylococcus as well as anuclear PMNs devoid of bacteria.

A PMN with an abnormal diffuse nucleus is highlighted for its multiple and erratic pseudopod formation and hyperpolarization. NETs are visualized within the area of anuclear PMNs.

(text from Supplementary video legend)

Links: Neutrophil chemotaxis movie | Cardiovascular System - Blood Development | Movies


Reference

<pubmed>23700386</pubmed>| PLoS Biol. | PLoS Biol. Immunobiology Collection

Copyright

Reprinted by permission from Macmillan Publishers Ltd: Nat Med. (2012 Sep;18(9):1386-93.), copyright (2012)

Original video altered in size and labelling. 592 × 408

  • License Number - 3560000586020
  • License date - Feb 01, 2015
  • Type of Use - post on a website
  • Requestor type - academic/university or research institute
  • Portion - figures/tables/illustrations
  • Homepage URL for posting content - https://embryology.med.unsw.edu.au
  • Name of internet owner - Mark Hill



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Cite this page: Hill, M.A. (2019, May 27) Embryology Neutrophil extracellular trap Movie. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Neutrophil_extracellular_trap_Movie

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© Dr Mark Hill 2019, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G